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Compound microbial inoculant for composting fermentation, solid-state fermentation production method for compound microbial inoculant and application of compound microbial inoculant

A composite microbial agent and solid-state fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the discontinuous production process of solid-state agent, the lack of scientific combination of agent, and the maturity of compost fermentation Low-level problems, to achieve the effect of a large total number of viable bacteria in the product, improved nitrogen retention effect, and low rate of miscellaneous bacteria

Active Publication Date: 2012-09-12
湖南德施普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In recent years, great progress has been made in the research of composting bacterial agents at home and abroad, and products of compound microbial preparations with different combinations have also appeared in China, but the common problem is that due to the lack of understanding of the mechanism of the composting process, the combination of microbial agents Lack of science, so that the degree of compost fermentation is low, affecting the quality of the product
In addition, the current production of microbial inoculum mostly adopts liquid fermentation, the product is liquid, the shelf life is short, and transportation is inconvenient; or some production uses solid substances to absorb liquid and then dry, the production process of the solid inoculum is discontinuous, resulting in a high rate of bacteria The rate of viable bacteria is low, which affects the effect of its application process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Composite microbial bacterial agent 1# solid-state fermentation production method

[0031] This embodiment includes the following steps:

[0032] (1) Primary seed fermentation: Inoculate the 14 strains contained in the bacterial agent 1# into 50ml shake flasks to expand culture according to their respective characteristic medium, and inoculate them into the primary seed fermentation tank according to the aseptic inoculation technique. The fermentation temperature is 55°C, the stirring speed is 150r / min, the cultivation time is 1.5 days, sterile air is introduced, the volume ratio of gas to material is 1:2.1 for 6 hours before fermentation, and the volume ratio of gas to material is 1:1.1 after that, to obtain a grade seed liquid;

[0033] The first-level seed fermentation tank is equipped with a liquid medium, and the mass ratio of each component of the liquid medium is: brown sugar 5%, starch 2%, ammonium sulfate 1%, sodium chloride 5%, calcium di...

Embodiment 2

[0038] Embodiment 2 Composite microbial bacterial agent 2# solid-state fermentation production method

[0039] This embodiment includes the following steps:

[0040] (1) Primary seed fermentation: Inoculate the 8 strains contained in the bacterial agent 2# into 50ml shake flasks for expansion (in which expansion liquid medium: beef extract peptone medium is used for high-temperature bacteria, and peptone medium for high-temperature mold is used PDA medium, high-temperature actinomycetes adopt improved Gao’s No. 1 medium, and high-temperature lactic acid bacteria adopt MRS medium), inoculate into the first-level seed fermentation tank according to the aseptic inoculation technique, the fermentation temperature is 35°C, and the stirring speed is 150r / min , the cultivation time is 2 days, sterile air is introduced, 6 hours before fermentation, the gas-to-material volume ratio is 1:2, and then the gas-to-material volume ratio is 1:1.1 to obtain the first-grade seed liquid;

[...

Embodiment 3

[0046] Example 3 Composting with sludge as the main raw material

[0047] This embodiment includes the following steps:

[0048] Compost raw material composition: sludge 60%, rice stalk 15%, compost return material (the semi-finished product after composting is used as return material) 25%;

[0049] The mass ratios of composite microbial agents 1# and 2# to compost raw materials were 4‰ respectively.

[0050] Composting process: First mix the compound microbial agent 1# with 20% rice straw evenly, then mix with the rest of the rice straw, sludge, and returned materials, and then pile it into a pile with a width of 6m, a height of 1.5m, and a length of different Limited semi-open stacks, the temperature of the materials in winter is raised to 50°C within 16 hours, the fermentation temperature is kept below 75°C, and the piles are turned every 2 to 3 days;

[0051] The material is cooled to 45°C within 11 days, and the temperature is maintained, and the bacterial agent 2# is i...

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Abstract

The invention discloses a compound microbial inoculant for composting fermentation, a solid-state fermentation production method for the compound microbial inoculant and application of the compound microbial inoculant. The compound microbial inoculant for the composting fermentation comprises a No.1 inoculant and an optional No.2 inoculant, wherein the No.1 inoculant and the No.2 inoculant are applicable to primary fermentation and secondary fermentation respectively; and the No.1 inoculant is a high-temperature microbial flora which can resist the temperature of 50 to 75 DEG C, and the No.2 inoculant is a medium-temperature microbial flora which can resist the temperature of 10 to 45 DEG C. The invention also discloses the solid-state fermentation production method for the compound microbial inoculant for the composting fermentation and the application of the compound microbial inoculant. Various strains in the compound microbial inoculant for the composting fermentation can synergistically symbiose well; by the solid-state fermentation production method for the compound microbial inoculant, the product has a large quantity of viable bacteria, is low in percentage of contaminating microorganism and high in stability, and has a long quality guarantee period; and the composting which is performed by using the compound microbial inoculant is favorable for improving the maturity of materials.

Description

technical field [0001] The present invention relates to a composite microbial bacterial agent and its fermentation production method and application, in particular to a composite microbial bacterial agent used for composting waste organic materials such as sludge, livestock manure and agricultural straw, and its solid-state fermentation production method and application. Background technique [0002] The research on composting bacterial agents abroad began more than half a century ago, and has been widely used in Japan, the United States, South Korea, and Taiwan. [0003] The composting process is a process of growth and decline of microbial ecological communities. In this process, with the change of environmental conditions such as temperature and pH value, the number of microbial populations will also change, and different dominant bacteria will be formed in different fermentation stages. group, thereby degrading and digesting different organic matter. Throughout the com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F17/00C12R1/07C12R1/10C12R1/225C12R1/46C12R1/01C12R1/66C12R1/68C12R1/885C12R1/645
CPCY02W30/40
Inventor 孙俊荣陈祥国杨云凤
Owner 湖南德施普生物科技有限公司
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