Novel application of cicada fungus to preparation of medicament for treating dermatophytosis
A technology for cicadae and beriberi, which is applied in the direction of drug combination, microorganism, and pharmaceutical formula
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Embodiment 1
[0038] The cultivation of embodiment 1 artificial cicadae
[0039] 1) Strain preparation:
[0040] Slant seed preparation: transplant the Paecilomyces cicadae strain into a PSA medium slant test tube, cultivate it at 22°C to 25°C for 5 to 7 days, and select a well-growing seed and save it for later use;
[0041] Preparation of the first-level seed: insert the slanted seed into the potato sucrose culture solution, and cultivate it for 3-7 days at 22°C-25°C with an oscillation frequency of 130-150r / min until a large number of mycelial balls appear in the conical flask Later, it will be used as a first-class reserve;
[0042] The PSA medium contains the following components (contained in every 1000ml of medium): 200g of potatoes, 20g of sucrose, and 20g of agar. The preparation method is: put diced peeled potatoes in a container, add 1000ml of water, and boil for 20min to 30min , filter, add sucrose and agar, make up water to 1000ml, heat to dissolve, then pack into test tubes,...
Embodiment 2
[0060] The preparation of embodiment 2 cicadae active ingredient extract
[0061] Collect the mixture of the spore bundles, conidia, mycelium and the remaining substance of the culture medium obtained by the culture method of Example 1, and obtain the water extract of the mixture by decoction, and adjust it to 1.43g / ml by conventional methods (Based on crude drug amount) liquid medicine, adjust pH to 6.0.
Embodiment 3
[0062] Embodiment 3 cicada flower in vitro antifungal test
[0063] 1 material
[0064] Medicine: the cicadae active ingredient extract that embodiment 2 prepares, and concentration is 1.43g / ml (by crude drug amount), and pH is 6.0, and 10 pounds 20min are sterilized; Fluconazole (batch number 940112, produced by Chongqing Institute of Medicine) , prepared into a 20mg / ml solution (pH6.0); strains: 5 fungal strains were purchased from Nanjing Institute of Dermatology, Chinese Academy of Medical Sciences
[0065] 2 Methods and Results
[0066] Candida albicans was cultured at 20-25°C for 24 hours and diluted with sodium chloride injection; Epidermophyton flocciformis, S. lanoides, Trichophyton gypsum and Trichophyton rubrum were all treated with 20 The slant culture cultured at ~25°C for 7 days was washed with sodium chloride injection, shaken, filtered, microscopically counted, and diluted to make the final concentration of the bacterial solution 1×10 5 CFU / ml, the minimum i...
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