Chinese medicinal compound with tongue squamous cell carcinoma resisting effect and preparation method thereof
The technology of tongue squamous cell carcinoma and traditional Chinese medicine is applied in the application field of preparing a medicine for treating tongue phosphorus cancer, which can solve the problems of toxic and side effects, poor prognosis, influence on patients' life, health and quality of life, etc., and achieves the effect of convenient clinical use.
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Embodiment 1
[0020] 1. A Chinese medicine compound with anti-tongue squamous cell carcinoma effect, which is made from the following raw materials in parts by weight:
[0021] 10 parts of winter melon seeds, 12 parts of French Pinellia, 10 parts of Dannanxing, 12 parts of shanzake mushrooms, 10 parts of cat's claw, 10 parts of golden buckwheat, 10 parts of Houttuynia cordata, 15 parts of agrimony, 12 parts of sumac, red 15 parts of flowers, 10 parts of Radix Ginseng, 10 parts of Polygonatum Polygonatum, and 10 parts of Ophiopogon japonicus.
[0022] 2. The preparation method of the Chinese medicine compound with anti-tongue squamous cell carcinoma effect is:
[0023] Take 10 parts of winter melon seeds, 12 parts of Pinellia chinensis, 10 parts of Dannanxing, 12 parts of shanzi mushroom, 10 parts of cat's claw, 10 parts of golden buckwheat, 10 parts of Houttuynia cordata, 15 parts of agrimony, 12 parts of sumac, Add 15 parts of safflower, 10 parts of Radix Ginseng, 10 parts of Polygonatum ...
Embodiment 2
[0025] 1. A Chinese medicine compound with anti-tongue squamous cell carcinoma effect, which is made from the following raw materials in parts by weight:
[0026] 20 parts of winter melon seeds, 24 parts of French Pinellia, 20 parts of Dannanxing, 24 parts of shanzi mushroom, 20 parts of cat's claw, 20 parts of golden buckwheat, 20 parts of Houttuynia cordata, 30 parts of agrimony, 24 parts of sumac, red 30 parts of flowers, 20 parts of Radix Ginseng, 20 parts of Polygonatum Polygonatum, and 20 parts of Ophiopogon japonicus.
[0027] 2. The preparation method of the Chinese medicine compound with anti-tongue squamous cell carcinoma effect is:
[0028] Take 20 parts of winter melon seeds, 24 parts of Pinellia chinensis, 20 parts of Dannanxing, 24 parts of sage mushrooms, 20 parts of cat's claw, 20 parts of golden buckwheat, 20 parts of Houttuynia cordata, 30 parts of agrimony, 24 parts of sumac, Add 30 parts of safflower, 20 parts of Radix Ginseng, 20 parts of Polygonatum Poly...
Embodiment 3
[0029] Example 3 Experimental research on anti-lingual phosphorus cancer
[0030] 1. Materials
[0031] 1.1 Cell lines: SAS cells were donated by Professor Zheng Jinhua of Harbin Medical University. The tongue squamous cell line TCA-8113 was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. It was passaged and preserved in our laboratory.
[0032] 1.2 Drugs: the Chinese medicine compound extract provided in Embodiment 1 of the present invention.
[0033] 1.3 The main reagents are penicillin (Shandong Lukang), streptomycin sulfate (Amresco), MTT (thiazole salt, Genebase), trypsin (Nanjing Shengxing), EDTA (Sigma), RPMI-1640 medium (GIBCO), iodide Propidium (PI, Sigma, USA), RPMI-1640 (GIBCO), newborn calf serum (Lanzhou Minhai, Hyclone joint venture), 6-well culture plate and 24-well plate (Corning product). PEG2 detection ELISA kit (R & D company).
[0034] 1.4 Instruments Ultra-clean bench (Sujing Company, SW-CJ-1F), CO 2Incubator (Thermo, USA), in...
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