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Formula technology for agrocybe chaxingu strain culture medium and process for culturing strain

A kind of culture medium for bacteria and the technology of tea salamander, which is applied in horticulture, botanical equipment and methods, fertilizer mixture, etc., can solve the problems of easy pollution, improper cultivation process, and insufficient mycelium, and achieve the improvement of biotransformation rate, The effect of reducing pollution rate and outstanding adaptability

Inactive Publication Date: 2012-09-19
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the deficiencies in the production process of the strains: 1. The formula of the strain culture medium is not good, and the nutritional ingredients are not properly equipped, resulting in the lack of necessary nutritional supplements for the growth of the strain mycelium, the mycelium is not strong enough, and the ability to resist miscellaneous bacteria Poor, easy to pollute, the yield of strains can only reach about 70%
2. The production and cultivation process of the strains is not appropriate, and it also affects the growth of the strains during the growth of the mycelia, making the output of the mushrooms unsatisfactory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of culture medium formulation technology of tea Salmonella fungus strain and the production and cultivation process of strain, the specific steps are as follows:

[0024] a. Culture medium formula: choose 75% cottonseed hulls, 10% sawdust, 12% wheat husks, 3% lime, 60% water content, PH7-9 with a material-to-water ratio of 1:1-1.2.

[0025] b. Bagging: After mixing the above raw material ratios evenly, use a 12×24cm polypropylene plastic bag to cover tightly with a plastic collar, and each bag contains 400 grams;

[0026] c. Control of the time from mixing materials to sterilization: in the high temperature season, the temperature is above 23°C, and the time is controlled within 3 hours; the temperature is below 23°C, and the time is controlled within 4 hours.

[0027] d. Sterilization: First check whether the water in the pot is sufficient. If the water is insufficient, add enough water first, then put the prepared medium into the sterilizing pot, cover the pot,...

Embodiment 2

[0034] A kind of culture medium formulation technology of tea Salmonella fungus strain and the production and cultivation process of strain, the specific steps are as follows:

[0035] a. Culture medium formula: choose cottonseed hull 86%, wheat husk 15%, lime 3%, material water ratio 1:1-1.2, water content 60%, pH 7-9;

[0036] b. Bagging: After mixing the above raw material ratios evenly, use a 12×24cm polypropylene plastic bag to cover tightly with a plastic collar, and each bag contains 400 grams;

[0037] c. Control of the time from mixing materials to sterilization: in high temperature seasons, when the temperature is above 23°C, the time is controlled within 3 hours; when the temperature is below 23°C, the time is controlled within 4 hours;

[0038] d. Sterilization: First check whether the water in the pot is sufficient. If the water is insufficient, add enough water first, then put the prepared medium into the sterilizing pot, cover the pot, tighten the screws, and cl...

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Abstract

The invention discloses a formula technology for a grocybe chaxingu strain culture medium. The technology is characterized in that the grocybe chaxingu strain culture medium comprises the following components in percentage by weight: 75 to 90 percent of cottonseed hull, 9 to 12 percent of sawdust, 12 to 18 percent of wheat bran, and 2 to 5 percent of lime, 60 percent of water content, and PH value 7-9; the technology comprises the following steps of: mixing the components according to the formula of the culture medium; bagging; stirring and controlling sterilization time; sterilizing; disinfecting; inoculating; culturing the strain, wherein the temperature of a strain culture room is 23 to 26 DEG C, the relative humidity of air is less than 70 percent, the strain is cultured in darkness, and ventilating every morning and evening for 60 minutes respectively; checking the strain; and selling. The PH value is 7 to 9, so that the pollution rate of hypha is reduced obviously, the strain is obvious in stability and adaptability, and the yield of the strain can be improved from 70 to 98 percent; and according to the process for culturing the strain, the quality of the strain is greatly improved, the production and cultivation yield of grocybe chaxingu can be increased from 250 to 350g, and the biological conversion rate is improved by 40 percent.

Description

technical field [0001] The invention relates to a formula technology of a strain culture medium and a strain production and cultivation process, in particular to a formulation technology and a strain production and cultivation process of a novel tea salami mushroom strain culture medium. technical background [0002] Gutian County, Ningde City is the capital of edible fungi in China. In 1998, the artificial cultivation technology of A. saponica was introduced and the trial planting was successful. Due to the high nutritional value, good market price, good economic benefits and broad prospects, mushroom growers actively cultivated it. , becoming another main planting species in Gutian County and even the whole country after tremella and shiitake mushrooms. In 2006, the county's cultivation volume reached 300 million bags, and it increased at a rate of 12% every year, which led to the rapid development of tea mushroom cultivation. By 2011, the county's cultivation volume excee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00A01G1/04
Inventor 彭彪阮毅阮雁林雄平周逢芳雷莹刘春雷
Owner NINGDE NORMAL UNIV