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Single nucleotide polymorphism (SNP) mark relevant with milk production traits of Chinese Holstein dairy cattle and application thereof

A technology for dairy cows and traits, applied in the fields of genetic engineering and molecular biology, can solve the problems of space and development scale limitations, affecting the enthusiasm of dairy farmers, and achieve the effect of speeding up the breeding process and accurate detection methods.

Inactive Publication Date: 2012-09-19
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional development model of increasing the total milk production by expanding the number of dairy cows is easily limited by space and development scale, which affects the enthusiasm of dairy farmers

Method used

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  • Single nucleotide polymorphism (SNP) mark relevant with milk production traits of Chinese Holstein dairy cattle and application thereof
  • Single nucleotide polymorphism (SNP) mark relevant with milk production traits of Chinese Holstein dairy cattle and application thereof
  • Single nucleotide polymorphism (SNP) mark relevant with milk production traits of Chinese Holstein dairy cattle and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Acquisition of SNP markers related to milk production traits of Chinese Holstein dairy cows

[0022] 1.1 Extraction of blood DNA from Chinese Holstein cows

[0023] The bovine blood sample used in this example is coagulated bovine whole blood stored at -20°C. The whole blood is divided into blood clot and serum. The blood clot is dark red, and the serum is clear yellow. Mainly found in blood clots.

[0024] Use the Tiangen Blood DNA Extraction Kit to extract genomic DNA from blood clots, and make slight adjustments according to the kit instructions. The specific steps are as follows:

[0025] 1) Prepare 2mL sterilized round-bottom centrifuge tubes, and place a clean steel ball (about 5mm-6mm in diameter) in each tube;

[0026] 2) Take out the blood sample, and after thawing, use surgical scissors to cut about 200 μl-300 μl of the clot into a centrifuge tube. Add 600 μl of cell lysate CL, put it into a Qiangen tissue grinder, grind at a frequency of 30 times...

Embodiment 2

[0050] Example 2 Correlation analysis and detection application of different genotypes and milk production traits of Chinese Holstein cows

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Abstract

The invention provides a single nucleotide polymorphism (SNP) mark relevant with milk production traits of Chinese Holstein dairy cattle and application thereof. The mark is located in protein tyrosine kinase 2 (PTK2) gene of the Chinese Holstein dairy cattle, such as the position of 227 bp represented by SEQ ID NO.1, milk yield and protein content of the Chinese Holstein dairy cattle with bases T at the position are higher than those of the Chinese Holstein dairy cattle with bases G at the position, but the butter-fat content of the former is remarkably lower than that of the latter. The SNPmark relevant with the milk production traits of Chinese Holstein dairy cattle is screened by adopting a polymerase chain reaction (PCR) and a sequencing technology, the genotype of the Chinese Holstein dairy cattle to be detected is detected by means of a restriction fragment polymorphism (RFLP) method, breeding of advantageous variety of the Chinese Holstein dairy cattle with high milk yield isperformed according to the genotype, and accordingly the breeding course of the Chinese Holstein dairy cattle is accelerated.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a SNP marker related to milk production traits of Chinese Holstein cows and its application. Background technique [0002] The development of the dairy industry plays an important role in improving people's dietary structure, enhancing national physique, and promoting economic development. The dairy industry is the leading industry in the development of the dairy industry, and the development level of my country's dairy industry is obviously behind the developed countries in the world. The total milk production accounts for less than 6% of the world's total, and the per capita share is only a quarter of the world's average level. , the yield per unit area of ​​dairy cows is less than half of that of developed countries in the world. The traditional development model of increasing the total milk production by expanding the number of dairy cows is easily li...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 张勤刘剑锋张胜利王海飞赵红梅
Owner CHINA AGRI UNIV
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