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Enzyme-linked immunosorbent assay kit for human oxidized low-density lipoprotein, and using method and application

A low-density lipoprotein, enzyme-linked immunoassay technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., to achieve good market application prospects, high accuracy, and improve the effect of detection accuracy

Active Publication Date: 2014-06-11
成都华神生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, there is currently no simple, fast, non-invasive, low-cost and high-accuracy coronary heart disease diagnostic product in clinical practice, and it is necessary to develop new coronary heart disease detection methods.

Method used

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  • Enzyme-linked immunosorbent assay kit for human oxidized low-density lipoprotein, and using method and application
  • Enzyme-linked immunosorbent assay kit for human oxidized low-density lipoprotein, and using method and application

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Experimental program
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Effect test

Embodiment 1

[0058] Embodiment 1 The composition of detection kit of the present invention and its using method

[0059] 1. The composition of the kit

[0060] (1) A microtiter plate coated with anti-human oxidized low-density lipoprotein antibody;

[0061] (2) Human oxidized low-density lipoprotein series standard products:

[0062] Standard 1: Standard 1 contains 20g bovine serum albumin and 50g sucrose per liter;

[0063] Standard 2: Each liter of Standard 2 contains 20g of bovine serum albumin, 50g of sucrose and 1U of oxidized low-density lipoprotein;

[0064] Standard product 3: Each liter of standard product 3 contains 20g bovine serum albumin, 50g sucrose and 2U oxidized low-density lipoprotein;

[0065] Standard 4: Standard 4 contains 20g bovine serum albumin, 50g sucrose and 4U oxidized low-density lipoprotein per liter;

[0066] Standard 5: Standard 5 contains 20g bovine serum albumin, 50g sucrose and 8U oxidized low-density lipoprotein per liter;

[0067] Standard 6: Each ...

Embodiment 2

[0158] The clinical application of embodiment 2 kit of the present invention

[0159] Adopt kit of the present invention to detect 508 routine normal subjects and 176 routine coronary heart disease patients, the result shows, normal subjects plasma OxLDL content is 20.18 ± 15.02U / ml, coronary heart disease patient plasma OxLDL content is 68.90 ± 18.42U / ml ml, there is a significant difference between the two (<0.0001), which can quickly and accurately detect coronary heart disease.

[0160] In conclusion, the enzyme-linked immunosorbent assay kit provided by the present invention has high sensitivity and strong specificity, can be used for rapid and accurate clinical detection of coronary heart disease, is non-invasive, low in price, and has a good application prospect.

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Abstract

The invention provides an enzyme-linked immunosorbent assay (ELISA) kit for human oxidized low-density lipoprotein, which comprises the following ingredients: (1) an ELISA plate coated with an anti-human oxidized low-density lipoprotein antibody; (2) serial standard solution of human oxidized low-density lipoprotein; (3) an enzyme-labeled antibody; (4) diluting solution; (5) washing solution; (6) substrate solution; and (7) stopping solution. The invention further provides a method for using the kit and application of the kit. Compared with the traditional detection means for coronary heart disease, the kit has the characteristics of no wound, good simplicity, convenience and rapidness, high sensitivity and low cost.

Description

technical field [0001] The invention relates to a detection kit, in particular to a kit for detecting the content of OxLDL in a sample to be tested. Background technique [0002] Coronary heart disease, coronary atherosclerotic heart disease, its diagnosis is mainly based on the patient's typical medical history, clinical symptoms, combined with instrumentation and biochemical examination. At present, the commonly used examination methods include conventional electrocardiogram, electrocardiogram stress test, echocardiography, spiral CT and ultra-high-speed CT, coronary artery ultrasound imaging, intravascular ultrasound, nuclide myocardial imaging and selective coronary angiography, as well as laboratory tests Including myocardial enzyme examination, troponin, C-reactive protein, blood lipid and lipoprotein examination, etc. Most of the above inspection methods are based on the detection of changes in ECG signals (various ECGs) and changes in ventricular wall motion (echoca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/543
Inventor 徐燕华陈英万方
Owner 成都华神生物技术有限责任公司