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Fibroblast culture solution

A technology of fibroblasts and culture medium, applied in animal cells, vertebrate cells, artificial cell constructs, etc., can solve the problem that fibroblasts do not have a fibrous shape, the growth rate of fibroblasts is slow, and the ability to resist apoptosis is weak and other issues, to achieve the effect of not easy aging, strong proliferative ability, high quality and efficiency

Inactive Publication Date: 2012-09-26
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

The current conventional cell culture medium has the problems of slow growth rate and weak proliferation ability of fibroblasts in use, and the cultured fibroblasts often do not have typical fibrous morphology, weak anti-apoptosis ability and easy aging

Method used

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  • Fibroblast culture solution
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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] 1. Materials and methods

[0016] 1. Fibroblast culture

[0017] Fibroblasts from cattle, sheep, pigs and mice were cultured. The experimental group used a new designed fibroblast culture medium (hereinafter referred to as the new culture medium). The composition of the new culture medium was: 10% FBS + 4 ng / ml alkali Sexual fibroblast growth factor + 2.5 μg / ml Plasmocin + 100 U / ml penicillin + 100 μg / ml streptomycin + high glucose DMEM medium. The control group used conventional fibroblast culture medium (hereinafter referred to as conventional culture medium), the composition of which was: 10% FBS + 100 U / ml penicillin + 100 μg / ml streptomycin + high glucose DMEM medium. The fibroblasts of different species with the same culture passage were taken respectively, and cultured with the above two culture mediums.

[0018] 2. Detection of proliferation ability of fibroblasts

[0019] The fibroblasts of different species were inoculated at the same density, and the above...

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Abstract

The invention discloses a fibroblast culture solution. The fibroblast culture solution comprises the following components: 10% of fetal bovine serum, 4ng / ml of basic fibroblast growth factor, 2.5mu g / ml of Plasmocin, 100U / ml of penicillin and 100mu g / ml of streptomycin, and a high glucose DMEM (Dulbecco minimum essential medium) culture solution is made up to 100%. Compared with the conventional fibroblast culture solution, the fibroblast culture solution adopting the design disclosed by the invention can realize fast fibroblast growth speed and strong proliferation ability, and a cultured fibroblast has the typical fibrous form and stronger anti-apoptotic ability and is less prone to aging. Therefore, the fibroblast cultured by the fibroblast culture solution adopting the design disclosed by the invention can be used for producing cell engineering products with higher quality and higher efficiency by being applied to production practices.

Description

[0001] technical field [0002] The invention relates to the technical field of animal cell culture, in particular to a fibroblast culture fluid. Background technique [0003] Cells are the basic structural and functional units of organisms. In today's society where life sciences are rapidly developing, major scientific advances such as animal cloning, stem cells, mammary gland bioreactor transgenic animals, artificial tissues and organs are inseparable from the platform established by cell engineering. . Cell engineering is a comprehensive bioengineering in which humans obtain reconstructed cells, tissues, organs, and even individuals through experimental operations at the cell, subcellular, or tissue level according to a certain design plan, thereby cultivating and creating excellent varieties or products. The main technical fields involved in cell engineering include cell culture, cell fusion, cell disassembly, chromosome manipulation and gene transfer. Cell engineering...

Claims

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Application Information

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IPC IPC(8): C12N5/071
Inventor 曹鸿国章孝荣蒲勇杨盼张运海刘亚陶勇方富贵李运生
Owner ANHUI AGRICULTURAL UNIVERSITY
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