Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera

Abstract

The invention discloses a darkroom used between an installing seat of a sequencing chip and a CCD (Charge Coupled Device) camera. A ring-shaped groove surrounded outside the sequencing chip is arranged on the installing seat of the sequencing chip; the front end of the CCD camera is connected with a connecting flange; and the front end of the connecting flange is provided with a convex ring for being inserted into the ring-shaped groove and being matched with the ring-shaped groove in a sealing mode. According to the darkroom used between the installing seat of the sequencing chip and the CCD camera, which is disclosed by the invention, by the matching of the ring-shaped groove and the convex ring, a closed darkroom environment is formed between the CCD camera and the installing seat of the sequencing chip and the external optical pollution is prevented, so that the background noise is reduced and a high SNR (Signal to Noise Ratio) sequencing signal is ensured to be acquired.

Description

technical field [0001] The invention relates to the technical field of DNA sequencing, in particular to a darkroom between a mounting seat of a sequencing chip and a CCD camera, forming a light-tight darkroom environment and ensuring the acquisition of sequencing signals with a high signal-to-noise ratio. Background technique [0002] Pyrosequencing technology is an enzyme cascade chemiluminescent reaction in the same reaction system catalyzed by four enzymes. The principle of pyrosequencing technology is: after the primer anneals to the template DNA, the four enzymes DNA polymerase (DNA polymerase), ATP sulfurylase (ATP sulfurylase), luciferase (luciferase) and adenosine triphosphate (Apyrase) Under the synergistic effect of the primer, the polymerization of each dNTP on the primer is coupled with the release of an optical signal, and the purpose of real-time determination of DNA sequence is achieved by detecting the release and intensity of light. The reaction system of p...

AI Technical Summary

Problems solved by technology

[0006] In the prior art, the front end of the CCD camera is directly attached to the mounting seat of the sequencing chip on the reaction chamber, so that the optical fiber panel of the CCD camera is directly attached to the sequencing chip to obtain the optical signal of the sequencing reaction. The disadvantage of the prior art is that the CCD The optical fiber panel of the camera and the sequencing chip are easily interfered by visible light, which affects the normal progress of sequencing

Images