Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera

A sequencing chip and mounting base technology, applied in the field of DNA sequencing, can solve the problems affecting the normal progress of sequencing and the interference of visible light between optical fiber panels and sequencing chips, and achieve the effects of preventing optical pollution, high signal-to-noise ratio, and reducing background noise.

Inactive Publication Date: 2012-10-03
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION +1
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Problems solved by technology

[0006] In the prior art, the front end of the CCD camera is directly attached to the mounting seat of the sequencing chip on the reaction chamber, so that the optical fiber panel of the CCD camera is directly attached to the sequencing chip to obtai

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  • Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera
  • Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera

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[0019] The present invention will be further described in detail below with reference to the drawings and specific embodiments, but it is not intended to limit the present invention.

[0020] figure 1 , figure 2 Shows the structure of the connecting flange of the dark room between the mounting seat of the sequencing chip and the CCD camera of the present invention; image 3 Shows the structure diagram of the connecting flange installed on the CCD camera. Such as figure 1 with figure 2 As shown, there is an annular convex ring 3 at the front end of the connecting flange 2. Such as image 3 As shown, the connecting flange 2 is installed at the front end of the CCD camera 1, and is connected with the CCD camera 1 by bolts. The optical fiber panel 4 of the CCD camera 1 is located in the area enclosed by the convex ring 3, that is, the convex ring 3 extends out of the connecting flange 2 surrounds the outside of the fiber optic panel 4.

[0021] Figure 4 , Figure 5 It shows the str...

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Abstract

The invention discloses a darkroom used between an installing seat of a sequencing chip and a CCD (Charge Coupled Device) camera. A ring-shaped groove surrounded outside the sequencing chip is arranged on the installing seat of the sequencing chip; the front end of the CCD camera is connected with a connecting flange; and the front end of the connecting flange is provided with a convex ring for being inserted into the ring-shaped groove and being matched with the ring-shaped groove in a sealing mode. According to the darkroom used between the installing seat of the sequencing chip and the CCD camera, which is disclosed by the invention, by the matching of the ring-shaped groove and the convex ring, a closed darkroom environment is formed between the CCD camera and the installing seat of the sequencing chip and the external optical pollution is prevented, so that the background noise is reduced and a high SNR (Signal to Noise Ratio) sequencing signal is ensured to be acquired.

Description

technical field [0001] The invention relates to the technical field of DNA sequencing, in particular to a darkroom between a mounting seat of a sequencing chip and a CCD camera, forming a light-tight darkroom environment and ensuring the acquisition of sequencing signals with a high signal-to-noise ratio. Background technique [0002] Pyrosequencing technology is an enzyme cascade chemiluminescent reaction in the same reaction system catalyzed by four enzymes. The principle of pyrosequencing technology is: after the primer anneals to the template DNA, the four enzymes DNA polymerase (DNA polymerase), ATP sulfurylase (ATP sulfurylase), luciferase (luciferase) and adenosine triphosphate (Apyrase) Under the synergistic effect of the primer, the polymerization of each dNTP on the primer is coupled with the release of an optical signal, and the purpose of real-time determination of DNA sequence is achieved by detecting the release and intensity of light. The reaction system of p...

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Application Information

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IPC IPC(8): C12M1/00
Inventor 任鲁风王绪敏李运涛周晓光袁丽娜冯玉臣秦奕韩伟静谷岚滕明静俞育德于军
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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