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Yeast strain of high-yield cellulase and screening method of yeast strain

A cellulase and yeast strain technology, applied in the field of microorganisms, can solve the problems of low enzyme activity and low production of cellulase

Active Publication Date: 2014-07-02
HAINAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Cellulase produced by non-artificially modified yeast has more advantages in genetic performance and safety, but current research results show that general yeast rarely or does not produce cellulase. Foreign reports have screened out related yeasts from the tropics. Plant foliar yeast strains produce cellulase, but with low enzyme activity

Method used

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  • Yeast strain of high-yield cellulase and screening method of yeast strain
  • Yeast strain of high-yield cellulase and screening method of yeast strain
  • Yeast strain of high-yield cellulase and screening method of yeast strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Sampling: Sampling in the coconut plant that uses shallow plate fermentation to produce bacterial cellulose gel, select those cellulose gel membranes that are degraded by microbial contamination, and use sterilized filter paper to dip the culture solution in the contaminated area , into a sterile empty test tube, and plugged with a cotton stopper for later use.

[0045] Cultivation: Put the sampled filter paper into liquid culture medium for cultivation, the cultivation temperature is 25° C., and the cultivation time is 5 days. The formula of the above liquid medium is: 2 grams of white sugar as a carbon source, 0.05 grams of urea as a nitrogen source, dissolved in 100 ml of fresh coconut water, and the pH value is adjusted to 5.5. The medium was sterilized at 100°C for 5 minutes.

[0046] Separation: The microorganisms are separated by the plate coating method, and the yeast colonies are numbered for future use. The solid medium used in the plate coating method is fo...

Embodiment 2

[0052] Sampling: Sampling was carried out in a coconut plant that produced bacterial cellulose gel by shallow plate fermentation, and the cellulose gel film degraded by microbial contamination was selected, and the culture solution in the contaminated area was dipped with a sterilized filter paper strip. Put it into a sterile empty test tube and plug it with a cotton stopper for later use.

[0053] Cultivation: Put the sampled filter paper into liquid culture medium for cultivation, the cultivation temperature is 35° C., and the cultivation time is 2 days. The formula of the above liquid medium is: 5 grams of fructose syrup as a carbon source, 0.1 gram of ammonium sulfate or urea as a nitrogen source, dissolved in 150 ml of fresh coconut water, and adjusted to a pH value of 5.0. The medium was sterilized at 100°C for 10 minutes.

[0054] Separation: Since there are many types of microorganisms in the sampling, the microorganisms in the above-mentioned culture solution are sep...

Embodiment 3

[0060] Sampling: Sampling in the coconut plant that uses shallow plate fermentation to produce bacterial cellulose gel, select those cellulose gel membranes that are degraded by microbial contamination, and use sterilized filter paper to dip the culture solution in the contaminated area , into a sterile empty test tube, and plugged with a cotton stopper for later use.

[0061] Cultivation: put the sampled filter paper into liquid culture medium for cultivation, the cultivation temperature is 32° C., and the cultivation time is 3 days. The formula of the above liquid medium is: 5 grams of white sugar as a carbon source, 0.1 gram of ammonium sulfate as a nitrogen source, dissolved with 150 ml of fresh coconut water, and adjusted to a pH value of 5.0. The medium was sterilized at 100°C for 5 minutes.

[0062] Separation: Since there are many types of microorganisms in the sampling, the microorganisms in the above-mentioned culture solution are separated by the flat plate coating...

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Abstract

The invention relates to the field of microorganisms, in particular to a yeast strain of high-yield cellulase and a screening method of the yeast strain. The collection number of the yeast strain of high-yield cellulase is CGMCC No. 6057; the yeast strain is easy to culture, high in production safety and better in genetic stability, and the metabolite cellulase of the yeast strain is easy to separate and purify; and the enzyme activity of the metabolite cellulase of the yeast strain is high and has higher application value. The invention also provides a screening method of the yeast strain of the high-yield cellulase. The screening method comprises the following steps of: sampling cellulose gel which is polluted by the microorganisms and is degraded from the bacterial cellulose gel in a shallow tray fermentation production mode; and then separating and purifying the yeast strain capable of producing the cellulase.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a yeast strain with high cellulase production and a screening method thereof. Background technique [0002] Cellulose is the main component of plant cell walls, accounting for about 1 / 3 to 1 / 2 of the dry weight of plants. It is the most widely distributed, most abundant, and most produced organic compound on the earth, and is the largest renewable resource in nature. Therefore, the utilization and transformation of cellulose is of great significance to solve the current world energy crisis, food shortage and environmental pollution. [0003] Cellulose degradation mainly includes acid degradation and enzymatic degradation. Acid degradation requires a certain pressure and temperature, which requires high equipment. The production process will generate a large amount of waste, which is difficult to meet the requirements of environmental protection. Enzymatic degradation reaction condi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/19C12Q1/04C12R1/645
Inventor 向东王锡彬钟春燕王志国李斌冯爱国
Owner HAINAN UNIV
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