Mesenchymal stem cell nutrient solution

Abstract

The invention provides a serum-free mesenchymal stem cell nutrient solution, which is simple in formula, low in cost and high in safety. The serum-free mesenchymal stem cell nutrient solution comprises a serum-free animal cell culture medium, a B27 serum-free additive, bFGFs (basic fibroblast growth factors) and EGFs (epidermal growth factors), wherein the nutrient solution also comprises fetuin and antibiotics. The nutrient solution does not contain animal serum, has higher safety and definite formula components, and is suitable for clinical treatment. Meanwhile, according to the serum-free mesenchymal stem cell nutrient solution, human fat stem cells can be normally attached to the surface of a culture vessel and grow, the antibiotics are added into the formula components, and the formula is simpler compared with that of the general serum-free nutrient solution. For the fat stem cells cultured according to the conventional formula, the cells began to differentiate and slowly grow after two generations. Compared with the fat stem cells cultured according to the conventional formula, the fat stem cells cultured according to the formula of the serum-free mesenchymal stem cell nutrient solution do not have the phenomena of differentiation and slow growth after being tested, so that the serum-free mesenchymal stem cell nutrient solution is extremely suitable for the growth of the fat stem cells and gum stem cells.

Description

technical field

[0001] The invention relates to the culture technology of mesenchymal stem cells. Background technique

[0002] Mesenchymal stem cells are cells with high differentiation potential, which are different from ordinary somatic cells and have the ability to differentiate into various body tissues, such as bones, skin, heart, nerves, etc. Its application in regenerative medicine has been increasing in recent years. The source of cells is mainly extracted from human tissues and cultivated in vitro to obtain the required number of stem cells for treatment. However, the traditional culture medium used for in vitro proliferation of cells also contains 5% to 20% of animal serum, if the serum used contains incurable viruses or pathogens (such as mad cow disease prions), patients receiving cell therapy will be infected, and the consequences will be very serious. Since the main ingredient that stimulates cell growth in the culture medium is serum, scientists try to repla...