Mesenchymal stem cell nutrient solution
A cell culture and stem cell technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of cell contamination, high cost, and low risk of cell contamination, and achieve simple formula and high safety sexual effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0017] Wash 20cc adipose tissue 3 times with phosphate buffered saline, add collagenase (Type II, 50-100U / ml) to digest the tissue for one hour, centrifuge the digest and wash the resulting cells 3 times with phosphate buffered saline; mix the cells 7ml of culture solution was placed in a T-75 cell culture flask for cultivation. The components of the culture solution in this example were: alpha-MEM medium, B27 serum-free additive with a volume ratio of 1%, bFGF at 10 ng / ml, bFGF at 10 ng / ml EGF, 0.5mg / ml fetuin, 100units / ml each of penicillin and streptomycin, use the above culture medium, replace the culture medium once every 3 days, and detect when the cells grow to a T-75 cell culture flask. No less than one million adipose-derived mesenchymal stem cells were cultured.
Embodiment 2
[0019] Use 70% ethanol solution by volume to disinfect the oral cavity of the human body, and then use a 2mm diameter biopsy instrument to extract tissue samples (not less than 2mmx2mmx2mm) from the human gingiva, wash them with phosphate buffer for 3 times, and add collagenase (Type II, 50 -100U / ml) to digest the tissue for one hour, centrifuge the digest and wash the resulting cells 3 times with phosphate buffered saline; mix the gingival tissue cells with 10ml of culture solution and put them in a T-75 cell culture bottle for cultivation. The cultivation of the present embodiment The liquid components are: DMEM / F12 serum-free medium, B27 serum-free supplement with a volume ratio of 3%, 50ng / ml bFGF, 50ng / ml EGF, 2mg / ml fetuin, 150units / ml of penicillin and Streptomycin, using the above culture medium, replace the culture medium once every 3 days, when the cells grow to a T-75 cell culture flask, it is detected that no less than one million gingival tissue mesenchymal stem ce...
Embodiment 3
[0022] Thaw adipose-derived mesenchymal stem cells (not less than 400,000 cells) to 37 degrees, wash the obtained cells twice with phosphate buffer, mix the cells with 20ml of culture medium and put them in a T-150 cell culture bottle for cultivation. This implementation The culture solution components of the example are: alpha-MEM medium, B27 serum-free supplement with a volume ratio of 2%, bFGF at 20ng / ml, EGF at 20ng / ml, fetuin at 1mg / ml, penicillin at 100units / ml each And streptomycin, using the above culture medium, replace the culture medium once every 3 days, when the cells grow to a T-75 cell culture flask, it is detected that no less than 3 million adipose-derived mesenchymal stem cells have been cultured.
[0023] Compared with the culture of adipose-derived mesenchymal stem cells using the serum-free medium formula of Dromard C et al., 2011, the cells will grow in suspension in the culture flask, while the mesenchymal stem cells of this example can be attached to the...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com