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Lincomycin assay kit and method for making the same

A detection kit, the technology of lincomycin, applied in the field of determination of biological immunological methods, can solve the problems of complicated detection, time-consuming, expensive instruments, etc., and achieve the effect of simple operation, rapid response and convenient use

Inactive Publication Date: 2012-10-10
库尔医疗技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have high sensitivity and accurate results, but have the disadvantages of requiring expensive instruments, cumbersome and time-consuming detection, and the detection personnel need certain professional training.

Method used

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  • Lincomycin assay kit and method for making the same
  • Lincomycin assay kit and method for making the same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the preparation of lincomycin detection kit

[0039] 1. Preparation of Lincomycin-Conjugated Bovine Serum Albumin

[0040] Coupling lincomycin with bovine serum albumin, and synthesizing lincomycin-conjugated bovine serum albumin as immunogen and coating.

[0041] 2. Preparation of lincomycin monoclonal antibody

[0042] Lincomycin monoclonal antibody was prepared by immunizing BALB / C mice with lincomycin-conjugated bovine serum albumin.

[0043] 3. Preparation of colloidal gold

[0044] Take 0.01% chloroauric acid aqueous solution, heat to boil. Quickly add 1ml of 1% trisodium citrate aqueous solution as needed, continue to boil for about 5min, orange-red appears. The colloidal gold particles thus produced have a size of 20-40 nm.

[0045] 4. Preparation of Colloidal Gold Pads

[0046] Take 5ml of colloidal gold solution with a particle size of 20-40nm, and use 0.2mol / L K 2 CO 3 Adjust the pH value of the colloidal gold solution to 8.0, and place ...

Embodiment 2

[0056] Embodiment 2: the detection of lincomycin detection kit

[0057] 1. Sample handling

[0058] Serum: extract the serum, centrifuge or stand still and take the clear supernatant for use;

[0059] Urine: Use urine to test directly. If the urine is visibly turbid, it needs to be centrifuged, filtered, or the supernatant should be tested after it settles;

[0060] Milk: centrifuge at 3000r / min at 4°C for 15min, remove the upper layer of fat and then detect it.

[0061] Honey and eggs: Use 0.01mol / L PBS with pH 7.5 to make a 1:2 suspension of the sample to be tested, shake for 10 minutes, centrifuge at 3000r / min for 15 minutes, and take the supernatant for testing.

[0062] Tissue samples: Take 5-10g of tissue samples and mash them, add 20-30ml (0.01mol / L, pH 7.5) PBS, shake for 10min, react in a 37°C incubator for 30min, then bathe in water for 10min, at 4°C, 3000r / min Centrifuge for 15 minutes, remove the upper layer of fat, and take the supernatant for detection.

[00...

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Abstract

A lincomycin assay kit and a method for making the same are provided in the invention, and relate to a field of determination technologies for biology immunity methods. The assay kit is composed of a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3), a sample suction pad (4) and a PVC support plate (5), wherein the sample pad, the colloidal gold pad, the nitrocellulose membrane and the sample suction pad are consecutively adhered to the PVC support plate in turn. The colloidal gold pad is a lincomycin monoclonal antibody polyester film which is labelled by the colloidal gold, and the nitrocellulose membrane, in order, is coated with a lincomycin coupling carrier protein as a test line (T line) and a goat-anti-mouse IgG polyclonal antibody as a quality control line (C line). As lincomycin that may be contained in a detected sample and the lincomycin coupling carrier protein coated on the nitrocellulose membrane compete to combine with the colloidal gold-labelled lincomycin monoclonal antibody, a colloidal gold immunochromatography technique is used to detect whether or not the sample contains lincomycin.

Description

technical field [0001] The invention relates to the technical field of determination of biological immunological methods, in particular to a lincomycin detection kit made by colloidal gold immunochromatography technology and a preparation method thereof. Background technique [0002] Lincomycin, the English name is Lincomycin. It acts on the 50S subunit of the ribosome of sensitive bacteria to prevent the extension of the pus chain, thereby inhibiting the protein synthesis of bacterial cells. Lincomycin is generally a bacteriostatic agent, but at high concentrations, it also has a bactericidal effect on highly sensitive bacteria. In addition to being mainly used clinically for various infections caused by sensitive bacteria, lincomycin is also widely used in animal feeding and treatment of animal diseases. Although lincomycin is not very toxic, long-term use by animals can also be potentially harmful to humans and animals. Therefore, various countries have restricted the c...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/532
Inventor 陈立柱杨利李峰
Owner 库尔医疗技术(北京)有限公司
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