Cefradine molecule imprinted membrane preparation method and application

A molecularly imprinted membrane, cefradine technology, used in chemical instruments and methods, membrane technology, semi-permeable membrane separation and other directions

Inactive Publication Date: 2012-10-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the preparation of cephradine molecularly imprinted membrane a

Method used

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  • Cefradine molecule imprinted membrane preparation method and application
  • Cefradine molecule imprinted membrane preparation method and application
  • Cefradine molecule imprinted membrane preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0068] Embodiment 1, a kind of preparation method of cephradine molecularly imprinted membrane, carries out following steps successively:

[0069] 1) Preparation of polyacrylonitrile-based membrane by phase inversion method:

[0070] Weigh 5g of polyacrylonitrile and dry it under vacuum at 60°C for 24 hours, add it together with 1g of lithium chloride into 26.5ml of N-methylpyrrolidone, and magnetically stir at 60°C overnight (12 hours), so that polyacrylonitrile and chlorinated Lithium is fully dissolved.

[0071] The obtained solution was filtered and defoamed, and stood at 60°C for 2 h, and scraped the film on the glass plate with a 200 μm spatula to control the thickness of the film to 200 μm. After staying in the air for a certain period of time (1–5 minutes), the glass plate was Immerse in deionized water at a certain temperature (20-30 °C). After the membrane was formed, it was soaked in deionized water at room temperature for 36 h, and the deionized water was changed...

Embodiment 2

[0078] Embodiment 2, a kind of method for measuring the concentration of cephradine in the liquid test sample, carries out following steps successively:

[0079] 1) To draw a standard curve, follow the steps below:

[0080] ①. Prepare fluorescein with pH 5.8 phosphate buffer to a concentration of 1×10 -6 mol / L fluorescein solution;

[0081] ②. Add a certain amount (or not) of cephradine solution (with deionized water as solvent) to each of the 7 volumetric flasks, add 2ml of fluorescein solution to each volumetric flask, and then dilute to 10ml with deionized water ; Thereby be mixed with the cephradine concentration and be respectively a series of cephradine standard solutions of 0,0.5,1,2,3,4,5mg / L; Carry out following steps 3. immediately after preparation finishes.

[0082] 3., adopt fluorescence method, at excitation wavelength 484nm, emission wavelength 512nm place, detect above-mentioned series of cephradine standard solution fluorescence value, take cephradine concen...

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Abstract

The invention discloses a preparation method of a cefradine molecule imprinted membrane, which comprises the following steps: 1) using a phase inversion method to prepare a polyacrylonitrile basal film; 2) preparing the cefradine molecule imprinted membrane: taking cefradine as a template molecule, taking methacrylic acid as a functional monomer, taking ethylene glycol dimethacrylate as a cross-linking agent; taking azodiisobutyronitrile as an initiator; using cefradine, tetrabutylammonium hydroxide and methanol to prepare a filtrate; adding methacrylic acid, ethylene glycol dimethacrylate and azodiisobutyronitrile in the filtrate, uniformly stirring to obtain a membrane liquid; putting the obtained polyacrylonitrile basal film in the membrane liquid for immersing, then performing polymerization and elution in order to obtain the cefradine molecule imprinted membrane. The cefradine molecule imprinted membrane can used for determining the concentration of cefradine in a liquid state sample to be measured. The invention also provides a method for determining the concentration of the liquid state sample to be measured in cefradine.

Description

technical field [0001] The invention relates to the preparation and application of a cephradine molecularly imprinted membrane. Background technique [0002] Cephradine (Cephrading, Velosef), also known as cephalosporin VI and cephalosporin VI, is the first-generation semi-synthetic cephalosporin with a wide range of antibacterial properties, good oral absorption, high blood concentration, and is characterized by resistance to β-lactamase , It has rapid and reliable bactericidal effect on drug-resistant Staphylococcus aureus and other bacilli resistant to spectrum antibiotics. It is mainly excreted in the urine in the original form, and the concentration in the urine is relatively high. Clinically, it is mainly used for infections of the respiratory tract, urinary tract, skin and soft tissues. The 2005 edition of Chinese Pharmacopoeia adopts HPLC to determine the content of cephradine capsules, and the assay methods reported in the literature have spectrophotometry, chemil...

Claims

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Application Information

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IPC IPC(8): C08J9/26C08J7/16C08L33/20B01D71/78B01D67/00G01N21/64
Inventor 沈生荣于海宁曾思敏
Owner ZHEJIANG UNIV
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