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Biochemical oxygen demand detection method

A biochemical oxygen demand and detection method technology, applied in the field of biochemical oxygen demand detection, can solve problems such as low accuracy of BOD value, and achieve the effects of improving accuracy, increasing biodegradation efficiency and sufficient contact

Inactive Publication Date: 2015-05-20
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the biodegraded products of organic matter are often not completely carbon dioxide, and the oxygen consumption is not directly related to the amount of carbon dioxide produced, but depends on the type of organic matter, so the BOD value obtained by this method is less accurate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] according to figure 1Schematic diagram of the preparation of the microbial membrane reactor is shown. The thermostat 10 is switched on and the temperature is adjusted to 37°C. Inject 500 mL of activated sludge water sample into sample cup 1. A glass reactor 7 with a length of 220.0 cm and an inner diameter of 2.4 mm was placed in a constant temperature water bath. Turn on the switch of the controller 11, and set its control mode for the two-position three-way solenoid valve 3 to work for 1 second every 1 second. The rotation speed of the peristaltic pump 6 was set to 9 rpm, and the measured actual flow rate of the water sample was 1.0 mL / min. At this time, the activated sludge and air in the sample cup 1 are controlled by the peristaltic pump 6 through the first sampling tube 2 and the air intake tube 4 respectively, and alternately pass through the second sampling tube 5 every 1 second to reach the reactor 7 . At the same time, the activated sludge water sample is ...

Embodiment 2

[0092] Turn on the constant temperature water bath 20 and adjust the temperature to 37°C. The microbial membrane reactor obtained in Example 1 was cleaned for 10 minutes according to the cleaning steps and parameters of the microbial membrane reactor described in Example 1. Dissolve 150mg of glucose and 150mg of glutamic acid in tap water and set the volume to 100mL to obtain a biochemical oxygen demand concentration of 1980.0mg O 2 / L of the mother liquor of the GGA solution. The mother liquor is diluted with tap water to obtain the biochemical oxygen demand concentration as 10.0mg O 2 / L, 20.0mg O 2 / L, 50.0mg O 2 / L, 100.0mgO 2 / L, 200.0mg O 2 / L, 400.0mg O 2 / L, 600.0mg O 2 / L, 800.0mg O 2 / L and 1000.0mg O 2 / L of GGA solution. In the present invention, at first the biochemical oxygen demand concentration is 10.0mg O 2 / L of the GGA solution was injected into the sample cup 1, and the measurement of the GGA solution was completed according to the GGA measuremen...

Embodiment 3

[0095] Turn on the constant temperature water bath 20 and adjust the temperature to 37°C. According to the microbial membrane reactor cleaning and GGA measurement steps and parameters described in Example 2, the sewage treatment plant sample, the beverage factory water sample, the milk factory water sample, the corn deep processing plant water sample, the medical wastewater sample, the cake factory water sample and the kitchen wastewater Samples were tested for biochemical oxygen demand. At the same time, the national standard BOD is adopted 5 Method Determination of BOD on the above samples 5 .

[0096] According to the GGA standard curve described in Example 2, the present invention calculates the rapid biochemical oxygen demand value of the corresponding sample from the obtained chemical oxygen demand difference. Take the calculated rapid biochemical oxygen demand concentration value of the sample as the ordinate, and detect the BOD obtained by the national standard bioc...

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Abstract

The invention provides a biochemical oxygen demand detection method which comprises the following steps: a) subjecting a water sample containing microbes to microbiological culture so as to obtain a microbial membrane; b) allowing a target water sample and air to pass through the microbial membrane obtained in step a ) to detect chemical oxygen demand of the target water sample which has been degraded by the microbes; c) calculating difference of the chemical oxygen demands of the target water sample based on initial water chemical oxygen demand and the chemical oxygen demand obtained step b) of the target water sample; and d) calculating biochemical oxygen demand of the target water sample according to a predetermined standard curve and the chemical oxygen demand of the target water sample obtained in step c), wherein the standard curve in step d) is a relation curve between the chemical oxygen demand difference and the biochemical oxygen demand of the target water sample. According to the invention, the key technology of treating sewage with a biological membrane is used to provide sufficient air for degradation of organic compounds, which enables efficiency of non-selective degradation of organic matters by microbes to be enhanced, thereby improving the accuracy of detection results.

Description

technical field [0001] The invention relates to the technical field of environmental monitoring, in particular to a method for detecting biochemical oxygen demand. Background technique [0002] Biochemical oxygen demand (BOD) refers to the amount of dissolved oxygen consumed by microorganisms to decompose certain oxidizable substances in water, especially organic substances. If the time for biological oxidation is five days, it is called five-day biochemical oxygen demand. (BOD 5 ). Biochemical oxygen demand is an important indicator for analyzing the content of organic pollutants in water bodies and one of the most important parameters in routine monitoring of water quality. The higher the value, the more organic pollutants in the water and the more serious the pollution. [0003] At present, the dilution and inoculation method is generally used in the determination of biochemical oxygen demand in the world, also known as BOD. 5 However, this method is time-consuming, he...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/18
Inventor 刘长宇董绍俊赵惠军
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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