Method for enhancing precipitation effect of acid precipitated proteins

A technology of protein sedimentation, alkali extraction and acid precipitation, which is applied in the field of protein processing, can solve the problems of low protein yield and incomplete sedimentation, and achieve the effect of increasing protein yield, prolonging residence time and improving separation effect

Inactive Publication Date: 2012-10-31
谷神生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The disadvantage of this method is that in the alkaline extraction process (including the extraction of each section) of protein separation by the alkaline extraction and acid precipitation process, a large amount of foam will be generated due to stirring and homogenization, although some sections do not have visible bubbles. Foam, but the protein liquid has formed a protein and air emulsification system, so that when the pH value of the acid precipitation section is adjusted to about 4.5, the protein is coagulated but does not settle or does not settle completely, resulting in a low protein yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Using low-temperature defatted soybean meal as raw material, conduct the first extraction, add 0.2% defoamer to the first extraction tank; conduct second extraction on the separated bean dregs, add defoamer to the second extraction tank 0.2% agent; mix the protein material liquid separated by two extractions, add 0.2% defoamer to the extract mixing tank; carry out acid precipitation on the extract, add 0.2% defoamer to the acid sink tank; The sinking feed liquid enters the aging tank and stays for 30 minutes in order to improve the precipitation effect of the protein curd; the protein curd is separated by a centrifuge, and enters the neutralization tank for neutralization, and finally after sterilization, flash evaporation , drying and separating to obtain soybean protein isolate.

Embodiment 2

[0011] Example 2: Using low-temperature defatted soybean meal as raw material, conduct the first extraction, add 0.8% defoamer to the first extraction tank; conduct second extraction on the separated bean dregs, add defoamer to the second extraction tank 0.6% agent; mix the protein material liquid separated by two extractions, add 1.2% defoamer to the extract mixing tank; carry out acid precipitation on the extract, add 1% defoamer to the acid sink tank; The sinking feed liquid enters the aging tank and stays for 20 minutes in order to improve the precipitation effect of the protein curd; the protein curd is separated by a centrifuge, and enters the neutralization tank for neutralization, and finally after sterilization, flash evaporation , drying and separating to obtain soybean protein isolate.

Embodiment 3

[0012] Example 3: Using low-temperature defatted soybean meal as raw material, conduct the first extraction, add 2% defoamer to the first extraction tank; conduct second extraction on the separated bean dregs, add defoamer to the second extraction tank Mix the protein material liquid separated by two extractions, add 2% defoamer to the extract mixing tank; carry out acid precipitation on the extract, add 2% defoamer to the acid precipitation tank; The sinking feed liquid enters the aging tank and stays for 10 minutes in order to improve the precipitation effect of the protein curd; the protein curd is separated by a centrifuge, and enters the neutralization tank for neutralization, and finally after sterilization, flash evaporation , drying and separating to obtain soybean protein isolate.

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PUM

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Abstract

The invention discloses a method for enhancing precipitation effect of acid precipitated proteins, which is implemented in a way that: in the protein extraction process by alkali extraction and acid precipitation, a defoaming agent is added to the extraction and acid precipitation sections to ensure that no protein or air emulsion system is formed in the extraction process; and a pipe bundle or ageing can is added to the rear part of the acid precipitation tank in the acid precipitation process, so that the protein liquid in the acid precipitation tank enters the pipe bundle or ageing can before separation and stays for 10-30 minutes, thereby prolonging the retention time of the protein liquid at the acid precipitation section. The invention effectively prevents proteins from floating in the acid precipitation process, enhances the separating effect, prolongs the retention time of the protein liquid at the acid precipitation section, and directly enhances the protein yield.

Description

technical field [0001] The invention relates to the field of protein processing, and relates to a method for improving the protein sedimentation effect and increasing the yield in the protein extraction technology of the alkali extraction and acid precipitation method. Background technique [0002] Alkaline extraction and acid precipitation method to extract protein isolates from various grains has been widely used as a protein extraction method. The specific principle is: most of the proteins can be dissolved in dilute alkaline solution. The centrifugation method can remove the insoluble substances (mainly polysaccharides and some residual proteins) in the raw materials, and then use acid to adjust the pH value of the leachate to about 4.5, the proteins will coagulate and precipitate due to the isoelectric point state, and can be obtained by separation. The protein precipitate is isolated protein after neutralization, drying and other processes. [0003] The disadvantage o...

Claims

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Application Information

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IPC IPC(8): C07K1/30
Inventor 李世伟王建忠高建勇姚琪
Owner 谷神生物科技集团有限公司
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