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Two-generation in one year reproduction method for konjac cross breeding

A hybrid breeding and hybrid seed technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as slow breeding process, and achieve the effect of improving reproduction coefficient, speeding up breeding pace, and improving breeding efficiency

Inactive Publication Date: 2012-11-07
云南省农业科学院生物技术与种质资源研究所 +2
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome the existing konjac breeding process after hybridization to form seeds, it takes the second year to carry out F 1 Planting of plants and single plant selection lead to slow defects and deficiencies in the breeding process. The purpose of the present invention is to shorten the breeding process of new konjac varieties and improve the breeding efficiency of new varieties

Method used

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  • Two-generation in one year reproduction method for konjac cross breeding

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Experimental program
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Effect test

Embodiment 1

[0013] The konjac used is flower konjac, which is pollinated and hybridized in different plants. When the seeds are mature, the berry peel outside the seeds is orange-red.

[0014] (1) the konjac is hybridized by conventional heterogeneous intraspecific pollination to form a hybrid, and when 1 / 3 of the berry pericarp outside the hybrid seed formed in the same year changes from green to orange red, the berry pericarp is peeled off to collect the seeds;

[0015] (2) Tissue culture the seeds collected in step (1) with the existing plant tissue culture method, and in the same year of step (1), plant rooted seedlings on the seedbed according to the conventional method, and perform seedbed management and single plant filter. Specifically, it can be carried out as follows:

[0016] Disinfect the seeds collected in step (1) with 70% alcohol surface for 2 seconds → sterilize with 0.1% mercuric chloride for 10 minutes → rinse with sterile water 3 times → inoculate under sterile condit...

Embodiment 7

[0023] When the seeds of Example 7 (Control 1) and Example 8 (Control 2) were induced and cultivated, the seedling growth rate was 0, so the follow-up test described in Example 1 could not be carried out, and the second-generation multiplication per year could not be realized. When all the berry peels of the seeds are orange-red or all blue, the fruit is ripe, and the seeds in it are mature seeds, which have entered the physiological dormancy period, and the seedling rate of using mature seeds to induce it is 0.

[0024] Above-mentioned experiment shows: will realize konjac crossbreeding one year two generation add generation propagation, shorten breeding process, the key is to select suitable period to collect seed, the seed that makes collection can be induced to differentiate into seedling, and the present invention selects in the year conventional crossbreeding to form The period when 1 / 3~2 / 3 of the berry pericarp outside the hybrid seeds changes from green to orange-red or...

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Abstract

The invention relates to a two-generation in one year reproduction method for konjac cross breeding and belongs to the technical field of agricultural breeding. The method is characterized in that when 1 / 3-2 / 3 of the berry peel outside cross breeding seeds formed in the current year through konjac conventional cross breeding is turned from green into reddish orange or blue, the berry peel is stripped to obtain the seeds, the seeds are cultivated through an existing plant tissue cultivation method, seedlings with roots are planted on a seedbed in the same year, the seedlings grow more than three months, and single plant screening is performed. The seeds obtained by the method are induced and cultivated, seedling emergence percentage of the seeds reaches 65%-90%, and the seedling emergence percentage of mature seeds through induction and cultivation is 0. The two-generation in one year reproduction method for the konjac cross breeding achieves the purpose of two-generation in one year reproduction, improves reproduction coefficient, accelerates breeding step, and improves breeding efficiency.

Description

technical field [0001] The invention relates to a konjac hybrid breeding method with two generations per year and belongs to the technical field of agricultural breeding. Background technique [0002] Konjac ( Amorphophallus rivieri Durieu) is a perennial herbaceous plant of the genus Konjac in the Araceae family. Its species is determined mainly by asexual reproduction. Due to long-term asexual reproduction, the species is mixed, the species is degraded, the corm expansion rate is reduced, and the yield is reduced. The existing Varieties can no longer adapt to the rapid development of konjac industry. Therefore, using the abundant konjac resources in Yunnan Province to carry out the breeding of fine varieties, and the breeding of fine varieties with high yield, stable yield, high quality and strong stress resistance are the guarantee for the sustainable development of this industry. In terms of crop breeding, genetic engineering, biotechnology, radiation and other high t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王玲马继琼尹桂芳孙道旺姜德有徐云孙涛陈燕萍蒋晓云
Owner 云南省农业科学院生物技术与种质资源研究所
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