Phellinus igniarius health-care beverage prepared through liquid submerged fermentation and preparation method thereof
A technology of submerged liquid fermentation and Porophora spp., which is applied in botany equipment and methods, food preparation, gardening, etc., can solve the problems of difficult artificial production, difficult commercial use in technical fields, health care and therapeutic effects Problems such as the shortage of fruiting bodies of the forobacteria, to achieve the effects of reduced production costs, controllable costs, and simple processes
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Embodiment 1
[0016] The following is a preferred embodiment of a method for preparing a Phylocarpus health drink by liquid submerged fermentation of the present invention. The preparation method of the present invention includes the following main steps:
[0017] A, first, insert Phylophorus bacterium into the culture fluid, the culture fluid of the present invention can be the various culture fluids suitable for the liquid culture of Phylophorus bacterium in the prior art, and at 18 ℃ Under the temperature with the vibration frequency of 300 times / min, shake and cultivate on the shaker for 72 hours to obtain the seed liquid; B, then, insert the seed liquid obtained in step A containing 8% barley malt, 6% by weight In the soybean liquid medium, the inoculum amount is 5%, and it is aerated and cultivated at a temperature of 27°C for 48 hours, and after biological fermentation, the fermented Ph. C, after the cell structure of the Phylophorus pyrilicum mycelia obtained in step B is broken, ex...
Embodiment 2
[0024] Another preferred example of the production method of the present invention will be provided as follows. The preparation method of the present embodiment includes: A. First, select the edible and medicinal fungus Phylophora bacterium, insert the Phylopodium bacterium into the culture solution, and use 200 times / min at a temperature of 28°C Oscillation frequency is shaken and cultivated on a shaker for 96 hours to obtain the seed liquid; B, the seed liquid obtained in step A is inserted into a liquid medium containing 12% barley malt by weight and 3% soybean by weight, and the inoculum size is 10%, and aerated at a temperature of 23°C for 72 hours, through biological fermentation, to obtain the fermented Phylophorus mycelium and Phylonotus fermented liquid; After breaking the cell tissue of the Phyloporus mycelia, extracting with water, the extraction ratio is 1:2, the extraction temperature is 90°C, and the extraction time is 3 hours, so as to obtain the Phylonotus myce...
Embodiment 3
[0026] Still another preferred example of the production method of the present invention will be provided as follows. The preparation method of this embodiment includes the following steps: A. Introduce Phylophorus bacterium into the culture solution, and vibrate and culture on a shaking table at a shaking frequency of 260 times / min at a temperature of 22°C for 80 hours to obtain bacteria Seed liquid; B, insert the bacterial seed liquid obtained in step A into the liquid culture medium containing 10% barley malt by weight and 5% soybean by weight, the inoculum size is 7%, and aerated culture at a temperature of 25°C After 60 hours, through biological fermentation, the fermented Phylonotus mycelium and the Phylonotus fermented liquid were obtained; Extract with water, the leaching ratio is 0.9:4, the leaching temperature is 93°C, and the leaching time is 2.5 hours, so as to obtain the Phylophora mycelium extract; After mixing the fermentation broth of the fungus and the myceli...
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