Method for extracting coffee polyphenols from coffee bean
A technology for coffee beans and coffee, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, antidote and other directions, can solve the problems of low yield of coffee polyphenols, low product purity, and no separation and purification, and achieve production costs. Low, high purity, easy to operate effect
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Embodiment 1
[0018] A method for extracting polyphenols from coffee beans, comprising the following steps:
[0019] (1) Extraction: After crushing 1Kg of coffee beans into coarse powder, reflux extraction with 10Kg of 70% ethanol, the extraction time is 2 hours, the extraction temperature is 70°C, and the number of extractions is 2 times. Soak for 2 hours and combine the extracts;
[0020] (2) Refining and purification: the extract is firstly filtered with a polyethylene microporous filter tube, and then finely filtered with a cellulose acetate filter membrane with a pore size of 0.45um to collect the filtrate;
[0021] (3) Concentration and drying: the ethanol is recovered from the filtrate until it has no alcohol smell, and then concentrated in vacuum to an extract with a relative density of 1.20, and then spray-dried.
Embodiment 2
[0023] A method for extracting polyphenols from coffee beans, comprising the following steps:
[0024] (1) Extraction: After crushing 1Kg of coffee beans into coarse powder, reflux extraction with 5Kg of 80% ethanol, the extraction time is 1 hour, the extraction temperature is 50°C, and the number of extractions is 3 times. Soak for 1 hour and combine the extracts;
[0025] (2) Refining and purification: the extract is firstly filtered with a polyethylene microporous filter tube, and then finely filtered with a cellulose acetate filter membrane with a pore size of 0.3um to collect the filtrate;
[0026] (3) Concentration and drying: the ethanol is recovered from the filtrate until it has no alcohol smell, then concentrated in vacuum to an extract with a relative density of 1.30, and dried under reduced pressure.
Embodiment 3
[0028] A method for extracting polyphenols from coffee beans, comprising the following steps:
[0029] (1) Extraction: After crushing 1Kg of coffee beans into coarse powder, reflux extraction with 25Kg of 60% ethanol. The extraction time is 3 hours, the extraction temperature is 80°C, and the number of extractions is 2 times. Before the first extraction, the temperature is 60°C Soak for 3 hours and combine the extracts;
[0030] (2) Refining and purification: the extract is firstly filtered with a polyethylene microporous filter tube, and then finely filtered with a cellulose acetate filter membrane with a pore size of 0.6um to collect the filtrate;
[0031] (3) Concentration and drying: the ethanol is recovered from the filtrate until it has no alcohol smell, and then concentrated in vacuum to an extract with a relative density of 1.20, and then spray-dried.
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