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Method for extracting and separating squill saponin C

A separation method and saponin technology, which is applied in the field of natural product separation, can solve problems such as squill saponin C that has not yet been seen, and achieve the effects of short cycle, good separation effect, and reduced sample loss

Inactive Publication Date: 2012-12-19
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no relevant literature and patent reports on the industrial extraction and purification method of squill saponin C in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Blu-ray flower bulbs are crushed, weighed 3kg, and added to a supercritical extraction kettle for supercritical CO 2 Extraction, extraction temperature is 60°C, extraction pressure is 25MPa, analysis temperature is 50°C, analysis pressure is 9MPa, CO 2 The flow rate is 20L / h, the oily substances are removed, the raw material residue is released, and 80% ethanol solution is added for microwave extraction for 3 times. The microwave extraction power is 800w, and the extract is obtained by filtration. The extract is added with 4% of the liquid volume (W / V) The activated carbon was decolorized at 70°C for 20 minutes, and the decolorized solution was obtained by filtration. The decolorized solution was added to a hollow cellulose ultrafiltration membrane system with a molecular weight cut-off of 3000 for ultrafiltration, and the permeate was added to a hollow cellulose nanofiltration membrane system with a molecular weight cut-off of 800. Concentrate and dry the retentate to ...

Embodiment 2

[0023] Blu-ray flower bulbs are crushed, weighed 3kg, and added to a supercritical extraction kettle for supercritical CO 2 Extraction, extraction temperature is 40°C, extraction pressure is 15MPa, analysis temperature is 30°C, analysis pressure is 5MPa, CO 2 The flow rate is 18L / h, remove oily substances, release raw material residues, add 70% ethanol solution for microwave extraction 3 times, microwave extraction power is 600w, filter to obtain extract, add 3% (W / V) of the liquid volume to the extract Activated carbon was decolorized at 50°C for 40 minutes, filtered to obtain a decolorized solution, and the decolorized solution was added to a hollow cellulose ultrafiltration membrane system with a molecular weight cut-off of 10,000 for ultrafiltration, and the permeate was added to a hollow cellulose nanofiltration membrane system with a molecular weight cut-off of 500 Concentrate and dry the retentate to obtain the crude product; use petroleum ether-ethyl acetate-ethanol-wa...

Embodiment 3

[0025] Blu-ray flower bulbs are crushed, weighed 3kg, and added to a supercritical extraction kettle for supercritical CO 2 extraction, extraction temperature is 50°C, extraction pressure is 20MPa, analysis temperature is 40°C, analysis pressure is 7MPa, CO 2 The flow rate is 20L / h, remove the oily substances, release the raw material residue, add 70% ethanol solution for microwave extraction twice, the microwave extraction power is 800w, filter to obtain the extract, add 3% of the liquid volume (W / V) to the extract Activated carbon was decolorized at 60°C for 30 minutes, filtered to obtain a decolorized solution, and the decolorized solution was added to a hollow cellulose ultrafiltration membrane system with a molecular weight cut-off of 6000 for ultrafiltration, and the permeate was added to a hollow cellulose nanofiltration membrane system with a molecular weight cut-off of 600 Concentrate and dry the retentate to obtain the crude product; use petroleum ether-ethyl acetate...

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PUM

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Abstract

The invention relates to a method for extracting and separating squill saponin C, which comprises the steps of: crushing blue flower bulbs, adding the crushed blue flower bulbs into a supercritical extraction kettle, and carrying out supercritical CO2 extraction; removing oil substances, and discharging raw material residue; adding 50-80% ethanol solution for microwave extraction; adding activated carbon into extract for decoloration, and filtering to obtain decolored liquid; adding the decolored liquid into an ultrafiltration membrane system, and carrying out ultrafiltration; adding permeate liquid into a nanofiltration membrane system, and carrying out concentration; drying retentate to obtain crude product; and finally, separating and purifying the crude product by a high-speed counter-current chromatography method to obtain the squill saponin C. The method is large in preparation quality, less in sample loss, short in production period, high in efficiency and low in pollution, thus being suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of separation of natural products, and in particular relates to an extraction and separation method of squillin C. Background technique [0002] Agapanthus saponin A (Agapanthus saponin A) is a triterpenoid saponin with the molecular formula C 65 h 104 o 33 , molecular weight 1413.51. Scallop saponin C has inhibitory activity on cyclic adenosine monophosphate (cAMP) phosphodiesterase, and its IC 50 It is 112μM; it can inhibit the incorporation of TPA-induced 32P into HeLa cell phospholipids, and the inhibition rate is 22.5% at 5μg / ml. Scallion saponin C is mainly derived from the blue light flower of Liliaceae (Liliaceae) Chionndoxa gigantea Hort.'s bulb. [0003] At present, there are no relevant literature and patent reports on the industrial extraction and purification method of squillion C in China. Contents of the invention [0004] The technical problem to be solved by the present invention is to provid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J21/00
CPCY02P20/54
Inventor 刘东锋
Owner NANJING ZELANG MEDICAL TECH