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Preparation method of anti-oxidant

A technology for antioxidants and manufacturing methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, anti-toxins, etc., can solve the problems of no discovery, low SOD activity, high SOD activity, etc., and achieve easy operation and excellent safety Effect

Inactive Publication Date: 2012-12-19
KAO CORP
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Problems solved by technology

However, as can be seen from the examples, the SOD activity is low when only guava extract (sample 1) and only rice bran and soybean fermented product (sample 2) are used, and the active oxygen scavenging effect is insufficient.
And, because the acquisition of guava leaves is limited by seasons, there are difficulties in practical use of this method as well.
[0006] As mentioned above, in the prior art, among substances having an active oxygen scavenging effect, no substance having both high SOD activity and practical use has been found.

Method used

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Embodiment 1～ Embodiment 5

[0046] As microorganisms for producing an antioxidant (active oxygen scavenger) from okara, microorganisms of the genus Bacillus shown in Table 1 below were used.

[0047] Table 1

[0048]

[0049] * The above-mentioned microorganisms were all obtained from Jiangnan University and preserved in CICIM, the Industrial Microbiology Resource and Information Center of Chinese Universities.

[0050] The above strain obtained by culturing in YPD (Yeast Extract Peptone Dextrose Medium) medium at 30°C was inoculated into an Erlenmeyer flask (capacity 300ml) containing 100ml of antioxidant production medium, and cultured at 30°C for 72 hours. The medium contained 10 w / v% okara and 1 w / v% glucose. Wherein, the preparation of soybean dregs is obtained by soaking soybeans in water for 12 hours to make soybeans fully absorb water, then grinding and crushing the soybeans that have absorbed water, filtering and removing liquid components, and draining remaining residues.

[0051] Then, t...

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Abstract

The invention relates to a preparation method of an anti-oxidant. According to the method, bacillus microbes are cultured in a culture medium containing soybean residue, and the anti-oxidant is obtained from a supernatant. The invention also provides a high-SOD-activity anti-oxidant obtained through the preparation method. With the preparation method provided by the invention, a specific microbe can be utilized; soybean residue which is a by-product of feedstuff processing industry and the like is adopted as a main raw material of the culture medium; and the anti-oxidant is obtained with low cost and high efficiency.

Description

technical field [0001] The present invention relates to a production method of an antioxidant and an antioxidant with high SOD activity obtained by the production method. Background technique [0002] SOD is the English abbreviation of superoxide dismutase (superoxide dismutase), which is an antioxidant enzyme synthesized in the human body. SOD decomposes harmful superoxide radicals produced by various reasons in the human body into hydrogen peroxide (a type of active oxygen), and then further decomposes hydrogen peroxide by the action of catalase and peroxidase It is completely harmless water and oxygen, so it has the effect of reducing the concentration of active oxygen in the body, and has the effect of preventing and improving skin roughness, spots, and wrinkles caused by active oxygen. Therefore, SOD products have been widely used in the fields of food, cosmetics, and pharmaceuticals as a method for preventing aging. However, most of the existing SOD products are deri...

Claims

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Application Information

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IPC IPC(8): C12N9/02A23L1/30A61K8/96A61K8/66A61K38/44A61K36/48A61Q19/00A61P39/06C12R1/10
Inventor 孔凡旗
Owner KAO CORP
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