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Bionic enzymolysis preparation method of leech powder

A technology of bionic enzymolysis and leech powder, which is applied in the direction of hydrolyzed protein components, medical raw materials derived from leeches/worms, blood diseases, etc., to achieve high bioavailability, strong physiological activity, and improve bioavailability

Inactive Publication Date: 2012-12-26
FENGTAI COUNTY WENLIANG REAL ESTATE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But all there are various defects in the various preparation methods of existing leech products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A biomimetic enzymolysis preparation method of leech powder, comprising the following steps:

[0019] (1) Freeze-dry live leeches cultivated or purchased at -22°C until the water content is below 5%, take 500g, and crush them into powder;

[0020] (2) Add 10 times the amount of water to homogenate, adjust the pH to 2.0 with citric acid or dilute hydrochloric acid, add 2% pepsin (enzyme activity: 1:3000) by weight of leech powder, incubate at 40°C for 2.5 hours, and then adjust pH to 8.0, add trypsin, enzyme dosage 5000NFU·g -1 , incubating at 50°C for 8 hours for enzymatic hydrolysis;

[0021] (3) Add an appropriate amount of dextrin and spray dry at a temperature of about 40 degrees to obtain a finished product.

Embodiment 2

[0023] A biomimetic enzymolysis preparation method of leech powder, comprising the following steps:

[0024] (1) Freeze-dry live leeches cultivated or purchased at -22°C until the water content is below 5%, take 500g, and crush them into powder;

[0025] (2) Add 10 times the amount of water to homogenate, adjust the pH to 1.5 with citric acid or dilute hydrochloric acid, add 1.5% pepsin (enzyme activity: 1:3000) by weight of leech powder, incubate at 45°C for 1 hour, and then adjust pH to 8.5, add trypsin, enzyme dosage 5000NFU·g -1 , incubating at 50°C for 7.5 hours for enzymatic hydrolysis;

[0026] (3) Add an appropriate amount of dextrin and spray dry at a temperature of about 40 degrees to obtain a finished product.

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Abstract

The invention relates to a bionic enzymolysis preparation method of leech powder. The bionic enzymolysis preparation method comprises the following steps of: (1) freezing and drying a leech living body until a moisture content is lower than 5% and smashing into powder; (2) adding water to evenly stir to be pasty, adjusting the pH to be 1.0-3.0, adding pepsin at the temperature of 35-45 DEG C for heat preservation enzymolysis for 0.5-4 hours, then adjusting the pH to be 8.0-8.6, and adding trypsin at the temperature of 45-50 DEG C for heat preservation enzymolysis for 7.5-8 hours; and (3) conducting spray drying on enzymolysis products. The bionic enzymolysis preparation method uses a method that the leech living body is freezed and dried and evenly smashed into the powder to be pasty, then a human body digestion process is simulated, and a bionic enzymolysis method of pepsin enzymolysis and trypsin enzymolysis is adopted to conduct enzymolysis on macromolecule proteins in a leech to be micromolecule peptide. The obtained enzymolysis liquid has anti-freezing and fibrinolysis activities, is strong in vigour than decocting liquid, can improve bioavailability to the greatest extent, and reduces the occurrence rate of toxic and side effects.

Description

technical field [0001] The invention relates to a preparation method of leech powder. Background technique [0002] Leech is a precious medicinal material. The general homogenization method, water decoction, water decoction and alcohol precipitation and other processes have a low ointment rate, and the utilization rate of the medicinal material is low, and the extracted medicinal residue is still relatively active; the fine powder of leech can be taken directly , only a small part is digested and absorbed by the gastrointestinal tract, and most of them are excreted in the original form, which greatly wastes medicinal materials. The leech decoction and its alcohol-soluble part contain anticoagulant active substances, and the water decoction and its non-alcoholic part contain fibrinolytic active substances. Has very good medicinal value. But all there are various defects in the various preparation methods of existing leech products. Contents of the invention [0003] The ...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K35/62A61P7/02
Inventor 梁志森梁文亮
Owner FENGTAI COUNTY WENLIANG REAL ESTATE
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