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Process for the preparation of a biomass comprising plantaricin and uses thereof in medical field

A technology of plant lactobacillus and biomass, which is used in the preparation of mixtures to stimulate the barrier function of intestinal cells or human epidermal keratinocytes to achieve the effect of enhancing the barrier function

Inactive Publication Date: 2015-07-15
GIULIANI SPA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, in the current state of the art, there are no publications or patents focusing on the role of signaling molecules involved in bacterial cell communication mechanisms associated with the human epidermis

Method used

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  • Process for the preparation of a biomass comprising plantaricin and uses thereof in medical field
  • Process for the preparation of a biomass comprising plantaricin and uses thereof in medical field
  • Process for the preparation of a biomass comprising plantaricin and uses thereof in medical field

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0062] Example 1: Synthesis and purification of phytolactin-type peptide pheromones and study of their effects on epidermis and Caco-2 cells.

[0063] L. plantarum DC400 (DSM 23213) and L. rossiae DPPMA174 ( DSM), previously isolated from a "natural starter", has been propagated at 30°C for 24 hours in a modified MRS medium (mMRS) containing, in addition to the usual ingredients, 5% maltose and 10% starter water - final pH 5.6.

[0064] Cells were incubated for 24 hours, harvested by centrifugation (10.000 × g for 15 min at 4°C), washed twice with 50 mM phosphate buffer, pH 7.0, and then resuspended in water at a cell density of 9.0 log UFC / ml , inoculated (4%, each) on WFH medium under monoculture or co-cultivation conditions (Gobbetti, 1998.The sourdough microflora:interactions of lactic acid bacteria and sourdoughs (starter flora: lactic acid bacteria and starter interaction). Trends Food Ski Technol ("Food Science and Technology Trends") Vol. 9 pp. 267274). Following ...

example 2

[0103] Example 2: Research on the Effect of Biomass of the Present Invention and Plant Lactobacillus A in Wound Healing

[0104] method

[0105] Cultured human keratinocytes were incubated with phytolactin A (2.5 μg / ml) or biomass containing phytolactin A for 1 hour.

[0106] At the end of the incubation, the cells were washed and the medium was restored.

[0107] wound healing

[0108] The assay involved mechanical disruption of the continuity of the cell monolayer and was intended to assess whether the treatment favored the ability of keratinocytes to migrate beyond the damaged margin and thus "heal the injury".

[0109]To this end, keratinocyte monolayers treated as described above were cultured for an additional 24 hours after stimulation, then fixed and stained with hematoxylin / eosin staining. Images were observed using an optical microscope with a 5X objective.

[0110] For each image, detect and calculate the maximum cell migration limit and the distance or the ga...

example 3

[0127] Example 3: Phytolactin A: a study of its role in maintaining and repairing skin barrier function

[0128] This study is based on the use of plantaractin A obtained from the co-cultivation of L. plantarum DC400 (DSM 23213) and L. rossiae DPPMA174 (DSM 23214), with the aim of obtaining the following objectives:

[0129] The effect of biomass on wound repair was evaluated by studying the migration and proliferation of human keratinocyte monolayers (NCTC2544) in comparison to positive controls (untreated cells) and commercially available / negative controls of known activity. Treated three times (24, 48, 72 hours) with different concentrations of test plant lactobacillus (after determination by cytotoxicity test).

[0130] Evaluate mediator modulation such as IL-8 by real-time PCR in comparison to positive controls (untreated cells) and commercially available / negative controls of known activity at the desired time and concentration , KGF (keratinocyte growth factor), TGF-β...

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Abstract

The present invention concerns methods for administering to a subject plantaricins A, N or K and / or a biomass having at least one plantaricin selected from plantaricins A. K or N or mixtures thereof and Lactobacillus plantarum DSM 23213 and Lactobacillus rossiae DSM 23214 lactic acid bacteria and / or the biomass as an active principle in association with one or more pharmaceutically acceptable excipients and / or adjuvants

Description

technical field [0001] The invention relates to a preparation method of biomass containing plantaricin and its use in the medical field. Particularly, the present invention relates to the preparation method of plant lactobacillus A, N or K or their mixture, or the preparation method that comprises one or more above-mentioned plant lactobacillus together with the biomass of the lactic acid bacteria used for preparation, and their use for stimulating the barrier function of enterocytes or human epidermal keratinocytes. Background technique [0002] It is known that the epidermis and intestinal wall have a barrier function against the external environment and harmful substances therein. The barrier function of the epidermis and intestinal wall can be weakened due to a variety of factors. One of the more common conditions that lead to changes in the barrier function of the epidermis is exposure to various triggering environmental factors. Under normal conditions, however, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61Q19/08C12R1/25A61K35/74
CPCA61K38/164C12R1/225A61K35/74C12N1/20C12R1/25C07K14/335C12P39/00A61P1/04A61P17/00A61P17/02A61P43/00C12R2001/225C12N1/205C12R2001/25
Inventor G·朱利亚尼A·本纳杜斯M·戈贝蒂R·迪家格诺M·德安格利斯M·加拉索
Owner GIULIANI SPA
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