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Preparation method of material with oriented growth structure cells

A technology of oriented growth and cells, applied in the coating and other directions, can solve the problem of unexpanded, and achieve the effect of high pattern resolution, precise control and obvious contrast.

Active Publication Date: 2013-12-25
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Then in 1991, Jean C Wittmann et al. reported that rubbing PFTE induced small molecules to crystallize on it, and this technology has further developed in terms of thin films and interface materials (Nature1991,352,414; Macromolecules 2010,43,7604; Adv .Funct.Mater.2011,21,4047.) But this technique has not been extended to biomaterials where microstructural regulation is increasingly important

Method used

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  • Preparation method of material with oriented growth structure cells
  • Preparation method of material with oriented growth structure cells
  • Preparation method of material with oriented growth structure cells

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A method for preparing a material with oriented growth structure cells, comprising the following steps:

[0040] 1) Put the clean glass substrate (flat or curved surface) and the polytetrafluoroethylene rod on a hot stage heated to 280°C for 10 minutes, and the dynamic friction coefficient of the glass substrate is 0.06~0.7;

[0041] 2) Fix the glass substrate, apply a pressure of 50~500N vertically downward to the substrate on the PTFE rod, and apply a thrust of 5~300N in the horizontal direction, so that the PTFE rod rubs against the glass, and then cool at room temperature , slowly pull the glass substrate in the PCL solution (1w / v%) along the rubbing direction, and after the solvent evaporates naturally, continue heating on a hot stage at 55°C for 2 hours, and the oriented PCL compounded on the PTFE matrix can be obtained Thin film (flat sample No. 1#, curved surface sample No. 2#).

[0042] figure 1 A is the operation schematic diagram of preparing sample 1# in emb...

Embodiment 2

[0047] A preparation method of a material with oriented growth structure cells, the steps are the same as in Example 1, the change is that growing rat bone marrow mesenchymal cells (BMMSCs) are cultured on sample 1#.

[0048] image 3 a, b, c are scanning electron microscope images of rat bone marrow mesenchymal stem cells cultured with sample 1# for 3 days; image 3 d, e, f are fluorescence microscope images of rat bone marrow mesenchymal stem cells cultured with sample 1# after 3 days; it can be seen from the figure that the BMMSCs cells cultured on the material obtained by the preparation method of the present invention have good cell orientation and cell adhesion and proliferative performance remained good.

Embodiment 3

[0050] A preparation method of a material with oriented growth structure cells, the steps are the same as in Example 1, except that Chinese hamster ovary fibroblasts (CHO) were cultured on sample 2# for 7 days.

[0051] Figure 4 a, b are fluorescence microscope images of Chinese hamster ovary fibroblasts cultured with sample 2# for 7 days.

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Abstract

The invention discloses a preparation method of material with oriented growth structure cells, wherein the method comprises the following steps: coating a biocompatible polymer film on a friction-oriented substrate of a polymer, forming a binary composite film of polymer / biocompatible polymer by thermal treatment, and then performing cell culture on the composite film so as to obtain the material with oriented growth structure cells. According to the preparation method, the material with oriented growth structure cells can be prepared on a large scale and with low cost.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a preparation method of a material with oriented growth structure cells. Background technique [0002] The interaction between cells and materials is one of the important fields of biomaterials and tissue engineering research. Cells in most mammals must adhere to materials in order to proliferate, migrate and differentiate. The surface properties of materials, whether it is the difference in surface topological microstructure or the difference in chemical signals, have a great impact on cell behavior (including from initial adhesion to proliferation, and even differentiation and final formation of new tissues). Called contact induction (Contact Guidance). Cells in most normal tissues are not chaotic, but have a certain orderly orientation structure, for example: tendon cells, bone cells, cardiomyocytes, endothelial cells and smooth muscle cells that constitute blood vessels ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/04C08J5/14
Inventor 牛忠伟王艳明蒋士冬
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI