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N-tail-end B-type brain natriuretic peptide precursor chemiluminiscence immunity quantification detecting kit and preparation method thereof

A chemiluminescence immunoassay and quantitative detection technology, applied in the field of immunoassay medicine, can solve the problems of short validity period of reagents, radioactive contamination, and narrow detection range, and achieve the effects of good stability, easy operation and high sensitivity

Active Publication Date: 2013-01-02
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The problem to be solved by the present invention is to provide a chemiluminescent immunoassay kit for N-terminal B-type brain natriuretic peptide (NT-proBNP) and its preparation method, which avoids the short validity period, radioactive contamination, and cumbersome operation of radioimmunoassay reagents. Shortcomings, and solve the defects of low sensitivity, narrow detection range and high cost

Method used

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  • N-tail-end B-type brain natriuretic peptide precursor chemiluminiscence immunity quantification detecting kit and preparation method thereof
  • N-tail-end B-type brain natriuretic peptide precursor chemiluminiscence immunity quantification detecting kit and preparation method thereof
  • N-tail-end B-type brain natriuretic peptide precursor chemiluminiscence immunity quantification detecting kit and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Preparation of N-terminal B-type natriuretic peptide precursor (NT-proBNP) chemiluminescence immunoquantitative detection kit

[0034] N-terminal B-type brain natriuretic peptide precursor (NT-proBNP) chemiluminescent immunoquantitative detection kit, characterized in that the kit includes: NT-proBNP antibody-coated plate, NT-proBNP antibody enzyme conjugate, NT- proBNP calibrator, NT-proBNP quality control substance, 20-fold concentrated washing solution, luminescent solution A and B.

[0035] The N-terminal B-type brain natriuretic peptide precursor chemiluminescent immunoquantitative detection kit was prepared by the following method:

[0036] 1) Preparation of NT-proBNP antibody-coated plates

[0037]Dilute the NT-proBNP antibody to 1-10ug / mL with 0.02M phosphate buffer, add it to a 96-well white microwell plate, and coat at 37°C for 2 hours; discard the liquid in the well and wash with pH7.4 PBS buffer plate, then add 0.5% BSA-containing phosphate buff...

Embodiment 2

[0061] Example 2: Preparation of N-terminal B-type BNP chemiluminescent immunoquantitative detection kit

[0062] The kit of the present invention was prepared in the same manner as in Example 1, except that the solid phase carrier was a 48-well microwell plate.

Embodiment 3

[0063] Embodiment 3: the using method of kit of the present invention

[0064] 1) Equilibrate the test kit at room temperature (18-25°C) for 30 minutes.

[0065] 2) Prepare lotion: Dilute the concentrated lotion with distilled water at 1:20 (1mL lotion plus 19mL distilled water). If the concentrated lotion has crystals, place the concentrated lotion at room temperature or 37°C until the crystals dissolve before diluting.

[0066] 3) Preparation of luminous liquid: take an appropriate amount of luminous liquid A and B liquid and mix equal volumes 5 minutes before use.

[0067] 4) Take out an appropriate amount of coated slats according to the needs of the experiment. Set up 1 well for blank control and 2 wells for each calibrator. Add 50 μL of NT-proBNP calibrator or quality control substance to each well, add 50 μL sample to the sample well, and add no calibrator or sample to the blank control.

[0068] 5) Add 50 μL of NT-proBNP enzyme conjugate to each well, except the bl...

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Abstract

The invention discloses an N-tail-end B-type brain natriuretic peptide precursor chemiluminiscence immunity quantification detecting kit. The kit comprises an NT-proBNP anti-body coated plate, an NT-proBNP antibody enzyme conjugate, an NT-proBNP correcting substance, an NT-proBNP quality control substance, 20-time concentrated washing liquid, luminance liquid A and luminance liquid B. The invention further discloses a preparation method of the N-tail-end B-type brain natriuretic peptide precursor chemiluminiscence immunity quantification detecting kit. Compared with the existing kit, the kit is simple to operate, safe and free of environment pollution. The kit has the advantages of being wide in concentration range of a detected sample, long in validity period of a reagent, good in stability and the like.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine, in particular to an N-terminal B-type brain natriuretic peptide (NT-proBNP) chemiluminescent immunoquantitative detection kit and a preparation method thereof. Background technique [0002] Brain Natriuretic Peptide (BNP), also known as B-type Natriuretic Peptide, is another member of the natriuretic peptide system after atrial natriuretic peptide (ANP). Sudoh was separated from the pig brain in 1988 and got its name. In fact, it mainly comes from the ventricle. Cardiomyocytes first synthesize proBNP of 108 amino acids, also known as BNP precursor (proBNP). Under the action of protease, proBNP is decomposed into N-terminal B-type brain natriuretic peptide precursor (NT-proBNP) and biologically active hormone BNP, two polypeptides are released into the blood circulation. Both originate from the same source and are secreted equimolarly. Some studies in recent years have shown that the N-te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543
Inventor 刘萍宋启超董婷婷李克锦范利花
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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