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Phthalate degrading strain and application thereof

A technology of phthalate and dibutyl phthalate, applied in the field of phthalate degrading bacteria, can solve problems such as embryonic development toxicity, achieve high degradation ability and solve environmental pollution problems. Effect

Active Publication Date: 2013-01-09
ZHEJIANG UNIV OF TECH
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Problems solved by technology

and have certain toxic effects on embryonic development

Method used

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  • Phthalate degrading strain and application thereof
  • Phthalate degrading strain and application thereof
  • Phthalate degrading strain and application thereof

Examples

Experimental program
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Embodiment 1

[0023] 1 Materials and methods

[0024] 1.1 Reagents and instruments

[0025] DMP and DEP were purchased from Shanghai Lingfeng Reagent Co., Ltd., DBP was purchased from Zhejiang Linping Chemical Reagent Factory, DEHP was purchased from Sinopharm Chemical Reagent Co., Ltd., methanol (chromatographic grade, Tianjin Siyou Chemical Co., Ltd.), and the rest of the reagents were analyzed pure. JEOL JEM-1230 transmission electron microscope (Japan), Agilent1100 high performance liquid chromatography (USA). 1.2 Medium

[0026] Basic inorganic salt medium: K 2 HPO 4 ·3H 2 O 1.0g / L, NaCl 1.0g / L, (NH 4 ) 2 SO 4 0.5g / L, MgSO 4 ·7H 2 O 0.4g / L, CaCl 2 0.0755g / L, FeCl 3 ·6H 2 O 0.0143g / L, pH7.0.

[0027] Selection medium: Add appropriate amount of DBP methanol solution to each liter of inorganic salt medium, so that the final concentration of DBP is 125mg / L.

[0028] LB medium: peptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000mL, pH 7.0, sterilized at 121°C for 2...

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Abstract

The invention provides a strain Arthrobacter sp.ZJUTW with high phthalate degrading property and an application of the strain. The strain is conserved in the China Center for Type Culture Collection (address: Wuhan University, Wuhan City, China 430072), the preservation number (CGMCC No) is 2012246 and the conservation date is June 24, 2012. The new strain with high phthalate degrading property is provided, the diethyl phthalate (DEP) and dibutyl phthalate (DBP) degrading conditions of resting cells are optimized and the environmental pollution problem of phthalate compounds can be effectively solved.

Description

(1) Technical field [0001] The invention relates to a phthalate-degrading bacterium—Arthrobacter sp. ZJUTW and its application in microbial degradation of phthalate. (2) Background technology [0002] Phthalic acid esters (PAEs) are artificially synthesized refractory organic compounds widely used in the world, mainly used in plastic plasticizers, coatings, paints and other chemical production. Among the 129 priority pollutants announced by the US Environmental Protection Agency (EPA) in 1999, there are 6 PAEs compounds, and my country has also included di-n-octyl phthalate (DOP) and dibutyl phthalate (DBP), diethyl phthalate (DEP) and dimethyl phthalate (DMP) are listed as environmental priority pollutants. Existing evidence shows that: PAEs are a class of environmental endocrine disruptors, and their most obvious harm is to reduce reproductive function, cause certain damage to the reproductive system of animals and humans, and can cause testicular atrophy, sperm reduction,...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/06A62D101/28
Inventor 邱乐泉陈阳钟卫鸿吴石金李烜桢钟莉王子超
Owner ZHEJIANG UNIV OF TECH
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