Ligusticum chuanxiong hort extract and preparation method and application thereof

A chuanxiong extract and the technology of the extract, which are applied in the field of traditional Chinese medicine chemistry, can solve the problems of lipid-lowering and unclear anti-atherosclerosis activities, and achieve the effects of inhibiting the formation of atherosclerotic plaques, and the preparation method is efficient and convenient

Inactive Publication Date: 2013-01-16
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, most of the current researches focus on Ligustrazine and Ferulic Acid, and it is no

Method used

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  • Ligusticum chuanxiong hort extract and preparation method and application thereof
  • Ligusticum chuanxiong hort extract and preparation method and application thereof
  • Ligusticum chuanxiong hort extract and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The preparation of embodiment 1 Rhizoma Chuanxiong extract

[0030] 1) Weigh 1 kg of Ligusticum Chuanxiong, soak in 95% ethanol for 10 hours, heat and reflux with 4000ml 95% ethanol for 3 times, each time for 2 hours, combine the extracts, and concentrate under reduced pressure on a rotary evaporator until the relative density of water is 1.5. liquid.

[0031] 2) Add the concentrated solution to 500ml water to disperse into a suspension, extract 3 times with 500ml petroleum ether, combine the extracts, recover the solvent to dryness, repeat the extraction once according to the above steps, recover the solvent to dryness, and obtain the extract concentrate.

[0032] 3) Take the extract concentrate obtained in step 2), and separate it through a 200-300 mesh forward silica gel column (coarse-pore silica gel was purchased from Qingdao Ocean Chemical Factory Branch), with a column height of 50 cm and a column diameter of 4.45 cm. Carry out elution with the mixed liquid of s...

Embodiment 2

[0033] The preparation of embodiment 2 Rhizoma Chuanxiong extract

[0034] 1) Weigh 1 kg of Ligusticum Chuanxiong, soak in 95% ethanol for 10 hours, heat and reflux with 4000ml 95% ethanol for 3 times, each time for 2 hours, combine the extracts, and concentrate under reduced pressure on a rotary evaporator until the relative density of water is 1.5. liquid.

[0035] 2) Add the concentrated solution to 500ml water to disperse into a suspension, extract 3 times with 500ml petroleum ether, combine the extracts, recover the solvent to dryness, repeat the extraction once according to the above steps, recover the solvent to dryness, and obtain the extract concentrate.

[0036] 3) Take the extract concentrate obtained in step 2), and separate it through a medium-pressure preparative column filled with a forward silica gel column of 300-400 mesh (silica gel was purchased from Qingdao Ocean Chemical Factory Branch), with a column height of 50 cm and a column diameter of 7 cm. Use pet...

Embodiment 3

[0037] The analysis of embodiment 3 Rhizoma Chuanxiong extract

[0038] Weigh 4.32 mg of the Rhizoma Chuanxiong extract prepared in Example 1, add 0.5 ml of ethyl acetate to dissolve, and transfer an appropriate amount to a brown liquid phase sampling bottle for liquid chromatography-mass spectrometry analysis.

[0039] The analysis conditions are: injection volume 5ul; flow rate 0.8ml / min, column temperature 30°C; mass-to-charge ratio: 100-1000m / z. Liquid chromatography mobile phase composition is 0.01% (volume ratio) formic acid-water solution (A) and acetonitrile solution (B), liquid chromatography gradient is: 0min 30% acetonitrile; 0~45min, acetonitrile concentration rises to 95% from 30% , the rising speed is 1.44% / min; 45-50min, keep the acetonitrile concentration at 95%. The analytical spectrum is attached figure 1 shown.

[0040] Liquid chromatography-mass spectrometry analysis shows that the Rhizoma Chuanxiong extract prepared in Example 1 has an obvious molecular...

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Abstract

The invention relates to a ligusticum chuanxiong hort extract and a preparation method and application thereof. The ligusticum chuanxiong hort extract contains 30 to 90 mass percent of ligustilide, and can be used for preparing anti-atherosclerosis medicines or lipid-lowering medicines.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine chemistry, and in particular relates to an extract of Rhizoma Chuanxiong, its preparation method and application. Background technique [0002] Coronary atherosclerotic heart disease is mainly composed of coronary atherosclerosis, inflammation (rheumatism, syphilis, Kawasaki disease and vaso-occlusive vasculitis, etc.), coronary artery spasm, embolism, connective tissue disease, trauma and congenital Myocardial ischemia and hypoxia are caused by deformities and other reasons, and the main symptoms are angina pectoris and myocardial infarction. Because the vast majority (95-99%) of coronary heart disease is caused by coronary atherosclerosis. The traditional idea is that atherosclerosis (AS) is a disease caused by lipid accumulation, so lipid-lowering therapy has always been the focus of anti-atherosclerosis drug development and atherosclerosis treatment. The mechanism of action of sta...

Claims

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Application Information

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IPC IPC(8): A61K36/236C07D307/88A61P3/06A61P9/10
Inventor 程翼宇王怡刘二伟
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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