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Porous hydrogel microsphere and preparation method thereof

A technology of porous hydrogel and microspheres, applied in the field of biological materials, can solve the problems of impermeability of nutrients, poor biocompatibility of porous microspheres, complicated operation, etc. The effect of good biocompatibility

Pending Publication Date: 2021-12-17
苏州永沁泉智能设备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the currently commonly used hydrogel microspheres have the following problems: (1) The hydrogel lacks pores, which makes the nutrients impermeable and the internal cells cannot survive; (2) The preparation of porous microspheres has poor biocompatibility, cumbersome steps, Unable to achieve one-step preparation of microspheres, which adversely affects the bioactive factors loaded on the microspheres
Literature: Preparation of gelatin-based slow-release microspheres as cell microcarriers, Chemical Journal of Chinese Universities, No. 9, 1776-1780, 2007, adopts the method of secondary swelling of cross-linked gelatin microspheres with water and freeze-drying to prepare porous structure, the method is relatively complicated to operate, and it is difficult to control the growth of ice crystals during the freeze-drying process. It does not participate in the dissolution of cross-linked gelatin and will produce a large number of flocs after freeze-drying, which is not conducive to large-scale preparation.

Method used

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  • Porous hydrogel microsphere and preparation method thereof
  • Porous hydrogel microsphere and preparation method thereof
  • Porous hydrogel microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] like figure 1 Shown:

[0083] 1) Add 0.1g of Span 80 to 100g of liquid paraffin (emulsifier: oily solvent = 0.1:100), stir and dissolve at 60°C to prepare the oil phase;

[0084] 2) Add 0.5g GelMA (5%), 0.025g initiator LAP (0.25%) and 0.1g PEO (1%) to 10mL deionized water, heat and stir in the dark at 50°C to obtain a hydrogel precursor solution;

[0085] 3) Add 1 mL of the precursor solution in the above step 2) to 20 mL of the oil phase prepared in step 1) and heat and stir at a speed of 500 r / min for 20 min to form a water-in-oil emulsion;

[0086] 4) The light intensity is 100mW / cm 2 A 405nm light source irradiates the above-mentioned water-in-oil emulsion for 30 minutes to cause a cross-linking reaction of the microspheres;

[0087] 5) After the reaction is over, stop heating and stirring, make the temperature of the system drop to room temperature, and pour off the upper oil phase;

[0088] 6) Add ether twice the volume of the remaining product (lower layer a...

Embodiment 2～4

[0095] The porous hydrogel microspheres of Examples 2-4 were prepared according to the corresponding added materials and amounts in Table 1 below, with the same operation steps as Example 1.

[0096] Table 1 Added materials and amounts added in Examples 2 to 4

[0097]

Embodiment 5

[0099] like figure 1 Shown:

[0100] 1) Add 0.1 g of Span 80 to 100 g of liquid paraffin (emulsifier: oily solvent = 0.1:100), stir and dissolve at 60°C to prepare an oil phase.

[0101] 2) Add 0.5g GelMA (5%), 0.2g initiator sodium persulfate (SPS) (2%) and 0.1g PEO (1%) to 10mL deionized water, heat and stir in the dark at 50°C to obtain a hydration Glue precursor solution;

[0102] 3) Add 1 mL of the precursor solution in the above step 2) to 20 mL of the oil phase prepared in step 1) and heat and stir at a speed of 500 r / min for 20 min to form a water-in-oil emulsion;

[0103] 4) heating and stirring the above-mentioned water-in-oil emulsion at 60° C. for 1 hour to cause a cross-linking reaction of the microspheres;

[0104] 5) After the reaction is over, stop heating and stirring, make the temperature of the system drop to room temperature, and pour off the upper oil phase;

[0105] 6) Add ether twice the volume of the remaining product (lower layer after removing the...

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Abstract

The invention provides a porous hydrogel microsphere and a preparation method thereof. The preparation method comprises the following steps: (1) dissolving an emulsifier in an oily solvent to prepare an oil phase; (2) dissolving a free radical crosslinking water-soluble polymer, a sacrificial component polymer and an initiator in water to prepare a precursor solution; and (3) adding the precursor solution into the oil phase prepared in the step (1), stirring to form a water-in-oil emulsion, then carrying out a cross-linking reaction, and after the reaction is finished, carrying out post-treatment to obtain the porous hydrogel microsphere. According to the preparation method, the water-in-oil emulsion is formed through emulsion polymerization, the porous hydrogel microsphere is obtained through pore forming in the microsphere forming process, secondary pore forming treatment is not needed, the preparation technology is simplified, and the process is simple and easy to control; and the porous hydrogel microsphere has good biocompatibility, can promote rapid proliferation and extension of cells, can be used as a good tissue engineering scaffold, and has huge application potential in the field of tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a porous hydrogel microsphere and a preparation method thereof. Background technique [0002] Hydrogel is a polymer material with a three-dimensional pore structure. Its good hydrophilicity, high viscoelasticity, and good biocompatibility make hydrogels useful as cell carriers and drug carriers in the fields of tissue engineering and biomedicine. Wide application prospects. [0003] At present, hydrogels used as cell carrier scaffolds usually have large volumes, and small bulk gels are not easy to handle. Therefore, operable miniaturized scaffolds are one of the good solutions. Injectable hydrogel microspheres are a very feasible strategy, using a specific method to make the hydrogel into a spherical shape, and the size meets the injectable requirements. Compared with traditional 3D scaffolds, injectable microspheres avoid surgical transplantation, It provides ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26C08J3/075C08J3/28C08L89/00C08L5/08
CPCC08J9/26C08J3/075C08J3/28C08J2389/00C08J2305/08C08J2471/02C08J2429/04C08J2201/0462
Inventor 高庆简宇航李美琪
Owner 苏州永沁泉智能设备有限公司
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