Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for efficiently extracting liquaemin by utilizing porcine small intestines

A technology of heparin sodium and porcine small intestine is applied in the technical field of efficiently extracting heparin sodium from pig small intestine, which can solve the problems of different salinity requirements, high adsorption rate, small loss of heparin sodium activity, etc. The effect of small adsorption rate and activity loss

Active Publication Date: 2015-01-07
山东绅联药业股份有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to make up for the deficiencies of the existing production technology, the present invention solves the contradiction of different salinity requirements in the process of enzymatic hydrolysis and adsorption, and provides a process for efficiently extracting heparin sodium from the small intestine of pigs. The sodium ion binding rate is high, the adsorption rate is high, and the rapid cooling of the decomposition liquid during adsorption is realized to prevent the rapid cracking of heparin sodium by heparinase, the yield is high, the activity loss of heparin sodium is small, and the quality is stable

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In this embodiment, a process for efficiently extracting heparin sodium from the pig small intestine adopts the following steps:

[0023] (1) Enzymatic hydrolysis:

[0024] Take 420 kg of mucous membranes from 520 pig small intestines and place them in the mucous membrane pool. Through the mucous membrane pump, put the mucous membranes from the mucous membrane pool into the enzymatic hydrolysis tank, add 3360 kg of water into the enzymatic hydrolysis tank, and turn on the mixer for stirring , the rotation speed is 50r / min, stir evenly, then add 840g alkaline protease to the enzymolysis tank, adjust the pH value to 8.0; open the steam valve to make the temperature reach 55°C, add 100kg special salt for casings, and measure the salt with a salinity meter When the temperature reaches 2.5%, keep it warm for 1.5h; after the constant temperature is over, add 100kg of casing special salt, use a salinity meter to measure its salinity to 4.8%, keep warm for 0.5h, then open the s...

Embodiment 2

[0039] In this embodiment, a process for efficiently extracting heparin sodium from the pig small intestine adopts the following steps:

[0040] (1) Enzymatic hydrolysis:

[0041] Take out 310kg of mucous membranes from 477 pig small intestines and place them in the mucous membrane pool, turn on the mucous membrane pump, put the mucous membranes from the mucous membrane pool into the enzymatic hydrolysis tank, add 2790kg of water into the enzymatic hydrolysis tank, turn on the mixer for stirring , the rotation speed is 50r / min, stir evenly, then add 620g of alkaline protease into the enzymolysis tank, adjust the pH value to 8.5; open the steam valve to make the temperature reach 58°C, add 70kg of special salt for casings, and measure the salt with a salinity meter When the temperature reaches 2.2%, keep warm for 1 hour; after the constant temperature is over, add 93kg of special salt for casings, use a salinity meter to measure its salinity to 5.0%, keep warm for 0.2h, then op...

Embodiment 3

[0056] In this embodiment, a process for efficiently extracting heparin sodium from the pig small intestine adopts the following steps:

[0057] (1) Enzymatic hydrolysis:

[0058] Take 520 kg of mucous membranes from 867 pig small intestines and place them in the mucous membrane pool, turn on the mucous membrane pump, put the mucous membranes from the mucous membrane pool into the enzymatic hydrolysis tank, add 3640 kg of water into the enzymatic hydrolysis tank, and turn on the mixer for stirring , the rotation speed is 50r / min, stir evenly, then add 1040g alkaline protease into the enzymolysis tank, adjust the pH value to 9; open the steam valve to make the temperature reach 56°C, add 105kg special salt for casings, and measure the salt with a salinity meter When the temperature reaches 2.4%, keep warm for 1.5 hours; after the constant temperature is over, add 91kg of special salt for casings, measure the salinity as 4.5%, keep warm for 1 hour, then open the steam valve to c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
quality scoreaaaaaaaaaa
quality scoreaaaaaaaaaa
quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention relates to a process for separating biochemical drugs from mucous membranes of porcine small intestines, in particular to a process for efficiently extracting liquaemin by utilizing the porcine small intestines. The process for efficiently extracting the liquaemin by utilizing the porcine small intestines is characterized by comprising the following steps of (1) enzymolysis; (2) adsorption; and (3) elution, sedimentation desalination and dehydration: performing the elution, the sedimentation desalination and the dehydration on filtered resin to prepare the liquaemin. The process for efficiently extracting the liquaemin by utilizing the porcine small intestines has the beneficial effects that the contradiction that the requirements on salinity are different during an enzymolysis process and an adsorption process is skillfully resolved; the liquaemin is prevented from being degraded at high temperature; the loss of the activity of the liquaemin is low; the quality is stable; the production cost is greatly reduced; and the profit can be increased by at least 40 percent.

Description

(1) Technical field [0001] The invention relates to a process for separating biochemical drugs from pig small intestine, in particular to a process for efficiently extracting heparin sodium from pig small intestine. (2) Background technology [0002] Heparin is a mucopolysaccharide that widely exists in mammalian tissues, mainly exists in mast cells, has anticoagulant effect, and is widely used in the treatment of surgery, cerebral thrombosis, myocardial infarction, etc. The molecular weight of heparin is about 3000-37500. Heparin sodium is the sodium salt of heparin. As a natural anticoagulant substance, heparin sodium has been valued by countries all over the world. It is also one of the main export drugs in my country. With the deepening of research, people found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid regulation functions, but also has anti-inflammatory, anti-allergic, anti-viral, anti-cancer and other functions. Pig small intes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/10
Inventor 谢成君李鑫
Owner 山东绅联药业股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com