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Method for promoting tissue culture seedling of anisetree bark to take root rapidly

A technology of tissue cultured seedlings and maple bark, which is applied in the field of plant asexual reproduction, can solve the problems of low survival rate of tissue cultured seedlings transplanted, hindering the application of rapid propagation technology of tissue cultured seedlings, and difficulty in rooting tissue cultured seedlings, etc. The effect of planting survival rate, protecting the diversity of ecological environment and shortening the rooting time

Inactive Publication Date: 2013-08-07
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in the rooting stage of the rapid propagation of the maple bark tissue culture seedlings, the tissue culture seedlings are difficult to take root, or the root system accompanied by a large amount of callus in the root makes the transplanting survival rate of the tissue culture seedlings low, which seriously hinders the growth of the tissue culture seedlings. Application of Rapid Propagation Technology in the Breeding of Maple Bark Seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for promoting rapid rooting of maple bark tissue culture seedlings, comprising the following steps:

[0018] (1) On the ultra-clean workbench, multiply and cultivate the maple bark to obtain aseptic buds with a height of 2 to 3 cm, cut them into single buds, and soak the base in a sterilized rooting agent for 60 min; the rooting agent is : NAA 100 mg / L+IAA 500 mg / L+CPPU 0.001 mg / L +Vc 5.0 mg / L, pH 5.5;

[0019] (2) Take out the single bud of step (1) and put it in a sterile tray for 30 minutes, inoculate it in solid medium 1 / 2MS+ Activated carbon 1g / L + sucrose 20 g / L + agar 5 g / L, pH value 7.0, after 10 days of culture, the stem segment 1 cm above the base slightly swelled, and adventitious roots began to grow from the swollen stem segment and base at 25 days, and at 45 days The growth of adventitious roots is relatively stable, and the number of roots reaches the maximum. The number of roots per plant is about 18.2, and the rooting rate is 100%. The roots ar...

Embodiment 2

[0022] A method for promoting rapid rooting of maple bark tissue culture seedlings, comprising the following steps:

[0023] (1) On the ultra-clean workbench, multiply and cultivate the maple bark to obtain aseptic buds with a height of 2 to 3 cm, cut them into single buds, and soak the base in a sterilized rooting agent for 30 min; the rooting agent is : NAA 800 mg / L+IAA 2000 mg / L+CPPU 0.01mg / L +Vc 2.0mg / L, pH value is 5.5;

[0024] (2) Take out the single bud of step (1) and put it in a sterile tray for 15 minutes, inoculate it in solid medium 1 / 2MS+activated carbon 1g / L+sucrose 20g / L+agar 5g / L, pH value is 7.0, and culture for 5 After 1 day, the stem section 1 cm above the base was slightly enlarged, and adventitious roots began to grow from the enlarged stem section and base at 18 days, and the growth of adventitious roots was relatively stable at 50 days, and the number of roots reached the maximum, and the number of roots per plant was about 23.5 The rooting rate is 100...

Embodiment 3

[0027] A method for promoting rapid rooting of maple bark tissue culture seedlings, comprising the following steps:

[0028] (1) On the ultra-clean workbench, multiply and cultivate the maple bark to obtain aseptic buds with a height of 2 to 3 cm, cut them into single buds, and soak the base in a sterilized rooting agent for 45 min; the rooting agent is : NAA500 mg / L+IAA1500 mg / L+CPPU0.004 mg / L +Vc8.0 mg / L, pH value is 5.5;

[0029] (2) Take out the single bud from step (1) and put it in a sterile tray for 20 minutes, inoculate it in solid medium 1 / 2MS + activated carbon 1g / L + sucrose 20g / L + agar 5g / L, pH value 7.0, culture for 5d Afterwards, the stem segment 1 cm upward from the base was slightly enlarged, and adventitious roots began to grow from the enlarged stem segment and base at 20 days, and the growth of adventitious roots was relatively stable at 45 days, and the number of roots reached the maximum. The number of roots per plant was about 31.3, and the rooting rate ...

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Abstract

The invention discloses a method for promoting tissue culture seedlings of anisetree barks to take roots rapidly. The method comprises the following steps of: carrying out enrichment culture on the anisetree barks to obtain sterile sprouts of 2-3cm in height, cutting into single sprout, and soaking the base parts in a sterilized rooting agent for 20-90min; taking out the single sprout and airing in a sterile disc for 15-30min, and further inoculating into a solid state culture medium, wherein adventitious roots grow from the base parts of the seedlings within 30-50d; and moving the tissue culture seedlings which grow more than 3-7cm and have the roots out of a culture room, hardening the seedling in the culture room for 3d, heeling in sterilized sand in a greenhouse of which the temperature is 22-28 DEG C and the humidity is 75-80% for 30-60d, and subsequently transplanting into humus soil, wherein the rate of survival can be 83.5-94.6%. The method is simple to operate and low in production cost, anisetree bark seedlings can be produced industrially, and basis for sustainable utilization of anisetree barks and ecological environment diversity preservation is provided.

Description

technical field [0001] The invention relates to the technical field of plant vegetative propagation, in particular to a method for promoting rapid rooting of tissue cultured seedlings of maple bark. Background technique [0002] Illicium difengpi (Illicium difengpi K.I.B.et K.I.M.), also known as Fenglang and Gaoshanlong, is a special Chinese medicinal material on the karst rocky mountains in Guangxi. It has the functions of expelling wind and dampness, promoting qi and relieving pain. The treatment of symptoms such as strain and bruises has good curative effect and high medicinal value, and it is also the main raw material of various Chinese patent medicine products. [0003] The maple bark is distributed in the limestone areas of Du'an, Mashan, Dahua, Longzhou, Daxin and other limestone areas in Guangxi Zhuang Autonomous Region or under the sparse forests of stone mountains. The distribution area is narrow, and its stem bark is used for medicinal purposes. Indiscriminate ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 石云平赵志国唐辉付传明黄宁珍唐凤鸾
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI