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Application of Gypensapogenin B in medicament for treating yellow fever virus infection

A yellow fever virus and drug technology, applied in the field of biomedicine, to achieve the effect of huge social benefits and research value

Active Publication Date: 2013-01-23
如东文园投资开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no cases reported in China, but this does not make us relax our vigilance against the virus, because China's climate and geographical environment are complex, and there are many types of mosquitoes, which have the conditions for transmission. At the same time, with the increasing frequency of international exchanges, YFV It is very likely that it will be introduced into China

Method used

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  • Application of Gypensapogenin B in medicament for treating yellow fever virus infection
  • Application of Gypensapogenin B in medicament for treating yellow fever virus infection
  • Application of Gypensapogenin B in medicament for treating yellow fever virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the preparation of the compound Gypensapogenin B tablet involved in the present invention:

[0037] Take 20 grams of compound Gypensapogenin B, add 180 grams of conventional excipients for tablet preparation, mix well, and make 1000 tablets with a conventional tablet press machine.

Embodiment 2

[0038] Embodiment 2: the preparation of the compound Gypensapogenin B capsules involved in the present invention:

[0039] Take 20 grams of compound Gypensapogenin B, add conventional auxiliary materials such as starch 180 grams for the preparation of capsules, mix well, and pack into capsules to make 1000 tablets.

[0040] The following pharmacodynamic experiments will further illustrate its drug activity.

experiment example 1

[0042] A kind of application of Gypensapogenin B in the preparation treatment or prevention yellow fever virus infection medicine, its steps are:

[0043] A. Toxicity test of Gypensapogenin B on Vero cells

[0044] Vero cells (African green monkey kidney cells) are susceptible cells to YFV.

[0045] The experimental steps are as follows:

[0046] 1: Inoculate Vero cells: Use DMEM medium containing 10% (v / v) fetal bovine serum to make a single cell suspension, inoculate 1000-10000 cells per well into a 96-well cell culture plate, and inoculate a volume of 100ul per well ;

[0047] 2: Culture Vero cells: at 37°C, 5% (v / v) CO 2 Under culture conditions, cultivate for 2 days;

[0048] 3: Add Gypensapogenin B: Discard the DMEM medium in each well, add 100ul to each well and dilute to the corresponding concentration (0uM, 0.4uM, 1.2 uM, 3.7uM, 11uM, 33uM, 100uM, 300uM) Gypensapogenin B, add 100ul of DMEM medium without 10% (v / v) fetal bovine serum to the control well;

[0049]...

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PUM

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Abstract

The invention discloses an application of Gypensapogenin B in the preparation of a medicament for treating or preventing yellow fever virus infection. Gypensapogenin B is capable of efficiently inhibiting the propagation of yellow fever virus, but has little toxicity for cells; and Gypensapogenin B is further capable of developing the medicament for treating diseases caused by these viruses, and has wide application prospect. The application of Gypensapogenin B in the preparation of the medicament for treating yellow fever virus infection is firstly disclosed; since the skeleton type belongs to an entirely new skeleton type and the inhibition for yellow fever virus activity is previously unimagined high without any possibility of giving out revelation by other compounds, the application has outstanding substantial characteristics; and simultaneously, the application for preventing and treating yellow fever virus infection is obviously advanced.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to the application of Gypensapogenin B in the preparation of medicines for treating or preventing yellow fever virus infection. Background technique [0002] Yellow fever virus (YFV) is a member of the Flavivirus genus in the family Flaviviridae. Yellow fever virus causes yellow fever, an acute infectious disease. Yellow fever has been designated as a quarantine infectious disease internationally, and China has also designated it as a Class A infectious disease. In 1648, an epidemic of yellow fever was first confirmed in the weekly YUCATAN peninsula. From the 17th to the 19th century, the disease was brought to Europe and North America through transportation. In almost two centuries, yellow fever became one of the most serious plagues in some parts of the United States, Africa, and Europe, causing a large number of deaths. Since the 20th century, the disease has not occ...

Claims

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Application Information

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IPC IPC(8): A61K31/58A61P31/12
CPCY02A50/30
Inventor 施桦王慧吴俊华
Owner 如东文园投资开发有限公司
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