Extraction technology of hyaluronic acid

An extraction process, hyaluronic acid technology, applied in the direction of cosmetic preparations, toiletry preparations, pharmaceutical formulations, etc., can solve the problems of raw material limitations, unable to meet the demand for hyaluronic acid, etc.

Inactive Publication Date: 2013-01-30
肖汉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition to the preparation of hyaluronic acid by fermentation, hyaluronic acid is mostly extracted from cockscombs, human umbilical cords, animal eyeballs and other tissues in China, but due to the limitation of raw materials, it cannot meet the market demand for hyaluronic acid
No domestic manufacturer has extracted hyaluronic acid from eggshells

Method used

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  • Extraction technology of hyaluronic acid
  • Extraction technology of hyaluronic acid
  • Extraction technology of hyaluronic acid

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Experimental program
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Embodiment Construction

[0014] The process steps of the present invention are as follows: sieve the eggshell membrane, soak in distilled water for 5-6 hours, add 1-3% NaCl, stir at 90-95°C for 10-15 minutes, cool to room temperature, add 4000-9000U of protease , enzymatic hydrolysis at 30-80°C for 3-8 hours; centrifuge and filter to collect the supernatant; adjust the pH of the system to 5.0-10.0, add 2 times of 95% ethanol to ethanol for 12 hours; centrifuge to collect the precipitate; dehydrate and dry , Finished product.

[0015] figure 1 It shows that the greater the enzyme dosage, the greater the hyaluronic acid extraction. This is because the more enzyme molecules there are, the higher the probability of contact with the peptide bond of the protein molecule, the faster the enzymatic hydrolysis reaction, and the better the enzymatic hydrolysis effect.

[0016] figure 2 It shows that when the temperature reaches 50°C, the extraction amount of hyaluronic acid is the highest, and the enzymatic ...

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Abstract

The invention relates to an extraction technology of a cosmetic raw material, particularly an extraction technology of hyaluronic acid. The extraction technology disclosed herein has the advantages of easy obtainment of raw materials and high extraction efficiency. The extraction technology disclosed herein comprises the following steps: sieving an eggshell membrane, immersing with distilled water for 5-6h, adding 1-3% of NaCl, stirring for 10-15min at 90-95 DEG C, cooling to room temperature, adding 4000-9000U of protease, conducting enzymolysis for 3-8 h at 30-80 DEG C; centrifuging, filtering, and collecting a supernatant; adjusting the pH value of the system to 5.0-10.0, adding two times of 95% ethanol and conducting alcohol precipitation for 12h; centrifuging, and collecting precipitates; dehydrating, and drying to obtain a finished product.

Description

Technical field: [0001] The invention relates to an extraction process of cosmetic raw materials, more specifically, to an extraction process of hyaluronic acid. Background technique: [0002] Hyaluronic acid (HA) is composed of disaccharide units composed of glucuronic acid and acetylglucosamine repeatedly linked by glycosidic bonds, and is one of several glycosaminoglycans that make up the extracellular matrix. The random curly state of hyaluronic acid in aqueous solution and its hydrodynamic characteristics endow it with important physical properties, namely high viscoelasticity, plasticity, permeability and good biocompatibility, so hyaluronic acid as a functional It is an active cosmetic raw material and has broad application prospects in the fields of cosmetics, health food, cosmetic surgery and medicine. [0003] Significant amounts of hyaluronic acid have been detected in eggshell membranes, and the concentration of hyaluronic acid in the shell membrane has been pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K8/73
Inventor 肖汉
Owner 肖汉
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