Method for reducing viscosity of hyaluronic acid

A technology of hyaluronic acid and viscosity, which is applied in the field of hyaluronic acid to achieve the effects of reducing viscosity, fast dissolution and convenient use

Active Publication Date: 2013-01-30
SHANDONG TOPSCI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problem of inconvenient use due to the high viscosity of hyaluronic acid, the present invention provides a method for reducing the viscosity of hyaluronic acid with simple operation and obvious effect. method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 3g of cosmetic grade HA solid powder (molecular weight 1.24 MDa) into a conical flask, add 40ml of formamide, measure 30ml of triethylamine, slowly pour it into the solution under stirring, and fully stir and mix. Weigh 6g of chloroacetic acid solid into a small beaker, add it into 40ml of formamide solution, stir to dissolve. Slowly pour the chloroacetic acid solution into the HA solution in the Erlenmeyer flask under stirring, and stir and mix well.

[0022] The resulting solution was placed in a 50 °C water bath and stirred for 2 h to obtain a viscous transparent liquid. 300ml of ethanol was added for precipitation. The precipitate was collected, dissolved in purified water, precipitated in ethanol, and this step was repeated 3 times. Dissolve the precipitate with 150 ml of purified water, adjust the pH of the solution to 3.0, add 600 ml of ethanol for precipitation, and wash and dehydrate the obtained precipitate twice. Dry in a vacuum oven at 40°C for 8 ho...

Embodiment 2

[0025] Weigh 5 g of cosmetic grade HA solid powder (molecular weight 1.42 MDa) into a conical flask, add 50 ml of formamide, measure 40 ml of triethylamine, slowly pour it into the solution under stirring, and fully stir and mix. Weigh 10g of chloroacetic acid solid into a small beaker, add it into 50ml of formamide solution, stir to dissolve. Slowly pour the chloroacetic acid solution into the HA solution in the Erlenmeyer flask under stirring, and stir and mix well.

[0026] The resulting solution was placed in a 50 °C water bath and stirred for 4 h to obtain a viscous and transparent liquid. 600ml of ethanol was added for precipitation. The precipitate was collected, dissolved in purified water, precipitated in ethanol, and this step was repeated 3 times. Dissolve the precipitate with 300ml of purified water, adjust the pH of the solution to 3.0, add 1000ml of ethanol for precipitation, and wash and dehydrate the obtained precipitate twice. Dry in a vacuum oven at 40°C f...

Embodiment 3

[0029] Weigh 7g of cosmetic grade HA solid powder (molecular weight 1.42 MDa) into a conical flask, add 70ml of formamide, measure 60ml of triethylamine, slowly pour it into the solution under stirring, and fully stir and mix. Weigh 14g of chloroacetic acid solid into a small beaker, add it into 70ml of formamide solution, stir to dissolve. Slowly pour the chloroacetic acid solution into the HA solution in the Erlenmeyer flask under stirring, and stir and mix well.

[0030] The resulting solution was placed in a 50 °C water bath and stirred for 5 h to obtain a viscous transparent liquid. 1000ml of ethanol was added for precipitation. The precipitate was collected, dissolved in purified water, precipitated in ethanol, and this step was repeated 3 times. Dissolve the precipitate with 400ml of purified water, adjust the pH of the solution to 3.0, add 1200ml of ethanol for precipitation, and wash and dehydrate the obtained precipitate twice. Dry in a vacuum oven at 40°C for 8 h...

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PUM

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Abstract

The invention relates to the technical field of hyaluronic acid, and in particular relates to a method for reducing viscosity of hyaluronic acid. The method comprises the following steps of: adding hyaluronic acid into an alkaline organic solvent; adding chloroacetic acid, reacting at the temperature of 30 to 70 DEG C; and performing post-treatment on the reaction solution to obtain low-viscosity hyaluronic acid. According to the method, HA is modified, and carboxymethyl groups are added into the structure, so that HA is modified on the basis that the original property is kept, the viscosity is reduced, and the dissolubility of HA in the aqueous solution is improved, so that the dissolution of HA is fast, HA is convenient to use, hyaluronic acid is conveniently used in cosmetics or foods, the application range of HA is widened, and the using amount of HA is improved.

Description

technical field [0001] The invention relates to the technical field of hyaluronic acid, in particular to a method for reducing the viscosity of hyaluronic acid. Background technique [0002] Hyaluronic acid (HA) is an acidic mucopolysaccharide composed of (1-β-4) D-glucuronic acid and (1-β-3) N-acetyl-D-glucosamine disaccharide repeat The straight-chain polysaccharides composed of units are widely distributed in the interstitium of various tissues and cells of vertebrates, and are relatively high in cockscombs, vitreous humors, umbilical cords, skin and synovial fluid. It is also distributed in the capsule of Streptococcus. HA has high viscoelasticity, unique moisture retention, excellent biocompatibility and degradability, and is widely used in cosmetics, plastic surgery, medicine and food industries. [0003] HA is a polysaccharide with relatively large molecular weight and has a strong viscosity. When the amount of HA added to cosmetics or food exceeds the specified ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 孙廷华李全义李娟王朋田李刚
Owner SHANDONG TOPSCI BIO TECH
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