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Medium capable of improving polymalic acid yield, use of the medium and method for producing polymalic acid by the medium

A technology of polymalic acid and culture medium, applied in the field of fermentation, can solve problems such as affecting the yield of polymalic acid, and achieve the effects of easy extraction, increased yield and simple process steps

Inactive Publication Date: 2013-01-30
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the endogenous ATP production of the cell reaches saturation, it cannot continue to increase, thus affecting the yield of polymalic acid to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for producing polymalic acid, the concrete steps are:

[0027] a. seed activation

[0028] Aureobasidium pullulans FMT1801 CCTCC NO: M2012223 was inoculated in the seed medium, the inoculation amount was 1:200 according to the volume ratio of Aureobasidium pullulans FMT1801 seed solution to the seed medium, and the formula of the seed medium was glucose 40 g / L, Ammonium nitrate 0.005 g / L, KH 2 PO 4 0.005g / L, ZnSO 4 0.005g / L, MgSO 4 0.005g / L, corn steep liquor 0.5g / L and CaCO 3 10g / L, shake culture at a temperature of 23°C and a rotational speed of 250 rpm for 72 hours to obtain an activated seed solution.

[0029] b. Fermentation

[0030] Inoculate the activated seed liquid obtained in step a into a 5L fermenter containing 3L fermentation medium, the inoculation amount is 1:15 according to the volume ratio of the activated seed liquid and the fermentation medium, and the fermentation medium formula is glucose 50 g / L, chlorine Ammonium chloride 1 g...

Embodiment 2

[0035] The method for producing polymalic acid, the concrete steps are:

[0036] a. seed activation

[0037] Inoculate Aureobasidium pullulans FMT1801 CCTCC NO: M 2012223 into the seed medium, the inoculation amount is 1:800 according to the volume ratio of Aureobasidium pullulans FMT1801 seed liquid to seed medium, and the formula of the seed medium is glucose 100 g / L , ammonium nitrate 5 g / L, KH 2 PO 4 0.3g / L, ZnSO 4 0.3g / L, MgSO 4 0.3g / L, corn steep liquor 3 g / L, citric acid 15 g / L and CaCO 3 50g / L was shaken for 36 hours at a temperature of 28°C and a rotational speed of 300 rpm to obtain an activated seed solution.

[0038] b. Fermentation

[0039] The activated seed liquid obtained in step a is inoculated in the shake flask containing 50mL fermentation medium, the inoculation amount is 1:10 according to the volume ratio of the activated seed liquid and the fermentation medium, and the fermentation medium formula is glucose 200 g / L, ammonium nitrate 5 g / L, KH 2...

Embodiment 3

[0044] The method for producing polymalic acid, the concrete steps are:

[0045] a. seed activation

[0046] Inoculate Aureobasidium pullulans FMT1801 CCTCC NO: M2012223 into the seed medium, the inoculation amount is 1:500 according to the volume ratio of Aureobasidium pullulans FMT1801 seed solution to the seed medium, and the formula of the seed medium is glucose 80g / L, nitric acid Ammonium 4 g / L, KH 2 PO 4 0.2g / L, ZnSO 4 0.2g / L, MgSO 4 0.2g / L, corn steep liquor 2g / L and CaCO 3 40 g / L was shaken for 96 hours at a temperature of 25°C and a rotational speed of 180 rpm to obtain an activated seed solution.

[0047] b. Fermentation

[0048] The activated seed liquid obtained in step a is inoculated in the shake flask containing 50mL fermentation medium, the inoculum is 1:10 according to the volume ratio of the activated seed liquid and the fermentation medium, and the fermentation medium formula is glucose 150g / L, sodium nitrate 4 g / L, KH 2 PO 4 0.2g / L, ZnSO 4 ...

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PUM

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Abstract

The invention discloses a medium capable of improving a polymalic acid yield. The medium contains 1 to 20g / L of citric acid or citrate. The medium can provide external power donors to aureobasidium pullulans in fermentation, and can improve a cellular ATP supply level thereby improving a polymalic acid yield maximally by 57.1%. The medium can be used for producing polymalic acid by aureobasidium pullulan fermentation and has good economic benefits.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a medium for increasing the yield of polymalic acid, and also relates to the application and method of the medium. Background technique [0002] Polymalic acid (PMLA) is polymerized in vivo with L-malic acid as the only monomer. It is a new type of fully biodegradable polymer material with high biocompatibility, biodegradability and biodegradability. Absorbency and other advantages. Because PMLA has a pendant carboxyl group on the side chain, it is easy to react with other functional groups to prepare PMLA derivatives. Compared with materials such as polylactic acid, PMLA shows better advantages in terms of easy metabolism and modification in vivo, and is used in medicine. It has broad application prospects in preparation, food packaging, agriculture and other aspects. Polymalic acid can also be hydrolyzed into a monomer, that is, L-malic acid, also known as 2-hydroxysuccinic acid, ...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12R1/645
Inventor 邹祥戢运超吴小燕昝占全马海霞
Owner SOUTHWEST UNIVERSITY
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