Extraction method of wheat peptone

Abstract

The invention provides an extraction method of botanical microorganism culture medium material, namely, an extraction method of wheat peptone. The public agricultural product of wheat is taken as the main biological raw material. The extraction method includes crushing the wheat, mixing the wheat with water for dissolution, adjusting the pH during heating, adding a compound protease, performing standing and hydrolysis at the constant temperature, siphoning supernatant, and performing mechanical pressure maintaining, filter, precipitation, precision filter, ultra-filtration, vacuum concentration, spray drying and sample spot check and packaging to obtain the finished product. According to the extraction method, the compound protease is used for hydrolysis under relative low temperature condition, so that hydrolysis of wheat protein is more thorough, and the defects of pasty wheat starch due to denaturation and damage to nutrition under a high temperature curing condition in the existing process are overcome simultaneously; additionally, problems of high content of starch fibers, high separating difficulty and the like in the wheat are solved; precipitation and precision filter processes are adopted and the purity of the wheat peptone is effectively guaranteed; and separation, concentration and purification of small and large molecules are achieved through further ultra-filtration.

Description

technical field [0001] The invention relates to a method for extracting plant-derived microbial culture medium materials, that is, a method for extracting wheat peptone using wheat, a popular agricultural product, as the main biological raw material. Background technique [0002] The existing related protein extraction technology is mainly aimed at some animal tissues and soybean protein. The protein structure of animal tissue is generally relatively loose. At the same time, in order to facilitate hydrolysis, the existing technology generally uses the raw material tissue to go through the process of cooking (above 120°C) to denature the protein structure, and then use trypsin, pepsin, papain, alkaline Conventional enzymes such as protease or medium-acid protease perform single hydrolysis; meanwhile, the separation means used in the prior art is relatively simple, using a cartridge filter or a small plate-and-frame filter for solid-liquid separation. [0003] Due to the comp...

AI Technical Summary

Problems solved by technology

The protein structure of animal tissue is generally relatively loose. At the same time, in order to facilitate hydrolysis, the existing technology generally uses the raw material tissue to go through the process of cooking (above 120°C) to denature the protein structure, and then use trypsin, pepsin, papain, alkaline Conventional enzymes such as protease or medium-acid protease perform a single hydrolysis; at the same time, the separation methods used in the prior art are relatively simple, and the solid-liquid separation is carried out by using a cylindrical filter or a small plate-and-frame filter

[0003] Due to the compact structure of wheat protein, if any one of the above-mentioned single hydrolytic enzymes is used for hydrolysis according to the prior art, the degree of protein hydrolysis cannot reach the technical effect of the present invention; The high-temperature aging technology first will inevitably make the wheat protein into a semi-solidified colloidal state. On the one hand, the wheat protein in this semi-solidified state hinders the full contact between the hydrolyzed substrate and the enzymes, so that the hydrolysis reaction cannot be carried out well. On the one hand, due to the existence of semi-solidified colloids, it is difficult to filter the hydrolysis mother liquor, and the separation process is difficult to carry out; at the same time, the use of aging methods will inevitably destroy some useful (such as vitamins) heat-sensitive substances in the raw materials, thus affecting the product. The effect of using

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