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Method for recycling sucrose phospholylase in alpha-arbutin production process

A technology of sucrose phosphorylase and recovery method, which is applied in the field of sucrose phosphorylase recovery in the production process of α-arbutin, can solve the problems of reducing the use cost of sucrose phosphorylase, achieve recycling and reduce membrane pollution , increase the effect of stability

Active Publication Date: 2014-08-20
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for recovering sucrose phosphorylase in the production process of α-arbutin, effectively solving the recovery and utilization of free sucrose phosphorylase, so that the use cost of sucrose phosphorylase is significantly reduced, and for free sucrose The application of phosphorylase in the field of enzyme catalysis is of great significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The sucrose with a concentration of 20% (w / v), the hydroquinone with a concentration of 1.6% (w / v), and the sucrose phosphorylase enzyme solution with an enzyme activity of 200U / mL were placed in the reactor, and the pH was adjusted to 6. The reaction temperature was 25 °C, stirred slightly, and reacted for 24 hours. Under this reaction condition, the conversion rate of hydroquinone can reach 90%.

[0020] After the reaction is over, add trehalose equivalent to 5% (w / v) of the reaction solution into the reaction solution and stir evenly. First, use a ceramic membrane with a membrane pore size of 0.15 μm to filter out insoluble flocculent substances and some macromolecular proteins. Then, ultrafiltration is carried out using a cellulose acetate membrane with a molecular weight cut-off of 5000 Da to recover free sucrose phosphorylase. The recovery rate of sucrose phosphorylase reaches 97%, and the residual reactants and products in the recovered enzyme liquid are less th...

Embodiment 2

[0022] The sucrose with a concentration of 20% (w / v), the hydroquinone with a concentration of 1.6% (w / v), and the sucrose phosphorylase enzyme solution with an enzyme activity of 200U / mL were placed in the reactor, and the pH was adjusted to 6. The reaction temperature was 25 °C, stirred slightly, and reacted for 24 hours. Under this reaction condition, the conversion rate of hydroquinone can reach 90%.

[0023] After the reaction is over, add trehalose equivalent to 5% (w / v) of the reaction solution into the reaction solution and stir evenly. First, use a ceramic membrane with a membrane pore size of 0.1 μm to filter out insoluble flocculent substances and some macromolecular proteins. Then, ultrafiltration is carried out using a cellulose acetate membrane with a molecular weight cutoff of 2000Da to recover free sucrose phosphorylase. The recovery rate of sucrose phosphorylase reaches 93%, and the residual reactants and products in the recovered enzyme solution are less tha...

Embodiment 3

[0025] The sucrose with a concentration of 20% (w / v), the hydroquinone with a concentration of 1.8% (w / v), and the sucrose phosphorylase enzyme solution with an enzyme activity of 200U / mL were placed in the reactor, and the pH was adjusted to 6. The reaction temperature was 30 °C, stirred slightly, and reacted for 24 hours. Under this reaction condition, the conversion rate of hydroquinone can reach 85%.

[0026] After the reaction is over, add dextran equivalent to 5% (w / v) of the reaction solution into the reaction solution and stir evenly. First, use a ceramic membrane with a membrane pore size of 0.15 μm to filter out insoluble flocculent substances and some macromolecular proteins. , and then use a mixed fiber membrane with a molecular weight cut-off of 5000Da for ultrafiltration to recover free sucrose phosphorylase. The recovery rate of sucrose phosphorylase reaches 90%, and the residual reactants and products in the recovered enzyme solution are less than 5%.

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Abstract

The invention provides a method for recovering sucrose phosphorylase in the production process of α-arbutin. The α-arbutin production process uses sucrose and hydroquinone as substrates and utilizes free sucrose phosphorylase crude enzyme liquid. Used as a catalyst to catalyze the synthesis of α-arbutin. After the reaction is completed, a certain concentration of additives is first added to the reaction solution to improve its stability, and then a ceramic membrane is used to filter to remove insoluble floc substances and some macromolecular proteins, and then an ultrafiltration membrane with a smaller pore size is used to remove free sucrose phosphate. The recovered sucrose phosphorylase is finally used to carry out the next batch of catalytic reactions. Repeat the operation to achieve the purpose of reusing sucrose phosphorylase. The present invention proposes the recycling of sucrose phosphorylase for the first time, and this method is of great significance for reducing membrane pollution, extending the service life of the membrane, reducing production costs, etc., thereby making free sucrose phosphorylase very good in the field of enzyme catalysis. Application prospects.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a method for recovering sucrose phosphorylase in the production process of α-arbutin. Background technique [0002] Arbutin is a glucoside derivative of hydroquinone, which was first found in the leaves of plants of the genus Arbutin. Arbutin has physiological activities such as antitussive, expectorant, anti-inflammatory, and antibacterial, so it is used as antitussive drug and urinary tract disinfectant. More importantly, arbutin can inhibit the activity of tyrosinase, thereby curbing the growth of melanin, so it can be added to cosmetics as a whitening ingredient. There are two isomers of arbutin: α-arbutin and β-arbutin. Among them, the structure of α-arbutin is more stable than β-arbutin, the whitening effect is more than 10 times that of β-arbutin, and it has no effect on the normal growth of epidermal cells, and it is stable, effective and safe. Therefore, α-Arbutin is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/10
Inventor 马江锋万月佳贺爱永姜岷张敏陈可泉韦萍欧阳平凯
Owner NANJING TECH UNIV
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