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Method for preparing high-purity resveratrol from polygonum cuspidatum

A resveratrol, high-purity technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of small preparation, lower resveratrol yield, difficult industrialization, etc., to reduce loss, enhancement of market competitiveness, and simplification of operations

Inactive Publication Date: 2013-02-13
ENSHI QINGJIANG BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] CN 102531851 A adopts macroporous adsorption resin enrichment and semi-preparative high-performance liquid chromatography to separate and purify to obtain resveratrol and polydatin, but the amount prepared by semi-preparative high-performance liquid chromatography is too small to be industrialized and polydatin is not transformed For resveratrol, reduces the yield of resveratrol

Method used

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  • Method for preparing high-purity resveratrol from polygonum cuspidatum
  • Method for preparing high-purity resveratrol from polygonum cuspidatum
  • Method for preparing high-purity resveratrol from polygonum cuspidatum

Examples

Experimental program
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Effect test

Embodiment 1

[0091] (1) Crude extraction of resveratrol

[0092] Properly crush Polygonum cuspidatum to 20-40 meshes, weigh 100 kg of Polygonum cuspidatum powder in an extraction tank, reflux and stir with 60% (v / v) methanol aqueous solution 600 L at 50°C for 2 h, filter with a bag filter, and collect the filtrate In the storage tank, the residue was extracted twice according to the above steps, filtered, the filtrate was collected, and the filtrates were combined three times. The filter residue is squeezed dry with a squeeze dryer to collect the liquid, and the residue is recovered with a residue steamer to recover the residual solvent. The extracts were combined to obtain 1700 L of extracts. Transfer the extract to a vacuum concentration tank, concentrate in a vacuum at 55-60°C to recover methanol, and concentrate to no methanol. The specific gravity of the concentrate is 1.12, and the volume is about 240 L. The concentrate is transferred to an enzymolysis tank for enzymolysis.

[0093...

Embodiment 2

[0106] (1) Crude extraction of resveratrol

[0107] Properly crush Polygonum cuspidatum to 20-40 meshes, weigh 100 kg of Polygonum cuspidatum powder in an extraction tank, reflux and stir with 700 L of 80% (v / v) ethanol aqueous solution at 50°C for 3 h, filter with a bag filter, and collect the filtrate In the storage tank, the residue was extracted twice according to the above steps, filtered, the filtrate was collected, and the filtrates were combined three times. The filter residue is squeezed dry with a squeeze dryer to collect the liquid, and the residue is recovered with a residue steamer to recover the residual solvent. The extracts were combined to obtain 2000 L of extracts. Transfer the extract to a vacuum concentration tank, concentrate in a vacuum at 55-60°C to recover methanol, and concentrate to no methanol. The specific gravity of the concentrate is 1.15, and the volume is about 260 L. The concentrate is transferred to an enzymolysis tank for enzymolysis.

[01...

Embodiment 3

[0121] (1) Crude extraction of resveratrol

[0122] Properly crush Polygonum cuspidatum to 20-40 meshes, weigh 100 kg of Polygonum cuspidatum powder in an extraction tank, reflux and stir with 800 L of 70% (v / v) acetone aqueous solution at 60°C for 4 h, filter with a bag filter, and collect the filtrate In the storage tank, the residue was extracted twice according to the above steps, filtered, the filtrate was collected, and the filtrates were combined three times. The filter residue is squeezed dry with a squeeze dryer to collect the liquid, and the residue is recovered with a residue steamer to recover the residual solvent. The extracts were combined to obtain 2300 L of extracts. Transfer the extract to a vacuum concentration tank, concentrate in a vacuum at 55-60°C to recover methanol, and concentrate to no methanol. The specific gravity of the concentrate is 1.25, and the volume is about 270 L. The concentrate is transferred to an enzymolysis tank for enzymolysis.

[01...

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Abstract

The invention relates to a method for preparing high-purity resveratrol from polygonum cuspidatum. The method is characterized by comprising the following steps of (a) coarse extraction of the resveratrol: extracting the polygonum cuspidatum serving as a raw material through organic solvent; (b) enzymolysis: adding water into concentrated solution to disperse the concentrated solution, and then adding complex enzyme to perform the enzymolysis; (c) extraction: performing the extraction on the enzymolysis solution through solvent; (d) silica gel column chromatography purification of the resveratrol: performing equal gradient elution through eluent; (e) medium pressure C18 bonded silica gel octadecylsilyls (ODS) column chromatography purification: performing equal gradient elution through eluent mixed by methyl alcohol and water; and (f) performing recrystallization purification on a low-purity resveratrol sample IV through solvent. The purity of the resveratrol is higher than 98%, so that the resveratrol is up to the medicinal standard. The method for preparing the high-purity resveratrol from the polygonum cuspidatum is high in extraction rate, product purity and yield, short in production period, low in production cost and easy to operate and implement.

Description

technical field [0001] The invention relates to a method for preparing high-purity resveratrol from Polygonum cuspidatum. Background technique [0002] Polygonum cuspidatum, the dried rhizome and root of Polygonum cuspidatum Sieb. et Zucc. Polygonum cuspidatum is not only for viewing, but also for food. The tender stems are used as vegetables, and the roots are used as cold drinks. Its sap can dye rice noodles and has a unique flavor. It is also called "sour soup pole" because of its sour taste. Polygonum cuspidatum contains polygonum glycosides, organic acids, glucosides, polysaccharides, etc. It has the functions of clearing heat and detoxifying, cooling and detoxifying, invigorating the stomach and clearing food. [0003] Polygonum cuspidatum Sieb. et Zucc. is a traditional Chinese medicine in my country. It has the functions of lowering blood pressure, slowing heart rate, strengthening myocardial contractility, protecting the heart, and lowering blood fat; inhibiting...

Claims

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Application Information

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IPC IPC(8): C12P7/22C07C39/21C07C37/70
Inventor 向极钎程新华杨永康覃大吉殷红清张亮
Owner ENSHI QINGJIANG BIO ENG
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