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Novel caspase-14 activating peptides and compositions comprising said activating peptides

A composition, peptide compound technology, applied in the direction of drug combinations, peptide/protein components, peptides, etc.

Active Publication Date: 2016-01-20
ISP INVESTMENTS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no cosmetic compounds available that allow the use of caspase-14 to convert profilaggrin into filaggrin to be activated to protect the skin from UV radiation and improve barrier function, although there do exist The need for new care

Method used

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  • Novel caspase-14 activating peptides and compositions comprising said activating peptides
  • Novel caspase-14 activating peptides and compositions comprising said activating peptides
  • Novel caspase-14 activating peptides and compositions comprising said activating peptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Study of Caspase-14 Expression in Normal Human Keratinocytes in the Presence of Peptide SEQIDNo:2

[0067] The purpose of this study was to determine the expression of caspase-14 in normal human keratinocytes treated or not treated with the peptides of the invention:

[0068] Program

[0069] Cultured normal human keratinocytes NHK were treated with 1% or 3%-peptide SEQ ID No: 2 for 24 hours. Cells were then washed, fixed with 3.7%-paraformaldehyde, and diluted in BSA (diluted to 1 / 100 th) in the presence of 0.1%-triton was used. Cells were incubated in the presence of a caspase-14-specific mouse monoclonal antibody (BD Biosciences) followed by a secondary antibody conjugated to a fluorescent label. Cells were then examined by epifluorescence microscopy (Nikon Eclipse E600 microscope).

[0070] result

[0071] An increase in light intensity was observed in peptide-treated NHK cells relative to control conditions. This increase in fluorescence was do...

Embodiment 2

[0072] Example 2: Study of Caspase-14 Expression by siRNA in HNK Treated with Peptide SEQIDNo:2

[0073] To quantify the efficacy of the peptides of the invention on overexpression of caspase-14 in the NHK population, the gene encoding caspase-14 was "eliminated" using siRNA technology.

[0074] Program

Embodiment 4

[0075] NHK cells were cultured to 60% confluency in a 6-well plate, and then treated with 20 μL of 1%-peptide SEQ ID No:2. Then, carefully drop 100 μL of the previously prepared mixture of caspase-14-containing siRNA and transfection reagent well by well. Incubate the cell culture plate at 37°C and 5% CO 2 Incubate for 72 hours. Medium was changed every 2 days. Implement 4 conditions:

[0076] -Condition 1: Control without siRNA and peptide active ingredients

[0077] -Condition 2: siRNA transfected cells without peptide active ingredient

[0078] -Condition 3: Cells not transfected but treated with peptide active ingredient

[0079] -Condition 4: Cells transfected with siRNA and treated with peptide active ingredient

[0080] Quantification of caspase-14 expression was observed using conventional immunoblotting techniques (Western blotting) with anti-caspase-14 antibodies and according to conventional protocols. To analyze the compensation brought about by the peptides...

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PUM

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Abstract

The invention relates to peptidic compounds of general formula (I) R1-(AA)n-X1-X2-Ile-Gln-Ala-Cys-Arg-Gly-X3-(AA)p-R2 as caspase-14 activators. The invention also relates to a cosmetic or pharmaceutical composition comprising at least one peptide of general formula (I), in a physiologically acceptable medium, and to the use of said composition for preventing and / or repairing damage to deoxyribonucleic acid, for preventing and / or treating cutaneous signs of ageing and photo-ageing, and for improving the skin barrier function. The invention further relates to a cosmetic treatment method for preventing and / or treating cutaneous signs of ageing and photo-ageing, and for preventing and / or repairing damage caused by ultraviolet radiation.

Description

technical field [0001] The invention relates to the fields of cosmetics and medicines. The present invention relates to peptide compounds of the general formula (I) as caspase-14 activators: R 1 –(AA) n –X 1 –X 2 –Ile–Gln–Ala–Cys–Arg–Gly–X 3 –(AA) p –R 2 , and to their use in cosmetics and / or pharmaceuticals for preventing and / or correcting DNA damage, preventing and / or treating signs of skin aging and photoaging, and improving skin barrier function. Background technique [0002] The main function of the skin, the largest organ in the human body, is to protect the body from many forms of aggression, including external aggressions. Examples of such aggressions include such aggressions as pollution, UV radiation, or harsh chemicals such as surfactants, preservatives or fragrances, mechanical aggressions such as abrasions, shaving or depilation. Pollution means "external" pollution, for example due to diesel particles, ozone or heavy metals, and "internal" pollution, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K7/08A61K38/04A61K8/64A61Q17/00A61Q19/08
CPCA61K8/64A61K38/00A61P17/00A61Q17/04A61Q19/004A61Q19/08
Inventor C·达尔法拉N·东洛热J-M·博托
Owner ISP INVESTMENTS INC
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