Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of trimeresurus albolabris poison-resistant egg yolk antibody

A technology of egg yolk antibody and bamboo leaf green, applied in the field of biomedicine, to achieve the effect of high purity and preventing attack

Inactive Publication Date: 2013-03-13
CHONGQING NORMAL UNIVERSITY
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no specific medicine for the treatment of the venomous snakebite of the venomous snake, so the present invention provides a preparation method of the yolk antibody against the venom of the venom of the venomous snake

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of trimeresurus albolabris poison-resistant egg yolk antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] Preparation of Antibodies against Egg Yolk Antibodies from the Venom of A.

[0022] (1) Preparation of the venom antigen of the venomous snake: the adult venom was obtained by biting a plate, and then stored at low temperature after freeze-drying, and the LD of the venom was determined 50 .

[0023] (2) Emulsified adjuvant and immunized animals: After fully emulsifying the venom and complete Freund's adjuvant at a ratio of 1:1 (v / v), take 22-week-old egg-laying Lai aircraft carrier chickens (1.5±0.1kg), Injected subcutaneously at multiple sites in chicken wings, chest, abdomen and back. The initial immunization dose was 0.33 mg / monkey, and after 14 days and 22 days after immunization, it was changed to incomplete Freund's adjuvant for 2 booster immunizations, and the immune doses were 0.66 mg / bird and 1.32 mg / bird respectively; Eggs were numbered and stored at 4°C, and the eggs before immunization were used as negative controls. Unitary cages are adopted, one hen is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of a trimeresurus albolabris poison-resistant egg yolk antibody, which comprises the following steps of: (1) preparing a trimeresurus albolabris poison antigen from the poison of the adult trimeresurus albolabris, and injecting the antigen into a laying hen; and after immunization, collecting the egg and storing at low temperature; and (2) extracting and purifying by a water dilution method, an ammonium sulfate salt fractionation method and thiophilic chromatography to obtain the trimeresurus albolabris poison-resistant egg yolk antibody. Through the method disclosed by the invention, the prepared trimeresurus albolabris poison-resistant egg yolk antibody has high purity and can effectively suppress attack of the trimeresurus albolabris poison against organisms.

Description

technical field [0001] The invention belongs to the field of biomedicine. Specifically, the invention provides a preparation method of an anti-Villium venom yolk antibody for the treatment drug of the venomous snakebite. Background technique [0002] Venomous snake bites are sudden, severe, progress rapidly and have a high mortality rate. Applying antivenom as soon as possible is the most effective way to treat snake bites. Snake venom serum is used to immunize horses with detoxified snake venom. After the plasma is digested with gastric enzymes and salted out with ammonium sulfate, it is further refined and purified to make an antivenom preparation whose main component is IgG with Fc fragment removed. Antivenin horse serum has a large molecular weight, high price, inconvenient circulation, and complicated separation and preparation, especially the horse-derived IgG heterologous protein in the preparation, so allergic reactions often occur, and the common symptoms are rash, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K16/02
Inventor 和七一余晓东段海龙
Owner CHONGQING NORMAL UNIVERSITY