Tobacco virus disease quick rubbing inoculation method

A tobacco virus and rapid technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow speed, time-consuming and laborious, unsuitable for inoculation and identification of large groups of samples, etc., and achieves the effect of easy material collection and simple operation.

Inactive Publication Date: 2013-03-20
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the identification of tobacco virus disease resistance, conventional juice friction inoculation is mostly used. This method has the advantages of uniform and accurate inoculation, but it needs to be rubbed inoculated plant by plant, and the speed is too slow; Only good spray gun technology can achieve uniform inoculation; the inoculation of virus vectors is only suitable for viruses transmitted by virus vectors such as aphids, and it is necessary to raise non-toxic virus vectors, and then pass the virus acquisition, virus persistence and virus transmission. Waiting for multiple stages, time-consuming and laborious
In addition, the above traditional methods are not suitable for vaccination identification of large population samples.

Method used

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  • Tobacco virus disease quick rubbing inoculation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 Clothes brush friction inoculation compares with conventional friction inoculation incidence

[0023] 1.1 Test material

[0024] The inoculated varieties are Sansheng-NN and NC89. First, sow seedlings in plastic trays of 30cm×20cm. When the tobacco seedlings grow to 2-3 true leaves, they are artificially planted in 30-hole seedling trays and placed neatly in the seedling trays. Qi Nei. Wait until the tobacco seedlings grow to 5-6 true leaves, and spray 600-mesh quartz sand evenly before the test.

[0025] 1.2 Test method

[0026] Clothes brush friction inoculation: After washing hands with soapy water, use a pre-sterilized plastic clothes brush to soak up the virus juice and gently rub the leaves of the tobacco seedlings, so as to cause slight damage to the epidermal cells without death. Every time the virus juice is dipped, rub it back and forth 3 times, and each brush is 60cm long.

[0027] Routine rubbing inoculation: After washing hands with soapy w...

Embodiment 2

[0033] Embodiment 2 Clothes brush friction inoculation compares with routine friction inoculation time

[0034] 2.1 Test material

[0035] 1600 mutant germplasms for TMV, CMV and PVY resistance identification.

[0036] 2.2 Test method

[0037] The method of raising seedlings is the same as in Example 1, 30 plants are planted per share, 800 copies of which are inoculated with conventional plant-by-plant rubbing for the three viruses, and the other 800 are inoculated with rapid friction, and the inoculation time is counted.

[0038] 2.3 Test results

[0039] The test results show that with the same 800 materials and 24,000 tobacco seedlings, it takes 1017 minutes for 4 people to inoculate by conventional plant-by-plant friction, while it takes 103 minutes for one person to inoculate quickly with a plastic brush, saving 40 times the labor. Moreover, the rapid frictional inoculation with a plastic clothes brush is simple and rapid in operation, and the inoculation success rate ...

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Abstract

The invention discloses a tobacco virus disease quick rubbing inoculation method which includes a breeding step, a preparing virus liquid step and an inoculating step. Quartz sands are evenly sprayed on true leaves of tobacco seedlings, and then a disinfection clothes brush is dipped with the virus liquid to slightly rub the tobacco leaves, and the proper result is that epidermis cells of the tobacco leaves are slightly damaged and do not die. The cloth brush is used to dip the virus liquid one time, and then rub the tobacco leaves for three times back and forth, and each time can brush for 60 cm of the tobacco leaves. materials for experiments are easy to take, and operation is simple, time-saving, labor-saving, accurate and practical. The success rate of inoculation is 100%.

Description

technical field [0001] The invention belongs to the technical field of tobacco virus inoculation, and in particular relates to a rapid friction inoculation method for tobacco virus disease. Background technique [0002] As a model plant, tobacco is widely used in the field of biotechnology research, and the completion of tobacco genome sequencing will be more conducive to the biotechnology research of tobacco as a model plant. With the launch of the major project of the Tobacco Genome Project, the research project "Creation, Screening and Analysis of Tobacco Mutant Library" created and harvested 150,000 second-generation tobacco mutant seeds through chemical and biological methods, and comprehensively carried out the tobacco mutant library anti- Rapid, high-throughput vaccination screening and identification of TMV, CMV, and PVY. In the face of a large test group, the screening method should be fast, simple and accurate. [0003] At present, the inoculation method of tobac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01G7/06
Inventor 钱玉梅申莉莉王凤龙杨金广战怀旭
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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