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Luciferase gene-Lewis lung carcinoma (Luc-LLC) tumor model used for evaluating drug treating effects and establishment thereof and applications thereof

A treatment effect, LLC cell technology, applied in the Luc-LLC tumor model and its establishment and application field, can solve the problems of large influencing factors, complicated operation, inconvenient research, etc., and achieve reliable results, intuitive and convenient observation, and good application prospects Effect

Inactive Publication Date: 2013-03-20
SUZHOU RES INST OF TONGJI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the current transplanted lung cancer models need to be evaluated by anatomical methods to evaluate the efficacy of drugs. The operation is complicated, and the factors affected by human operation are large, which is not convenient for preclinical research.

Method used

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  • Luciferase gene-Lewis lung carcinoma (Luc-LLC) tumor model used for evaluating drug treating effects and establishment thereof and applications thereof
  • Luciferase gene-Lewis lung carcinoma (Luc-LLC) tumor model used for evaluating drug treating effects and establishment thereof and applications thereof
  • Luciferase gene-Lewis lung carcinoma (Luc-LLC) tumor model used for evaluating drug treating effects and establishment thereof and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0040] Step 1. Cloning of luciferase gene and construction of lentiviral vector:

[0041]After designing specific primers for the firefly luciferase (Firefly-Luciferase) gene, the Luciferase cDNA was amplified by PCR using the plasmid carrying the Luciferase gene as a template (Beiyuntian Company). The amplified Luciferase gene was successfully inserted into the PCR-Blunt vector, transformed into competent Escherichia coli cells, and the plasmid was extracted. After double digestion with XbaI and SalI, the Luciferase gene fragment was purified and recovered, and the fragment was combined with the lentiviral eukaryotic expression vector PRRL- The CMV empty plasmid (Backbone) was connected, the plasmid was extracted after the competent E. coli cells were transformed, the double enzyme digestion identification and sequencing results all proved that the Luciferase-PRRL-CMV expression vector was successfully constructed, and the sequence was confirmed to be completely correct (about...

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Abstract

The invention relates to a luciferase gene-Lewis lung carcinoma (Luc-LLC) tumor model used for evaluating drug treating effects and establishment of the Luc-LLC tumor model and applications of the Luc-LLC tumor model. According to the Luc-LLC tumor model, a firefly luciferase gene is introduced into human LLC cell lines by adoption of a gene recombination technology and a lentivirus infection, tumor cloned cell lines capable of translating the luciferase is acquired through subcloning selection, and then recombinant LLC cells are adopted to be inoculated in small mice, and the Luc-LLC tumor model is constructed. Compared with evaluating methods of a traditional tumor model used for evaluating the drug treating effects, the t Luc-LLC umor model is more intuitive and convenient to observe, vive-observation can be carried out, animals are not damaged, result is more reliable, and the Luc-LLC tumor model is good in application prospects .

Description

technical field [0001] The invention belongs to the field of tumor models and the establishment and application thereof, and in particular relates to a Luc-LLC tumor model which can be used to evaluate the effect of drug treatment and the establishment and application thereof. Background technique [0002] Lung cancer is the number one killer threatening the health of our people. According to the "2010 China Health Statistical Yearbook" issued by the Ministry of Health, in 2009, malignant tumors ranked first among all causes of death in my country. Statistics show that among the top ten malignant tumors with the highest mortality rate, lung cancer ranks first. Development carries a serious economic burden. In recent years, the treatment and basic research of lung cancer have made great progress, but the current basic and preclinical research of lung cancer drugs still lacks a reliable in vivo and in vitro research platform that can more conveniently evaluate the efficacy of ...

Claims

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Application Information

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IPC IPC(8): A01K67/02C12N15/867
Inventor 房健民周爽杨洋李栋
Owner SUZHOU RES INST OF TONGJI UNIV
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