Compound astragalus polysaccharide liposome long-acting injection and preparation method thereof

A technology of astragalus polysaccharide and injection, applied in the directions of liposome delivery, antiviral agent, drug combination, etc., can solve the problems of easy moisture absorption, poor stability, decreased egg production of laying hens, etc., and achieve good sustained release. effect, improved stability, good histocompatibility

Inactive Publication Date: 2013-03-20
HENAN SOAR VETERINARY PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, astragalus polysaccharide powder is easy to absorb moisture, has poor stability, and is difficult to preserve. Astragalus polysaccharide injection is an injection drug commonly used in the adjuvant treatment of chicken infectious bursal disease in the 03 edition of "Quality Standards for Veterinary Drugs". Intramuscular injection, once a day, for two days in a row. According to the interview, the injection for two days in a row will cause severe stress response in chickens, causing a significant drop in egg production of laying hens

Method used

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  • Compound astragalus polysaccharide liposome long-acting injection and preparation method thereof
  • Compound astragalus polysaccharide liposome long-acting injection and preparation method thereof
  • Compound astragalus polysaccharide liposome long-acting injection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: 1g of astragalus polysaccharide was dissolved in pH 7.4 phosphate buffer, and then added to a solution made by dissolving 5g lecithin for injection, 1g cholesterol, 0.1g dicetyl phosphate, 0.01g spironolactone in chloroform , Evaporate to remove chloroform, disperse the residual liquid by ultrasonic to separate the liposomes, resuspend in 100ml pH7.4 phosphate buffer to obtain a light yellow liposome suspension, and then filter with 0.45μm microporous membrane 2 Second, 0.22μm aseptically filtered and then subpackaged to obtain compound astragalus polysaccharide long-acting injection.

Embodiment 2

[0016] Example 2: 1.5g of astragalus polysaccharide was dissolved in pH 7.4 phosphate buffer, and then added to the ether made by dissolving 6g distearoylphosphatidylglycerol, 2g cholesterol, 0.5g dicetyl phosphate, 0.015g spironolactone In the resulting solution, the ether was removed by evaporation, the residual liquid was dispersed by ultrasonic, the liposomes were separated, and resuspended in 100ml pH7.4 phosphate buffer to obtain a light yellow liposome suspension, then 0.45μm micropores Filter it twice with a filter membrane, 0.22μm aseptically filtered and then subpackaged to obtain compound astragalus polysaccharide long-acting injection.

Embodiment 3

[0017] Example 3: 2g of astragalus polysaccharide was dissolved in pH 7.4 phosphate buffer, and then added to a solution made by dissolving 7g of lecithin for injection, 2g of cholesterol, 0.5g of dicetyl phosphate, and 0.02g of spironolactone in chloroform , Evaporate to remove chloroform, disperse the residual liquid by ultrasonic to separate the liposomes, resuspend in 100ml pH7.4 phosphate buffer to obtain a light yellow liposome suspension, and then filter with 0.45μm microporous membrane 2 Second, 0.22μm aseptically filtered and then subpackaged to obtain compound astragalus polysaccharide long-acting injection.

[0018] In the above embodiments, the PBS buffer is prepared according to conventional methods in the art, for example, 1L, pH 7.4 PBS formula: potassium dihydrogen phosphate (KH 2 PO 4 ): 0.27g, disodium hydrogen phosphate (Na 2 HPO 4 ): 1.42g, sodium chloride (NaCl): 8g, potassium chloride (KCl) 0.2g, add about 800mL of deionized water to fully stir to dissolve, t...

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Abstract

The invention discloses a compound astragalus polysaccharide liposome long-acting injection and a preparation method thereof, belonging to the technical field of medicinal preparations. Each 100 ml of the injection comprises the following effective components: 1 to 2 g of astragalus polysaccharide, 5 to 7 g of phosphatide, 1 to 3 g of cholesterol, 0.1 to 1 g of dihexadecyl phosphate and 0.01 to 0.02 g of spironolactone. According to the compound astragalus polysaccharide liposome long-acting injection provided by the invention, inherent properties of phosphatide and cholesterol are utilized and certain external means are used to wrap astragalus polysaccharide by using liposome so as to obtain a bimolecular structure similar to a biomembrane, and the injection has good histocompatibility and cell affinity, improves stability of astragalus polysaccharide and presents a good slow releasing effect; in clinical application, compared with original astragalus polysaccharide injection under the condition of same usage amount, one application of the injection provided by the invention can realize a good effect which is obtained when conventional injection is applied twice.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to a compound astragalus polysaccharide liposome long-acting injection and a preparation method thereof. Background technique [0002] Liposomes are mainly composed of phospholipids and cholesterol. Phospholipids are ubiquitous in the protoplasm and biofilms of animal and plant cells. They are one of the main components of cell membranes and play an important role in regulating the physiological activity of biofilms and the normal metabolism of the body. [0003] Cholesterol is a neutral lipid that is abundant in various animal cells. It is mainly combined with phospholipids in the membrane to change the properties of the phospholipid layer. It tends to weaken the connection between lipids and proteins in the membrane, and its main role is to regulate the fluidity of the membrane. [0004] As a carrier, liposomes are valuable in that they can contai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/715A61P31/12A61P37/04
Inventor 贺培益陈圆圆刘升柴保国高义
Owner HENAN SOAR VETERINARY PHARMA
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