Method for producing functional microorganism organic fertilizer by using food residue
A technology of functional microorganisms and production methods, applied in organic fertilizers, climate change adaptation, etc., to reduce energy consumption and investment, promote seed germination, and improve germination potential and emergence rate.
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Embodiment 1
[0028] Collect fresh kitchen waste, separate solid and liquid to obtain solid waste and liquid waste, and pick out impurities such as broken glass and plastic in solid waste.
[0029] 1. Preparation of Candida tropicalis bacterial solution: Candida tropicalis (purchased from China Center for Type Culture Collection (CCTCC), its preservation number: CCTCC AY92045) was cultured from Candida tropicalis slant Pick a loop from the base and inoculate it into the liquid Candida seed medium, at 28°C, 150rpm, shake and culture for 24h, and check the purity by microscopy. Then the inoculum of 3% (v / v) was inserted into the fermenter filled with Candida seed medium, the temperature was 28°C, the stirring speed was 250rpm, and the ventilation rate was 3L / (L·min) , the fermentation time is 24 hours to obtain Candida tropicalis bacteria liquid, the Candida slant medium contains glucose 5g / L, peptone 2g / L, yeast extract 1g / L, sodium chloride 0.5g / L, agar 20g / L, the balance is water, pH is 7...
Embodiment 2
[0044] Collect fresh kitchen waste, separate solid and liquid to obtain solid waste and liquid waste, and pick out impurities such as broken glass and plastic in the solid waste.
[0045] 1. Preparation of Candida tropicalis bacterial liquid: Pick a ring of Candida tropicalis from the Candida slant medium and inoculate it into the liquid Candida seed medium, and inoculate at 30°C , 150rpm, shake culture for 48h, microscopically check the purity, the strain is in the logarithmic growth phase, stop the culture, and obtain the first-class strain of Candida tropicalis; In the fermenter of Trichosanthes seed culture medium, the temperature is 30°C, the stirring speed is 200rpm, the ventilation rate is 2L / (L·min), and the fermentation time is 24 hours, to obtain the Candida tropicalis bacteria liquid, the Candida tropicalis Bacteria slant medium contains glucose 5g / L, peptone 2g / L, yeast extract 1g / L, sodium chloride 0.5g / L, agar 20g / L, the balance is water, pH is 6.5, sterilization...
Embodiment 3
[0056] Collect fresh kitchen waste, separate solid and liquid to obtain solid waste and liquid waste, and pick out impurities such as broken glass and plastic in the solid waste.
[0057] 1. Preparation of Candida tropicalis bacterial liquid: Pick a ring of Candida tropicalis from the Candida slant medium and inoculate it into the liquid Candida seed medium, and incubate at 37°C , 200rpm, shake culture for 12h, microscopically check the purity, the strain is in the logarithmic growth phase, stop the culture, and obtain the first-class strain of Candida tropicalis; In the fermenter of the trichotillomyces seed medium, the temperature is 35°C, the stirring speed is 300rpm, the ventilation rate is 4L / (L·min), and the fermentation time is 72 hours, to obtain the Candida tropicalis liquid, the Candida tropicalis The slant medium contains glucose 5g / L, peptone 2g / L, yeast extract 1g / L, sodium chloride 0.5g / L, agar 20g / L, the balance is water, pH is 7.5, sterilization conditions: ste...
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