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Sandwich type electrochemical immunosensor, preparation method and application thereof

An immunosensor, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve the effects of high catalytic performance, high sensitivity, and fast detection speed

Active Publication Date: 2013-11-13
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the dumbbell type Pt-Fe 3 o 4 The construction of electrochemical immunosensors using nanoparticles to detect multiple tumor markers has not been reported yet

Method used

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  • Sandwich type electrochemical immunosensor, preparation method and application thereof
  • Sandwich type electrochemical immunosensor, preparation method and application thereof
  • Sandwich type electrochemical immunosensor, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Preparation of Sandwich Electrochemical Immunosensor

[0054] (1) Synthesis of nitrogen-doped graphene

[0055] Synthesis of graphene oxide: 0.3 g of graphite and 1.8 g of potassium permanganate were mixed, put into a three-necked flask with a magnet, and 40 mL of a mixture of concentrated sulfuric acid and phosphoric acid with a volume ratio of 9:1 was added to the In the above three-neck bottle. Put the three-neck flask into an oil bath, heat it to 50 °C, and react for 12 hours. After the reaction is over, pour the sample onto about 40 mL of ice, and at the same time, put the magnet on the poured ice-water mixture, and slowly magnetically stir , add 0.3 mL of 30% hydrogen peroxide, the ice melts gradually, and magnetically stir for 0.5 h. After the reaction, the obtained mixture was centrifuged at 8000 R for 0.5 h, then washed three times with 20 mL of water, 20 mL of 30% hydrochloric acid, and 20 mL of ethanol, discarded the supernatant, and finally was...

Embodiment 2

[0073] Example 2 Detection of cervical cancer tumor markers: SCC, CA12-5 or CEA

[0074] The electrochemical immunosensor was prepared according to the steps described in Example 1. The electrochemical immunosensor was used to detect the cervical cancer tumor markers shown in Table 1, and the detection technical indicators are shown in Table 1.

[0075] Table 1 Detection technical indicators of cervical cancer tumor markers

[0076]

[0077] It can be seen from the detection technical index results in Table 1 that the electrochemical immunosensor prepared by the present invention is used for the detection of cervical cancer tumor markers, and has a wide linear range, low detection limit and high method sensitivity.

Embodiment 3

[0078] Example 3 Detection of gastric cancer tumor markers: CA72-4, CEA or CA19-9

[0079] The electrochemical immunosensor was prepared according to the steps described in Example 1, and the gastric cancer tumor markers shown in Table 2 were detected by using the electrochemical immunosensor, and the detection technical indicators are shown in Table 2.

[0080] Table 2 Detection technical indicators of gastric cancer tumor markers

[0081]

[0082] It can be seen from the results of detection technical indicators in Table 2 that the electrochemical immunosensor is used for the detection of gastric cancer tumor markers, which has a wide linear range, low detection limit, and high sensitivity of the method.

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Abstract

The invention relates to an electrochemical immunosensor, a preparation method and application thereof, in particular to a sandwich type electrochemical immunosensor constructed by a marker based on dumbbell type Pt-Fe3O4 nano particles serving as a second antibody, a preparation method thereof, and application of the electrochemical immunosensor prepared by using the method in measurement of tumor markers. The electrochemical immunosensor is high in accuracy, stability, reproducibility and sensitivity, can quickly and conveniently perform immunoassay detection and can be used for clinical analysis.

Description

technical field [0001] The present invention relates to a kind of electrochemical immunosensor, its preparation method and application thereof, more specifically, the present invention relates to a kind of sensor based on dumbbell type Pt-Fe 3 o 4 A sandwich-type electrochemical immunosensor constructed with nanoparticles as a marker of a secondary antibody, a preparation method thereof, and an application of the electrochemical immunosensor prepared by the method in determining tumor markers. Background technique [0002] The presence or content changes of tumor markers can indicate the nature of the tumor and help to understand the tissue origin and cell differentiation of the tumor, thereby helping the pathological diagnosis, histological classification, prognosis and guiding clinical treatment of tumors. Its discovery and application has become one of the commonly used methods for clinical detection of tumors after imaging diagnosis and pathological diagnosis, and it is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327G01N33/574
Inventor 吴丹张勇魏琴范海霞杜斌
Owner UNIV OF JINAN
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