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Method for recovering herbicide atrazine-polluted soil by using degradable bacteria

A technology of atrazine and contaminated soil, applied in the field of contaminated soil remediation, to achieve the effect of reducing operation steps

Inactive Publication Date: 2013-03-27
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In 1996, foreign countries authorized a patent for atrazine-contaminated soil bioremediation. The bacterial strain used was Pseudomonas sp.ADP (U.S. patent5508193, Pseudomonas strain for degradation of s-triazines in soil and water.issuedApril16, 1996) ; At present, there is no domestic patent for bioremediation of atrazine-contaminated soil with bacteria

Method used

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  • Method for recovering herbicide atrazine-polluted soil by using degradable bacteria
  • Method for recovering herbicide atrazine-polluted soil by using degradable bacteria
  • Method for recovering herbicide atrazine-polluted soil by using degradable bacteria

Examples

Experimental program
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Embodiment 1

[0021] Example 1 Remediation of Atrazine Contaminated Soil by Arthrobacter AD32 and Pseudomonas AD35 Mixed Bacteria (Low Concentration Cells)

[0022] 1. Collect the cells from 20mL Arthrobacter AD32 and Pseudomonas AD35 by centrifugation respectively, wash the cells with 0.9% NaCl solution and sterile water successively, suspend the cells in sterile water after centrifugation, and then divide the cells at a ratio of 1:1 Ratio (OD 600 Numerically equal) mixed, after centrifugation, the cells were suspended in a medium containing 3g / L sucrose and 0.3g / L phosphate (0.2g Na 2 HPO 4 12H 2 O, 0.1g KH 2 PO 4 ) solution, so that the cell concentration is 10 4 individual / mL.

[0023] 2. Add 10 g of sterilized soil, 2 mg of atrazine, and 500 μL of AD32 and AD35 mixed bacterial suspension into a sterile petri dish with a diameter of 9 cm, and incubate at 30°C for 10 days. Pseudomonas ADP cells were used as a positive control, and soil not inoculated with bacteria was used as a ne...

Embodiment 2

[0026] Example 2 Remediation of Atrazine-Contaminated Soil by Mixed Bacteria of Arthrobacter AD32 and Pseudomonas AD35 (High Concentration Cells)

[0027] 1. Collect the cells from 20mL Arthrobacter AD32 and Pseudomonas AD35 by centrifugation respectively, wash the cells with 0.9% NaCl solution and sterile water successively, suspend the cells in sterile water after centrifugation, and then divide the cells at a ratio of 1:1 Ratio (OD 600 Numerically equal) mixed, after centrifugation, the cells were suspended in a medium containing 3g / L sucrose and 0.3g / L phosphate (0.2g Na 2 HPO 4 12H 2 O, 0.1g KH 2 PO 4 ) solution, so that the cell concentration is 10 8 individual / mL.

[0028] 2. Add 10 g of sterilized soil, 2 mg of atrazine, and 500 μL of AD32 and AD35 mixed bacterial suspension into a sterile petri dish with a diameter of 9 cm, and incubate at 30°C for 5 days. Pseudomonas ADP cells were used as a positive control, and soil not inoculated with bacteria was used as a...

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Abstract

The invention relates to a method for separating and identifying two bacterial strains of degradable herbicide atrazine, and carrying out bioremediation on polluted soil with an atrazine concentration of 200mg / kg by using mixed bacteria of the two bacterial strains, belonging to the field of polluted soil recovery. The used bacterial strains are respectively arthrobacterium (Arthrobacter sp.) AD32 and pseudomonas (Pseudomonas sp.) AD35; a proper amount of cane sugar (1g / L-3g / L) and phosphate (0.3g / L) are added into the bacterium suspension when the soil is recovered; and after the soil is processed for two days in 30 DEG C, the removing rate of the atrazine is 100%.

Description

technical field [0001] The invention belongs to the field of contaminated soil remediation, and relates to the separation and identification of two bacterial strains that degrade the herbicide atrazine, and a method for bioremediating atrazine-contaminated soil with the two strains of bacteria. Background technique [0002] Atrazine is a triazine herbicide with the trade name atrazine, which is mainly used for weed control in corn, sorghum and sugarcane fields. Atrazine has been produced and used in my country since the early 1980s, and the current annual output is about 20,000 tons. The mass production and use of this herbicide has caused pollution to soil, groundwater and surface water. Studies have shown that atrazine is an endocrine disruptor, which can interfere with the regulatory function of hormones, cause reproductive defects in humans and amphibians, and induce tumors and cancers. Atrazine residues in soil at high levels can affect the growth of crop rotations and...

Claims

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Application Information

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IPC IPC(8): B09C1/10
Inventor 牛淑敏郭巧云蔡宝立
Owner NANKAI UNIV
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