Method for recovering herbicide atrazine-polluted soil by using degradable bacteria
A technology of atrazine and contaminated soil, applied in the field of contaminated soil remediation, to achieve the effect of reducing operation steps
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Embodiment 1
[0021] Example 1 Remediation of Atrazine Contaminated Soil by Arthrobacter AD32 and Pseudomonas AD35 Mixed Bacteria (Low Concentration Cells)
[0022] 1. Collect the cells from 20mL Arthrobacter AD32 and Pseudomonas AD35 by centrifugation respectively, wash the cells with 0.9% NaCl solution and sterile water successively, suspend the cells in sterile water after centrifugation, and then divide the cells at a ratio of 1:1 Ratio (OD 600 Numerically equal) mixed, after centrifugation, the cells were suspended in a medium containing 3g / L sucrose and 0.3g / L phosphate (0.2g Na 2 HPO 4 12H 2 O, 0.1g KH 2 PO 4 ) solution, so that the cell concentration is 10 4 individual / mL.
[0023] 2. Add 10 g of sterilized soil, 2 mg of atrazine, and 500 μL of AD32 and AD35 mixed bacterial suspension into a sterile petri dish with a diameter of 9 cm, and incubate at 30°C for 10 days. Pseudomonas ADP cells were used as a positive control, and soil not inoculated with bacteria was used as a ne...
Embodiment 2
[0026] Example 2 Remediation of Atrazine-Contaminated Soil by Mixed Bacteria of Arthrobacter AD32 and Pseudomonas AD35 (High Concentration Cells)
[0027] 1. Collect the cells from 20mL Arthrobacter AD32 and Pseudomonas AD35 by centrifugation respectively, wash the cells with 0.9% NaCl solution and sterile water successively, suspend the cells in sterile water after centrifugation, and then divide the cells at a ratio of 1:1 Ratio (OD 600 Numerically equal) mixed, after centrifugation, the cells were suspended in a medium containing 3g / L sucrose and 0.3g / L phosphate (0.2g Na 2 HPO 4 12H 2 O, 0.1g KH 2 PO 4 ) solution, so that the cell concentration is 10 8 individual / mL.
[0028] 2. Add 10 g of sterilized soil, 2 mg of atrazine, and 500 μL of AD32 and AD35 mixed bacterial suspension into a sterile petri dish with a diameter of 9 cm, and incubate at 30°C for 5 days. Pseudomonas ADP cells were used as a positive control, and soil not inoculated with bacteria was used as a...
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