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Method for comprehensively extracting polysaccharides, polyphenol and saponin from camellia oleifera abel defatted cakes

A technology of camellia oleifera and camellia oleifera, which is applied in the field of comprehensive extraction of polysaccharides, polyphenols and saponins, can solve the problems of low purity of saponins and inability to separate and obtain active substances, and achieve a wide range of raw material sources, low production costs, and simple operations. Effect

Active Publication Date: 2013-03-27
GUANGXI NORMAL UNIV
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] In the traditional direct water extraction method of saponin, polysaccharides, phenols and other substances affect the purification process of saponin, resulting in low purity of saponin, and at the same time, it is impossible to separate and obtain active substances such as polysaccharides and phenols

Method used

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  • Method for comprehensively extracting polysaccharides, polyphenol and saponin from camellia oleifera abel defatted cakes

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Add 50 g of camellia oleifera powder to 450 ml of petroleum ether (boiling range 60-90°C), heat at 47°C for 2.5 h to remove fat and residual oil, recover petroleum ether, and dry to obtain defatted camellia oleifera powder. Water was added to the obtained degreased camellia oleifera powder, so that the mass volume ratio of camellia oleifera powder and water was 1:20 g / ml, leached continuously at 70 °C for 3.5 h, cooled, and centrifuged. Concentrate the water extract to 1 / 4 of the original volume, add 5% hydrochloric acid to adjust the pH of the solution to 4.0, centrifuge at a speed of 4500 rpm for 10 min, keep the supernatant, and then add 95% ethanol to it to make the ethanol The final concentration of the mixture was 75%, stirred, allowed to stand, and the precipitate obtained by centrifugation was freeze-dried to obtain camellia oleifera polysaccharide. The supernatant after the precipitation of polysaccharides was concentrated to 1 / 4 of the original volume, and eth...

Embodiment 2

[0028] Add 800 ml of petroleum ether (boiling range 60-90°C) to 100 g of camellia oleifera powder, heat at 50°C for 2 h to remove fat and residual oil, recover petroleum ether, and dry to obtain defatted camellia oleifera powder. Water was added to the obtained degreased Camellia oleifera powder, so that the mass volume ratio of Camellia oleifera powder and water was 1:15, the extraction was continued at 75 °C for 3 h, cooled, and centrifuged. Concentrate the water extract to 1 / 3 of the original volume, add 8% hydrochloric acid to adjust the pH of the solution to be 3.5, centrifuge at a speed of 3500 rpm for 15 min, keep the supernatant, and then add 95% ethanol to it to make the ethanol The final concentration was 80%, stirred, left standing, and the precipitate obtained by centrifugation was freeze-dried to obtain camellia oleifera polysaccharide. The supernatant after the polysaccharide precipitation was concentrated to 1 / 5 of the original volume, KCl solid was added to the...

Embodiment 3

[0031]Add 3000 ml of petroleum ether (boiling range 60-90°C) to 500 g of camellia oleifera powder, heat at 60°C for 2 h to remove fat and residual oil, recover petroleum ether, and dry to obtain defatted camellia oleifera powder. Water was added to the obtained degreased Camellia oleifera powder, so that the mass volume ratio of Camellia oleifera powder and water was 1:10, the extraction was continued at 80 °C for 2 h, cooled, and centrifuged. The water extract is concentrated to 1 / 2 of the original volume, adding 10% sulfuric acid to adjust the pH of the solution to be 3.0, centrifuging at a speed of 3000rpm for 15min, retaining the supernatant, and then adding 95% ethanol to it to make the final concentration of ethanol was 75%, stirred, left standstill, and the precipitate obtained by centrifugation was freeze-dried to obtain camellia oleifera polysaccharide. The supernatant after the polysaccharide precipitation was concentrated to 1 / 5 of the original volume, and NaCl soli...

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Abstract

The invention provides a method for comprehensively extracting polysaccharides, polyphenol and saponin from camellia oleifera abel defatted cakes. The method includes the following steps: obtaining polysaccharides through aqueous extraction, concentration, deproteinization and ethanol precipitation after the camellia oleifera abel defatted cakes are degreased; concentrating and adding salt into the supernatant after the ethanol precipitation of the polysaccharides, and performing extraction to obtain the polyphenol through ethyl acetate; and absorbing and analyzing the aqueous phase after extraction of the polyphenol through resin to obtain the saponin. According to the method, effects of substances such as the polysaccharides and the polyphenol during extraction of the saponin are overcome, and the purity of the saponin is improved; and the polysaccharides, the polyphenol and the saponin are obtained, and the defect of low utilization rate of camellia oleifera abel defatted cakes in the existing methods is overcome.

Description

technical field [0001] The invention relates to a method for comprehensively extracting polysaccharides, polyphenols and saponins from camellia oleifera, and belongs to the field of extraction and deep processing of active ingredients of camellia oleifera. Background technique [0002] Camellia ( Camellia oleifera Abel. ) belongs to the genus Camellia in the family Theaceae, and is mainly distributed in southern regions such as Guangxi, Hunan, and Jiangxi in my country. It is one of the four traditional woody oil plants that are as famous as olives, oil palms, and coconuts. Camellia oleifera is the residue left after oil extraction of camellia oleifera fruit, also known as tea seed cake, tea bran and other names. Camellia oleifera is a by-product of tea oil production, its yield is nearly three times that of camellia oil, and it is rich in biologically active substances such as saponin, polysaccharides, and polyphenols, and has good development and utilization in the fields...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H15/256C07H1/08C07J63/00C07G99/00
Inventor 覃江克高石花李怡杰万广朋明娜陈珊
Owner GUANGXI NORMAL UNIV
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