Method for performing screening in virus-sensitive cell cloning by applying indirect immunofluorescence assay technology

A technology for immunofluorescence detection and virus-sensitive cells, applied in the biological field, can solve the problems of high cost, long cycle, complicated operation, etc., and achieve the effect of short detection time, strong repeatability and consistent effect.
CN102994445AActive Publication Date: 2013-03-27山东滨州沃华生物工程有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
山东滨州沃华生物工程有限公司
Publication Date
2013-03-27

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Abstract

The invention relates to the field of biotechnology, and particularly relates to a method for performing screening in virus-sensitive cell clonal strains by applying an indirect immunofluorescence assay technology. The method comprises the following steps of: identifying the purity of a cell line; obtaining monoclonal cell strains in the cell line; and identifying the sensitivity of the monoclonal cell strains to virus. The method disclosed by the invention has the beneficial effects of being short in detection time, capable of identifying the sensitivity of single-cell cloning to virus only by 3 days, relative simple to operate, easy, capable of being used for screening lots of non-cytopathic virus-sensitive cell strains, stable in result, strong in repeatability, and consistent with the effect of a virulence determination method.
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Description

technical field

[0001] The invention relates to the field of biotechnology, and more specifically relates to a method for screening virus-sensitive cell clones using indirect immunofluorescence detection technology. Background technique

[0002] Traditionally, the screening of sensitive cell lines is achieved by measuring the virus titer. However, for non-pathogenic viruses, the determination of the toxicity can only be carried out by using animals or other methods, such as the determination of the attenuated toxicity of classical swine fever rabbit. This method is time-consuming, labor-intensive, complicated to operate, long in cycle, high in cost and low in screening efficiency. Contents of the invention

[0003] In view of the above deficiencies, the present invention provides a simple and quick screening method for sensitive cell lines, specifically, the identification of the purity of cell lines and the sensitivity identification of different monoclonal cell lines by...

Claims

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