Method for producing xylitol through fermenting lees hydrolysate by using pichia guilliermondii

A yeast fermentation and hydrolyzate technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of seriousness, pollution, and high production process costs, and achieve fast reaction, strong anti-interference ability, and product separation. handy effect

Inactive Publication Date: 2013-03-27
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] The present invention overcomes the problems of high cost and serious pollution in the existing xylitol production process existing in the background technology, and provides a method for producing xylitol by fermenting distiller's grain hydrolyzate by Pichia mongolica

Method used

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  • Method for producing xylitol through fermenting lees hydrolysate by using pichia guilliermondii
  • Method for producing xylitol through fermenting lees hydrolysate by using pichia guilliermondii

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Embodiment 1

[0029] (1) Preparation of distiller's grain hydrolyzate

[0030] Grind the dried distiller's grains to a particle size of 40 mesh, mix it with 0.1% NaOH solution at a ratio of 1:5 (w / v), heat and boil for 1 h under stirring, filter with a filter cloth after cooling, discard the filtrate, and use Wash the filter residue with clean water, then boil the filter residue with 1000 mL of water for 1 h, filter, and remove the filter residue after removing ash to be hydrolyzed in the next step; use a mixture of 1% HCl and 1% H 3 PO 4 The composed mixed acid hydrolyzed distiller's grains, the hydrolysis temperature is 100°C, the solid-to-liquid ratio is 1:10 (w / v), hydrolyzed for 2 h; the hydrolyzate is concentrated at 85°C until the xylose content is about Ratio (w / v) adding activated carbon for decolorization, concentration and decolorization can increase the initial xylose concentration in the hydrolyzate, and also remove harmful substances such as organic acids, furfural and pigmen...

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Abstract

The invention relates to a method for producing xylitol through fermenting lees hydrolysate by using pichia guilliermondii, which mainly solves the problem that the existing xylitol production is high in cost and serious in pollution. The method comprises the following steps: (1) lees hydrolysate preparation; (2) medium preparation; and (3) xylitol fermentation: shake flask fermentation: liquid seeds are inoculated to a fermentation medium, wherein the optimal process of shake flask culture is as follows: the loaded liquid content is 1 / 5, the inoculum concentration is 5%, the temperature is 28 DEG C, the rotating speed is 180 r / min, and the fermentation is performed for 48h so as to obtain xylitol containing fermentation liquor; and the fermentation liquor is determined by using high performance liquid chromatograph, the concentration of xylitol in the xylitol containing fermentation liquor is 9.016 g / L, and the xylitol content of the xylitol containing fermentation liquor subjected to chromatographic separation can reach 95%. The method for producing xylitol through fermenting lees hydrolysate by using pichia guilliermondii is mild in process conditions, low in energy consumption, low in environmental pollution, and safer and more reliable in product quality, and improves the application efficiency of vinasse which is a salvaged material of agricultural and sideline products.

Description

technical field [0001] The invention relates to a process for producing xylitol by fermentation, in particular to a process for producing xylitol by fermenting distiller's grain hydrolyzate by Pichia mongolica. Background technique [0002] Xylitol (Xylitol), also known as pentapentitol, is a five-carbon sugar alcohol. Its molecular formula is C 5 h 12 o 5 , is a normal intermediate product of xylose metabolism, the shape is crystalline white powder, it can be used as sweetener, nutrient and pharmaceutical widely used in chemical, food, pharmaceutical and other industries. At the same time, xylitol is a non-cariogenic sweetener, which has been widely used in food, medicine and other fields; its metabolism in the body does not require the participation of insulin, so it is suitable as a sweet substitute for diabetic patients. The raw materials for the production of xylitol mostly come from agricultural and sideline products such as corn cobs, cottonseed hulls, bagasse, bi...

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Application Information

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IPC IPC(8): C12P7/18C12R1/84
Inventor 张丽媛姚笛王颖李秀波胡亚光
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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