Preparation based on tumor marker sensor of sodamide group smectite and application

A sodium amide-based montmorillonite and tumor marker technology, applied in the field of functional materials and biosensing, can solve the problems of complex instruments, cumbersome detection process and high cost, and achieve high sensitivity, good specificity and simple method. Effect

Inactive Publication Date: 2013-03-27
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] One of the technical tasks of the present invention is to overcome the deficiencies in the existing technology for detecting tumor markers, such as the shortcomings of complex instruments, high cost, and cumbersome detection process, and provide A method for preparing a highly sensitive and specific electrochemical immunosensor, the preparation technology of which is mature and reliable;

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  • Preparation based on tumor marker sensor of sodamide group smectite and application
  • Preparation based on tumor marker sensor of sodamide group smectite and application
  • Preparation based on tumor marker sensor of sodamide group smectite and application

Examples

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Embodiment 1

[0067] The preparation of the tumor marker sensor based on sodium amide montmorillonite comprises the following steps:

[0068] (1) At 30°C, a silver-gold alloy film with a gold / silver mass ratio of 1:1 was floated in 10 mol L -1 After 4 min on the surface of the nitric acid solution, the silver was etched away to form a nanoporous gold film. The nanoporous gold membrane was washed with ultrapure water until its pH = 7.0.

[0069] (2) Weigh 10.00 g of montmorillonite, put it into a large beaker, add 100 mL of distilled water, stir for 2 h, place it on a stable laboratory table without vibration, and let it stand; after the layers are separated, take out the supernatant, It is montmorillonite suspension; add the above suspension to 250 mL of 0.50 mol L -1 In the NaCl solution, stir for 12 h and let stand; after the layers are separated, suck out the supernatant; add 250 mL of -1 NaCl solution, stirred for 12 h, let it stand still, sucked out the supernatant; put the montmori...

Embodiment 2

[0075] The preparation of the tumor marker sensor based on sodium amide montmorillonite comprises the following steps:

[0076] (1) At 30°C, a gold / silver mass ratio of 1:1.1 silver-gold alloy film was floated in 12 mol L -1 On the surface of nitric acid solution for 5 min, the silver was etched away to form a nanoporous gold film. The nanoporous gold membrane was washed with ultrapure water until its pH = 7.0.

[0077](2) Weigh 10.00 g of montmorillonite, put it into a large beaker, add 100 mL of distilled water, stir for 2.5 h, place it on a stable laboratory table without vibration, and let it stand; after stratification, take out the supernatant, It is montmorillonite suspension; add the above suspension to 250 mL of 0.50 mol L -1 In NaCl solution, stir for 18 h and let stand; after the layers are separated, suck out the supernatant; add 250 mL of -1 NaCl solution, stirred for 12 h, let it stand still, sucked out the supernatant; put the montmorillonite suspension in a ...

Embodiment 3

[0083] The preparation of the tumor marker sensor based on sodium amide montmorillonite comprises the following steps:

[0084] (1) At 35 °C, a gold / silver weight ratio of 1:1.2 silver-gold alloy film was floated in 15 mol L -1 After 7 min on the surface of the nitric acid solution, the silver was etched away to form a nanoporous gold film. The nanoporous gold membrane was washed with ultrapure water until its pH = 7.0.

[0085] (2) Weigh 10.00 g of montmorillonite, put it into a large beaker, add 100 mL of distilled water, stir for 3 h, place it on a stable laboratory table without vibration, and let it stand still. After layering, take out the supernatant, which is the montmorillonite suspension. Add the above suspension to 250 mL of 0.50 mol L -1 NaCl solution, stirred for 24 h and allowed to stand. After the layers were separated, aspirate the supernatant; add 250 mL of 0.50 mol L -1 NaCl solution, stirred for 12 h, allowed to stand, and sucked out the supernatant. T...

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Abstract

The invention discloses a sandwich type electrochemistry immunosensor which is prepared based on a sodamide group smectite and a nanometer multi-hole gold membrane, and belongs to the technical field of function materials and biological sense. The sandwich type electrochemistry immunosensor provided by the invention can carry out amido functionalization on the sodium group smectite, an antibody and enzyme are conveniently fixed, and meanwhile, the favorable biological activities of the antibody and the enzyme are kept; a favorable three-dimensional serial perforated structure and biocompatibility of the nanometer multi-hole gold membrane are utilized, and the favorable catalytical property of the antibody is directly fixed, and the sensitivity of the sensor is notably improved; and the sodium group smectite which is cheap and is easily available is utilized, and the cost of the sensor is greatly reduced. The sandwich type electrochemistry immunosensor provided by the invention can realize high sensitiveness and specificity and rapid exact detection of multiple tumor markers.

Description

technical field [0001] The invention relates to the preparation and application of a tumor marker sensor based on sodium amide-based montmorillonite. Specifically, it is a sandwich-type electrochemical immunosensor based on sodium amide-based montmorillonite and nanoporous gold film, which is used to measure various tumor markers, and belongs to the technical field of functional materials and biosensing. Background technique [0002] Tumor markers have important practical value in the early diagnosis of cancer. Tumor markers are a class of substances produced by tumor cells themselves or by the body's response to tumor cells during the occurrence and proliferation of tumor cells, reflecting the existence and growth of tumors. Including proteins, hormones, enzymes, polyamines and oncogene products. Tumor markers are widely used in clinical practice, and play an extremely important role in cancer screening in normal population, auxiliary diagnosis of cancer in symptomatic pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N27/26
Inventor 魏琴杜斌辛晓东吴丹张勇李贺王志玲马洪敏
Owner UNIV OF JINAN
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