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Quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I, and preparation method of kit

A chemiluminescence immunoassay and angiotensin technology, which is applied in the field of immunoassay medicine, can solve the problems of short validity period of reagents, narrow detection range, cumbersome operation, etc., and achieve the effect of good stability, easy operation and high sensitivity

Active Publication Date: 2013-03-27
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to provide Ang I magnetic particle chemiluminescence immunoquantitative detection kit and its preparation method, which avoids the shortcomings of radioimmunoassay reagents such as short validity period, radioactive pollution, and cumbersome operation, and solves the problem of low sensitivity, Detect narrow, costly defects

Method used

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  • Quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I, and preparation method of kit
  • Quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I, and preparation method of kit
  • Quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I, and preparation method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of angiotensin Ⅰ (Ang Ⅰ) magnetic particle chemiluminescence immunoquantitative determination kit Ⅰ

[0032] (1) Preparation of Ang Ⅰ calibrator:

[0033] Dilute the pure Ang Ⅰ with 50% bovine serum into a series of gradients with concentrations of 0, 0.8, 2, 10, 20, 50 ng / mL;

[0034] (2) Preparation of Ang Ⅰ quality control product:

[0035] The Ang Ⅰ antigen was used to prepare low-value quality control products and high-value quality control products containing 50% bovine serum, the concentrations of which were 4.16ng / mL and 17.4ng / mL respectively;

[0036] (3) Preparation of magnetic particle-streptavidin suspension:

[0037] Take 100mL of 0.1mol / L 2-morpholine ethanesulfonic acid solution (MES solution), add 10mg magnetic particles and 3mg streptavidin (SA) in sequence, stir for 30min, then add 10mg / mL carbodiimide hydrochloride Solution (1-ethyl-(3-dimethylaminopropyl, EDC) 3.5uL, after 1h reaction, use a magnetic stand to absorb, stand for 10min, re...

Embodiment 2

[0059] Example 2: Preparation of angiotensin Ⅰ (Ang Ⅰ) magnetic particle chemiluminescence immunoquantitative determination kit Ⅱ

[0060] (1) Preparation of Ang Ⅰ calibrator:

[0061] Dilute the pure Ang Ⅰ with 50% bovine serum into a series of gradients with concentrations of 0, 0.8, 2, 10, 20, 50 ng / mL;

[0062] (2) Preparation of Ang Ⅰ quality control products:

[0063] The Ang Ⅰ antigen was used to prepare low-value quality control products and high-value quality control products with 50% bovine serum, the concentrations of which were 6.24ng / mL and 11.6ng / mL;

[0064] (3) Preparation of magnetic particle-streptavidin suspension:

[0065] Take 100mL of 0.1mol / L 2-morpholine ethanesulfonic acid solution (MES solution), add 10mg magnetic particles and 3mg streptavidin (SA) in sequence, stir for 30min, then add 10mg / mL carbodiimide hydrochloride Solution (1-ethyl-(3-dimethylaminopropyl, EDC) 3.5uL, after 1h reaction, use a magnetic stand to absorb, stand for 10min, remove the liquid, a...

Embodiment 3

[0076] Example 3: Preparation of angiotensin Ⅰ (Ang Ⅰ) magnetic particle chemiluminescence immunoassay kit Ⅲ

[0077] (1) Preparation of Ang Ⅰ calibrator:

[0078] Dilute the pure Ang Ⅰ with 50% bovine serum into a series of gradients with concentrations of 0, 0.8, 2, 10, 20, 50 ng / mL;

[0079] (2) Preparation of Ang Ⅰ quality control products:

[0080] The Ang Ⅰ antigen was used to prepare low-value quality control products and high-value quality control products containing 50% bovine serum, the concentrations of which were 5.2ng / mL and 13.8ng / mL respectively;

[0081] (3) Preparation of magnetic particle-streptavidin suspension:

[0082] Take 100mL of 0.1mol / L 2-morpholine ethanesulfonic acid solution (MES solution), add 10mg magnetic particles and 3mg streptavidin (SA) in sequence, stir for 30min, then add 10mg / mL carbodiimide hydrochloride Solution (1-ethyl-(3-dimethylaminopropyl, EDC) 3.5uL, after 1h reaction, use a magnetic stand to absorb, stand for 10min, remove the liquid, add ...

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Abstract

The invention discloses a quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for angiotensin (Ang) I. The kit comprises Ang I calibrators, magnetic particle suspension coupled with streptavidin, an Ang I antibody labeled with biotin, an Ang I enzyme combination, an Ang I quality controller, chemiluminescence liquor A, chemiluminescence liquor B, 20 times concentrated washing liquor, and a reaction tube, wherein enzyme adopted by the Ang I enzyme combination is horse radish peroxidase with the purity RZ being more than or equal to 3.0 and the activity being more than or equal to 250U / ml. The invention also discloses a preparation method of the kit. Compared with the conventional kit, the quantitative detection kit is simple and convenient to operate, is safe, does not cause environment pollution, and also has the advantages of wide concentration range, low cost, good stability and the like of detection samples.

Description

Technical field [0001] The invention relates to the field of immunoassay medicine. Specifically, the invention provides an angiotensin I (AngI) magnetic particle chemiluminescence immunoquantitative detection kit and a preparation method thereof. Background technique [0002] Angiotensin I is transformed from the action of renin on angiotensinogen, so it can reflect renin activity. The renin-angiotensin-aldosterone system plays an important role in maintaining blood pressure, water and electrolyte balance, and internal environment stability. [0003] Angiotensin I can stimulate the adrenal medulla to secrete epinephrine, and it has no obvious effect on constricting blood vessels directly; it can promote the secretion of aldosterone from the adrenal cortex, which acts on the renal tubules to retain sodium, water, and potassium, thereby causing increased blood volume , Blood pressure rises, clinically, angiotensin I is of great significance in the diagnosis and classification of hyp...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/531G01N33/532G01N21/76
Inventor 刘萍张影宋启超范利花
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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