Method for detection of infection with human cytomegalovirus

Abstract

Disclosed is a novel and highly practically available means for detecting the infection with an HCMV with high sensitivity. A full-length protein of each of 15 kinds of HCMV proteins is synthesized, and the reactivity of the full-length protein with serum collected from each of HCMV infection patients is extensively examined. As a result, it is found that all of infection patients can be detected completely when a full-length pp28 protein is used as an antigen. The full-length pp28 protein can be synthesized in a large amount in the form of a recombinant protein and purified using Escherichia coli, and can be used for commercial purposes as an antigen for use in the test on HCMVs.

Description

technical field [0001] The invention relates to a detection method for human cytomegalovirus infection. Background technique [0002] In serological diagnosis of infectious diseases caused by human cytomegalovirus (HCMV), viral antigens (natural antigens) are generally used. However, in natural antigens, batch-to-batch differences due to preparation methods cannot be ignored. In addition, since the virus itself is dealt with, there are also problems in terms of safety and simplicity. [0003] In order to standardize the serological diagnosis of HCMV, it is hoped to realize the recombination of the antigen, but it is known that the sensitivity and specificity of the test are not sufficient when only the recombinant antigen is used. This is because it is difficult to select an appropriate antigen suitable for recombination from a plurality of viral antigens. In addition, the use of partially expressed fragments of the respective antigenic proteins is considered to be anothe...

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Problems solved by technology

However, it has been reported that when only partially expressed fragments are used, the sensitivity of the test is about 60%, which is not sufficient.

[0005] However, it is also known that in the field of serum examination using immunoassay, although the use of full-length antigen is necessary, the detection sensitivity does not necessarily improve

For example, in Non-Patent Document 1, the antibody reactivity of the serogroup of HCMV-positive patients was studied for the natural antigen of HCMV (including the full-length viral protein), and the result of evaluating the antibody reactivity for each protein was given, but Only pp150 can achieve a detection sensitivity close to 100%. Depending on the type of protein, the detection sensitivity becomes very low, reaching less than 50%.

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