Method for extracting phenylethanoid glycoside and flavonoid C-glycoside from corallodiscus flabellata

A technology of flavonoid carbon glycosides and phenethyl alcohol glycosides is applied in the directions of pharmaceutical formulations, non-central analgesics, medical preparations containing active ingredients, etc., to achieve the effects of simple method, easy operation and high efficiency

Inactive Publication Date: 2013-04-03
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a method for extracting phenylethanol glycosides and flav...

Method used

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  • Method for extracting phenylethanoid glycoside and flavonoid C-glycoside from corallodiscus flabellata
  • Method for extracting phenylethanoid glycoside and flavonoid C-glycoside from corallodiscus flabellata
  • Method for extracting phenylethanoid glycoside and flavonoid C-glycoside from corallodiscus flabellata

Examples

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Effect test

Embodiment 1

[0010] Take Lithophora medicinal material, add ethanol with a mass concentration of 50%, the weight-to-volume ratio of medicinal material and ethanol is 1:12, reflux extraction at 80°C for 2.5 hours each time, filter, and recover the filtrate under reduced pressure Solvent, vacuum-dried to obtain extract, standby, get HPD-100 macroporous adsorption resin and carry out pretreatment, pretreatment method is, the mass concentration of HPD-100 macroporous adsorption resin is that the mass concentration of 0.5BV is 95% ethanol soaking 24h (BV is 1 resin bed volume), with 2BV of ethanol with a mass concentration of 95% at 2BV·h -1 The flow rate is passed through the resin column, and the resin is soaked for 4h, and the mass concentration is 95% ethanol 2BV·h -1 Wash the resin at a flow rate of 5% hydrochloric acid solution with a mass concentration of 2BV at 5BV·h -1 The flow rate passes through the resin layer, and the resin is soaked for 3 hours, and then washed with water to neut...

Embodiment 2

[0012] Take the medicinal material of Lithium gallbladder, add ethanol with a mass concentration of 10%, the weight volume ratio of the medicinal material of Lithium gallbladder and ethanol is 1:8, reflux extraction once at 50°C, each extraction time is 1.5h, filter, and the filtrate is decompressed Reclaim solvent, vacuum-dry to get extract, standby, get HPD-100 macroporous adsorption resin and carry out pretreatment, pretreatment method is, the mass concentration of HPD-100 macroporous adsorption resin is that the mass concentration of 0.5BV is 95% ethanol soaking 24h (BV is 1 resin bed volume), with 2BV of ethanol with a mass concentration of 95% at 2BV·h -1 The flow rate is passed through the resin column, and the resin is soaked for 4h, and the mass concentration is 95% ethanol 2BV·h -1 Wash the resin at a flow rate of 2BV until the effluent has no white turbidity when adding water, and then wash with water at the same flow rate until there is no alcohol smell; use 5% hyd...

Embodiment 3

[0014] Take the herba olidocarpi, add ethanol with a mass concentration of 95%, the weight-to-volume ratio of the herb and ethanol is 1:16, reflux extraction at 90°C for 3.5 hours each time, filter, and recover the filtrate under reduced pressure Solvent, vacuum-dried to obtain extract, standby, get HPD-100 macroporous adsorption resin and carry out pretreatment, pretreatment method is, the mass concentration of HPD-100 macroporous adsorption resin is that the mass concentration of 0.5BV is 95% ethanol soaking 24h (BV is 1 resin bed volume), with 2BV of ethanol with a mass concentration of 95% at 2BV·h -1 The flow rate is passed through the resin column, and the resin is soaked for 5h, and the mass concentration is 95% ethanol 2BV·h -1 Wash the resin at a flow rate of 2BV until the effluent has no white turbidity when adding water, then wash with water at the same flow rate until there is no alcohol smell; use 5% hydrochloric acid solution with a mass concentration of 2BV to ...

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Abstract

The invention relates to a method for extracting phenylethanoid glycoside and flavonoid C-glycoside from corallodiscus flabellate, effectively solving the problem of extraction of the phenylethanoid glycoside and the flavonoid C-glycoside from the corallodiscus flabellate. The method comprises the following steps: taking a Chinese herb corallodiscus flabellate; adding ethanol, and performing reflux extraction; filtering; decompressing filtrate, and recycling a solvent; drying the filtrate in vacuum to obtain an extract for use; taking macroporous adsorption resin, and pretreating the macroporous adsorption resin; dynamically adsorbing the extract by the pretreated resin column; after complete adsorption, rinsing the resin column with distilled water, and eluting for impurity removal; after the impurity removal, eluting with ethanol till eluent is colorless; and collecting the eluent, thus obtaining the phenylethanoid glycoside and the flavonoid C-glycoside. The method is simple, easy to operate, low in extraction cost and high in extraction efficiency.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, in particular to a method for extracting phenylethanol glycosides and flavonoid carbon glycosides in Lithophora chinensis. Background technique [0002] As one of the traditional Chinese medicines, shidancao is recorded in "Yunnan Chinese Herbal Medicine" and "Kunming Folk Commonly Used Herbal Medicine": "Bitter, pungent, cold. Promote blood circulation and detoxification, reduce swelling and relieve pain. Treat irregular menstruation, red and white vaginal discharge, Heart palpitations, bruises, knife wounds, sores, carbuncles, and stubborn ringworm." "Clearing heat and detoxification, dehumidification. Treating damp-heat arthralgia, mumps, and sore throat." In modern times, Lithophorus is commonly used in the early stage of the disease in the Funiu Mountain area of ​​western Henan. Respiratory tract infection and treatment of irregular menstruation, leucorrhea and other symptoms in ...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P31/04A61P31/12A61P1/16A61P29/00A61P39/06A61P37/02A61P25/28A61P9/10
Inventor 王彦志冯卫生郑晓珂刘媛媛王小兰
Owner HENAN UNIV OF CHINESE MEDICINE
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